Draft Genome Sequence of Lactobacillus crispatus C25 Isolated from Chicken Cecum

Abstract

Lactic acid bacteria are important members of the gut microbiota of humans and animals. Here, we present the genome sequence of Lactobacillus crispatus strain C25, originally isolated from the cecum of 4-week-old chicken fed a standard diet. This isolate represents a potential probiotic strain for poultry.

Microbial and Functional Profile of the Ceca from Laying Hens Affected by Feeding Prebiotics, Probiotics, and Synbiotics

“…Olsenella spp., has been identified in the GIT of laying pullets being involved in lipid and cholesterol metabolism [25]. On the other hand, the observed increase of L. crispatus (OTU 3) could be considered desirable, because this phylotype has been classified as a beneficial probiotic, in poultry [26]. Due to its lactic acid production and competitive exclusion, supplementation of L. crispatus in broiler diets, reduces the colonization of Campylobacter jejuni and exerts an inhibitory effect against the presence of Salmonella enterica serovar Enteritidis [27].…”

Isolation, identification and characterization of probiotic properties of bacterium from the honey stomachs of Yigilca honeybees in Turkey

“…The molecular diagnostics with PCR amplification (Figure 2) revealed it has 99% 16S rRNA sequence similarity with the six strains of Lactobacillus kunkeei, tested and a phylogenetic tree was constructed using these reference bacteria (Figure 3). Among lactic acid bacteria, the genus Lactobacillus is one of the most important and includes about 174 species (Rezvani et al, 2016). Showing a wide distribution in different habitats, Lactobacillus species are located in the gastrointestinal tract of bees and many other animals (Mitsuoka, 1996;Schrezenmeir & de Vrese, 2001;Tannock, 2004;Fujisawa &Mitsuoka, 1996).…”

Complete Genome Sequences of Lactobacillus Strains C25 and P38, Isolated from Chicken Cecum

“…Previously, we published draft genomes of Lactobacillus crispatus strain C25 and Lactobacillus animalis strain P38, isolated from chickens ( 2 , 3 ). Strain C25 was isolated from pooled cecum samples from seven 4-week-old female Leghorn chickens grown on a commercial diet; strain P38 was also isolated from pooled cecum samples and from chickens grown under the same conditions as C25, except that the diet contained galactooligosaccharide as a prebiotic ( 4 ).…”

“…The original isolates were stored at −80°C in individual vials and never subcultured. Frozen cells were cultured in MRS medium and grown anaerobically for 20 h ( 2 , 3 ), and the DNA was extracted using the Promega Wizard genomic DNA purification kit. The DNA samples were sheared using a Covaris g-TUBE, and size selection was performed using BluePippin.…”

“…Strain C25 was isolated from pooled cecum samples from seven 4-week-old female Leghorn chickens grown on a commercial diet; strain P38 was also isolated from pooled cecum samples and from chickens grown under the same conditions as C25, except that the diet contained galactooligosaccharide as a prebiotic (4). The draft genomes were assembled from Illumina MiSeq reads (2,3). In this report, we resequenced strains C25 and P38 using PacBio to generate single-chromosome contigs.…”