Downregulation of miR‑588 is associated with tumor progression and unfavorable prognosis in patients with osteosarcoma

Abstract

Osteosarcoma (OS) is a primary malignant tumor characterized by a high metastatic potential and poor prognosis. The dysregulation of miR-588 has been demonstrated to serve crucial roles in the progression of numerous types of cancer. The present study aimed to investigate the expression and function of miR-588 in the development of OS. To do so, clinical samples were collected and analyzed, and in vitro experiments were conducted. A total of 104 patients with OS were recruited between 2012 and 2014. The expression of miR-588 was analyzed by reverse transcription quantitative PCR. The association between miR-588 expression and the clinicopathological characteristics and survival rate of patients with OS was evaluated. Furthermore, Cell Counting Kit-8 and Transwell assays were used to evaluate the effect of miR-588 on the proliferation and the migratory and invasive abilities of various OS cell lines. The results demonstrated that miR-588 expression in OS tissues and cells was significantly lower compared with normal tissues and cells. In addition, miR-588 expression was closely associated with the Musculoskeletal Tumor Society (MSTS) staging of patients with OS. miR-588 expression and MSTS staging were therefore considered as independent indicators for the prognosis of patients with OS. In addition, miR-588 downregulation significantly stimulated the proliferation and migratory and invasive abilities of OS cells. Taken together, these findings indicated that miR-588 may serve as an independent prognostic factor and tumor suppressor in OS.

CircECE1 promotes osteosarcoma progression through regulating RAB3D by sponging miR-588

“…Furthermore, miR-421 is able to control MCPIP1 expression through negative regulation, thus promoting OS tumorigenicity in vivo [ 33 ]. Our study confirmed that miR-588 is down-regulated in OS cells, consistent with recent literature reports [ 19 , 34 ]. MiR-588 downregulation is reported to be tightly correlated with Musculoskeletal Tumor Society (MSTS) staging in OS patients [ 19 ].…”

“…Our study confirmed that miR-588 is down-regulated in OS cells, consistent with recent literature reports [ 19 , 34 ]. MiR-588 downregulation is reported to be tightly correlated with Musculoskeletal Tumor Society (MSTS) staging in OS patients [ 19 ]. MiR-588 downregulation also dramatically enhances the proliferation, migration and invasive abilities of OS cells [ 19 ].…”

“…demonstrated that miR-337-3p contributes to OS cell proliferation, migration and invasion via regulation of zinc finger protein 652 (ZNF652) expression [ 18 ]. Meanwhile, miR-588 can act as a tumor suppressor and modulate the poor prognosis of OS patients [ 19 ].…”

Analysis of microRNA expression in CD133 positive cancer stem‑like cells of human osteosarcoma cell line MG-63

“…The Wnt signaling pathway plays important roles in the progression and development of many cancers including OS (Liang et al, 2021;Taciak et al, 2018). The Wnt signaling pathway has been reported to be activated in OS (Chen et al, 2015).…”

“…Osteosarcoma (OS) is a primary malignant tumor of bone occurring in young adults, with a morbidity of 4,000,000 per year ( Li et al, 2016 ; Yin et al, 2021 ). A combination of surgery with adjuvant and neoadjuvant chemotherapy has been developed to improve the 5-year survival rate of OS patients; nevertheless, the prognosis of OS patients remains poor due to the high rates of tumor metastasis and recurrence, and drug resistance ( Liu et al, 2021 ; Ren et al, 2021 ; Yu et al, 2021 ; Zhang, Ma & Li, 2019a ). Therefore, the molecular mechanisms underlying metastasis, chemoresistance, and progression of OS need to be clarified to improve therapeutic options.…”