Doxironide Magnetic Nanoparticles for Selective Drug Delivery to Human Acute Myeloid Leukemia

El‐Boubbou, Kheireddine; Azar, Daniel Fadi; Bekdash, Amira; Abi-Habib, Ralph J.

doi:10.1166/jbn.2017.2365

Cited by 13 publications

(14 citation statements)

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“…This confirmed and explained the confocal microscopy results obtained. Our findings are consistent with the results of previous studies by us 72,73 and others 82,83 , reporting on the increased uptake of IONPs by human breast, leukemia and cervical cancer cells compared to normal cells. While this definitely requires further mechanistic studies, it is mostly attributed to the variations in cell-membrane compositions, characteristics, morphologies, immunoprofiles, and metabolic activities between the tested cancerous and normal cells 82–85 .…”

Section: Resultssupporting

confidence: 94%

“…5b inset). Such high loading efficiencies are much greater than drug incorporation onto nonporous polymer-coated iron oxide MNPs (~80–150 μg of Dox/mg of MNPs) reported in our previous work 72,73 . In fact, the high drug loading amounts of IO-MMM is chiefly attributed to its mesoporous nature with high surface area and pore volume packing the different drug molecules.…”

Section: Resultsmentioning

confidence: 67%

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Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

El‐Boubbou

Ali

Alzahrani

et al. 2019

Sci Rep

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The preparation of mesoporous iron oxides with controllable physiochemical properties for effective therapeutic drug delivery remains a formidable challenge. Herein, iron oxide mesoporous magnetic microparticles (IO-MMMs) were prepared by a modified reverse hard-templating approach using, for the first time, acid-prepared mesoporous spheres (APMS) as the hard silica template. The obtained mesostructures exhibited remarkably high surface area and large pore volumes ( S BET = 240 m 2 /g and V pore = 0.55 cm 3 /g), controllable average sizes, generally uniform morphologies, and excellent biocompatibilities, allowing them to achieve optimal drug release in cancer cells and tumor tissues. IO-MMM carriers were able to co-load high amounts of hydrophilic chemotherapeutic drugs (Dox or Daun) and/or hydrophobic hormonal anticancer drugs (Tam), and release them sustainably in a pH-dependent manner, utilizing the fluorescence of Daun to real-time trace the intracellular drug distribution, and employing Daun/Tam to treat cancer by combined chemo/hormonal therapy. Cytotoxicity assays against different types of cancerous cells showed that the combinatory Daun/Tam@IO-MMM formulation significantly reduced the viability of metastatic MCF7 and KAIMRC1 breast as well as HCT8 colorectal cancer cells, with the least potency towards non-cancerous normal primary cells (up to 10-fold). Electron, flow, and live confocal microscopy imaging confirmed that the loaded vehicles were successfully and differentially uptaken by the different tested cells, gradually releasing their payloads, and causing apoptotic cell death. Importantly, compared to free drugs, Daun/Tam@IO-MMMs displayed enhanced drug accumulation in patient breast primary tumor tissues, deeply penetrating into the tumor region and killing the tumor cells inside. The designed carriers described here, thus, constitute a novel promising magnetic mesoporous smart system that entraps different kinds of drugs and release them in a controlled manner for combinatorial chemo/hormonal cancer theranostics. This multifactorial platform may open new avenues in cancer therapy as efficient synergistic antitumor system through overcoming limitations of conventional cancer therapy.

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 67%

Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

El‐Boubbou

Ali

Alzahrani

et al. 2019

Sci Rep

Self Cite

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“…NPs are submicrometer moieties made of organic or inorganic materials and with unique characteristics compared to their bulk counterparts. Besides their intrinsic nanoscale physical phenomena, their often exhibit exceptional optical, electrical, magnetic, and chemical properties, attracting vast attention in many biotechnological areas, such as magnetic resonance imaging, drug delivery systems, hyperthermia and labeling and separation of cells and proteins, among other biological entities . Different kinds of NPs, such as quantum dots, carbon nanotubes, gold NPs, silica NPs, polymeric NPs, and magnetic NPs (MNPs) have been designed and synthesized.…”

Section: Magnetic Npsmentioning

confidence: 99%

Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

550

330

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Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area of biomedical engineering, recent and intense progress on magnetic nanoparticle research, from synthesis to surface functionalization strategies, demands for a work that includes, summarizes, and debates current directions and ongoing advancements in this research field. Thus, the present work addresses the structure, synthesis, properties, and the incorporation of magnetic NPs in nanocomposites, highlighting the most relevant effects of the synthesis on the magnetic and structural properties of the magnetic NPs and how these effects limit their utilization in the biomedical area. Furthermore, this review next focuses on the application of magnetic NPs on the biomedical field. Finally, a discussion of the main challenges and an outlook of the future developments in the use of magnetic NPs for advanced biomedical applications are critically provided.

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“…The as prepared MNPs have the capability to target the tumour tissues. El‐Boubbou et.al reported the preparation of chemotherapeutic agent formed of stabilized metal oxide NPs which was found to be more efficient than free DOX for the treatment of acute myeloid leukaemia (AML). Also,FG.…”

Section: Introductionmentioning

confidence: 99%

Fe₃O₄ @ PLGA‐PEG Nanocomposite for Improved Delivery of Methotrexate in Cancer Treatment

2018

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Magnetic Fe 3 O 4 nanoparticles are gaining significance in drug delivery applications owing to their targeting capability. Surface modification of amphiphilic block polymers by Fe 3 O 4 nanoparticles increases their properties. In this study, Fe 3 O 4 @ PLGA-PEG nanocomposite is prepared by double emulsion (w/ o/w) method. A shift in the 2Θ values for the composite in XRD attributes to interaction between Fe 3 O 4 and PLGA-PEG. Also, a shift in the Fe-O band in the FTIR spectrum of Fe 3 O 4 @PLGA-PEG from 578 cm -1 to 510 cm -1 confirms the formation of nanocomposite. Surface morphology of the prepared nanocomposite is analyzed by TEM and AFM. Decrease in agglomeration due to electrostatic repulsion between the polymer chains and magnetic particles is observed while an increased surface area (61.0nm) confirms the formation of the nanocomposite. To determine the effectiveness of the prepared magnetically modified nanoparticles, methotrexate (anticancer drug) is encapsulated into the nanocomposite. High entrapment effi-ciency of 95% is observed when polymer:drug is 1:1. The invitro release profile shows that pH of release medium plays a significant role. At physiological pH of 7.3 there is only 15% methotrexate release while nearly 86% of methotrexate release is observed at acidic pH of 4.6 over 72h. Korsemeyer-Peppas model of drug release (R 2 -0.9868) represents swelling controlled release of methotrexate. Further, the cytotoxic cell viability assay on SK-BR-3 (breast adinocarcinoma) cells showed that methotrexate loaded onto the nanocomposite showed higher cell viability as compared to free methotrexate after 96h of incubation. The fluorescent cell imaging also showed that methotrexate released slowly from the nanoparticles and diffused into the nucleus without losing its cytotoxic effect on the cancer cells. Based on these properties of the magnetically modified PLGA-PEG nanoparticles they can be used as targeting drug delivery agents in treatment of cancer therapy.

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Doxironide Magnetic Nanoparticles for Selective Drug Delivery to Human Acute Myeloid Leukemia

Cited by 13 publications

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Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

Advances in Magnetic Nanoparticles for Biomedical Applications

Fe₃O₄ @ PLGA‐PEG Nanocomposite for Improved Delivery of Methotrexate in Cancer Treatment

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Doxironide Magnetic Nanoparticles for Selective Drug Delivery to Human Acute Myeloid Leukemia

Cited by 13 publications

References 0 publications

Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

Preparation of iron oxide mesoporous magnetic microparticles as novel multidrug carriers for synergistic anticancer therapy and deep tumor penetration

Advances in Magnetic Nanoparticles for Biomedical Applications

Fe3O4 @ PLGA‐PEG Nanocomposite for Improved Delivery of Methotrexate in Cancer Treatment

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Fe₃O₄ @ PLGA‐PEG Nanocomposite for Improved Delivery of Methotrexate in Cancer Treatment