Draft Genome Sequence of Streptomyces sp. Strain JV178, a Producer of Clifednamide-Type Polycyclic Tetramate Macrolactams

Abstract

Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.

BAGEL4: a user-friendly web server to thoroughly mine RiPPs and bacteriocins

“…Alternatively the data can provide a starting point for (heterologous) production of novel ribosomally synthesized antimicrobial compounds ( 4 ). Also these servers have added value in annotation pipelines ( 5–7 ). Since the development of the first version of BAGEL ( 8 ) more web servers have been developed such as antiSMASH ( 9 ), PRISM ( 10 ) and recently RIPPMiner ( 11 ).…”

Draft Genome Sequences of Two Polycyclic Tetramate Macrolactam Producers, Streptomyces sp. Strains JV180 and SP18CM02

“…Recent advancements in genome sequencing have revealed Streptomyces as a reservoir of diverse biosynthetic gene clusters (BGCs). A high proportion of Streptomyces harbor BGCs encoding polycyclic tetramate macrolactams (PTMs), molecules of interest due to their structural complexity, atypical biosyntheses, and biomedical significance ( 2 – 4 ). Streptomyces sp.…”

The “Three Cs” of Novel Antibiotic Discovery and Production through Synthetic Biology: Biosynthetic Gene Clusters, Heterologous Chassis, and Synthetic Microbial Consortia

“…The combined use of PRISM3 with antiSMASH 4.0 by Qi et al recently identified 32 uninvestigated natural products from the genome of Streptomyces sp. strain JV178, including those in the bacteriocin, polyketide, and hybrid polyketide/nonribosomal peptide classes …”

“…strain JV178, including those in the bacteriocin, polyketide, and hybrid polyketide/nonribosomal peptide classes. [69] Other genome mining tools are used for the prediction of eukaryotic gene clusters, an example being SMURF (Secondary Metabolite Unique Regions Finder: http://www.jcvi.org/ smurf/index.php). [40] SMURF works similarly to fungiSMASH, utilizing rule-based alignment of domain structure with known fungal BGC enzymes and chromosomal position to determine BGCs for the production of PKSs, NRPSs, and terpenoids.…”