Downregulation of miR-544 in tissue, but not in serum, is a novel biomarker of malignant transformation in glioma

Wang

et al. 2016

Biological Chemistry

Triple negative breast cancer lacking estrogen receptor (ER), progesterone receptor and Her2 account for account for the majority of the breast cancer deaths, due to the lack of specific gene targeted therapy. Our current study aimed to investigate the role of miR-544 in triple negative breast cancer. Endogenous levels of miR-544 were significantly lower in breast cancer cell lines than in human breast non-tumorigenic and mammary epithelial cell lines. We found that miR-544 directly targeted the 3'-untranslated region (UTR) on both Bcl6 and Stat3 mRNAs, and overexpression of miR-544 in triple negative breast cancer cells significantly down-regulated expressions of Bcl6 and Stat3, which in turn severely inhibited cancer cell proliferation, migration and invasion in vitro. Employing a mouse xenograft model to examine the in vivo function of miR-544, we found that expression of miR-544 significantly repressed the growth of xenograft tumors. Our current study reported miR-544 as a tumor-suppressor microRNA particularly in triple negative breast cancer. Our data supported the role of miR-544 as a potential biomarker in developing gene targeted therapies in the clinical treatment of triple negative breast cancer.

Section: Discussionmentioning

confidence: 99%

MicroRNA-544 down-regulates both Bcl6 and Stat3 to inhibit tumor growth of human triple negative breast cancer

Wang

et al. 2016

Biological Chemistry

J of Cellular Biochemistry

“…The expression pattern of the miR‐449 cluster resembles that of miR‐34b/c during spermatogenesis, and the miR‐449 cluster may have the same roles in the regulation of male germ cell development as miR‐34b/c [Bao et al, ]. miR‐544, as one of the 18 miRNAs in the 14q32 miRNA‐clusters, regulates both cell proliferation and the cell cycle in various cancers and may be a diagnostic marker of both gastric cancer and glioblastoma [Ma et al, ; Zhi et al, ]. However, there is little information on miR‐544 regulation of TFs in SSCs.…”

Section: Discussionmentioning

confidence: 99%

“…miR-21 is important in maintaining the spermatogonia population and for spermatogonia self-renewal [Niu et al, 2011]. miR-34c regulates the differentiation of mouse embryonic stem cells into male germ-like cells through RARg and promotes SSC differentiation by targeting Nanos2 [Yu et al, 2014;Zhang et al, 2012]. The expression pattern of the miR-449 cluster resembles that of miR-34b/c during spermatogenesis, and the miR-449 cluster may have the same roles in the regulation of male germ cell development as miR-34b/c [Bao et al, 2012].…”

Section: Discussionmentioning

confidence: 99%

“…miR‐544, as one of the 18 miRNAs in the 14q32 miRNA‐clusters, regulates both cell proliferation and the cell cycle in various cancers and may be a diagnostic marker of both gastric cancer and glioblastoma [Ma et al, ; Zhi et al, ]. However, there is little information on miR‐544 regulation of TFs in SSCs and stem cells.…”

mentioning

confidence: 99%

“…However, there remains a lack of information as to whether some miRNAs regulated the PLZF in the self-renewal and differentiation of male germline stem cells. miR-544, as one of the 18 miRNAs in the 14q32 miRNAclusters, regulates both cell proliferation and the cell cycle in various cancers and may be a diagnostic marker of both gastric cancer and glioblastoma [Ma et al, 2012;Zhi et al, 2013]. However, there is little information on miR-544 regulation of TFs in SSCs and stem cells.…”

mentioning

confidence: 99%

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miR‐544 Regulates Dairy Goat Male Germline Stem Cell Self‐Renewal via Targeting PLZF

Song

et al. 2015

The balance between the self-renewal and differentiation of male germline stem cells (mGSCs) is critical for the initiation and maintenance of mammalian spermatogenesis. The promyelocytic leukemia zinc finger (PLZF), a zinc finger protein, is a critical factor for maintaining the self-renewal of mGSCs, so, evaluation of the PLZF pathway in mGSCs may provide a deeper insight into mammalian spermatogenesis. miRNA was also an important regulating factor for the self-renewal and differentiation of mGSCs; however, there is currently no data indicating that which miRNA regulate the self-renewal and differentiation of mGSCs via PLZF. Here, we predicted the prospective miRNA targeting to PLZF using the online Bioinformatics database-Targetscan, and performed an analysis of the dual-luciferase recombinant vector, psiCHCEKTM-2-PLZF-3'UTR. miR-544 mimics (miR-544m), miR-544 inhibitors (miR-544i), Control (NC, scrambled oligonucleotides transfection), pPLZF-IRES2-EGFP or PLZF siRNA were transfected into mGSCs; the cells proliferation was evaluated by BRDU incorporation assay and flow cytometry, and the mGSC marker, GFRa1, PLZF, KIT, DAZL, and VASA expression were analyzed by RT-qPCR, immunofluorescence and Western blot. The results showed that miR-544 regulates dairy goat male germline stem cell self-renewal via targeting PLZF. Our study identifies a new regulatory pathway for PLZF and expands upon the PLZF regulatory network in mGSCs.

Extensive miRNA expression analysis in craniopharyngiomas

Samis

Vanin²,

Bonaldo³

et al. 2016

Childs Nerv Syst