Draft Genome Sequence of Talaromyces cellulolyticus Strain Y-94, a Source of Lignocellulosic Biomass-Degrading Enzymes

Abstract

Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) is a promising fungus for cellulase production. Here, we present the draft genome sequence of T. cellulolyticus strain Y-94. The genome is 36.4 Mbp long and contains genes for several enzymes involved in the degradation of lignocellulosic biomass, including cellulases, hemicellulases, pectinases, and amylases.

Effect of β-Mannanase and β-Mannosidase Supplementation on the Total Hydrolysis of Softwood Polysaccharides by the Talaromyces cellulolyticus Cellulase System

“…The mannan-degrading enzymes in TCM may be used as candidates to construct a suitable cellulase system for the hydrolysis of softwood using enzyme production technology or homologous recombination of Talaromyces cellulolyticus [18,33]. However, the [34]. Novel β-mannosidase appropriate for mannobiose hydrolysis may be detected by genome mining, and not only by the analysis of TCM.…”

Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain

“…Genomic DNA of T. cellulolyticus was sequenced, and the sequencing data have been registered in Genbank database. 10) Furthermore, efficient transformation and starch-inducible homologous expression systems have been developed, indicating that genetic engineering of T. cellulolyticus is already possible. 11,12) Furthermore, several gene products identified as regulators of cellulase and xylanase gene expression in T. cellulolyticus were found: CreA, carbon catabolite repressor; XlnR, inducer of xylanase genes; TacA and TctA, inducers of cellulase and xylanase genes.…”

Characterization of a feruloyl esterase B from Talaromyces cellulolyticus

“…We identified one putative feaB gene in the draft genome sequence of T. cellulolyticus 17) that exhibits some homology with carbohydrate esterases (EC: 3.1.1.73) using In silico Molecular Cloning gene analysis software (In Silico Biology, Inc., Yokohama, Japan) based on the internal amino acid sequence. The gene contains an open reading frame of 1071 bp and encodes a protein with molecular mass of 37,689 Da.…”

“…We identified the feruloyl esterase B homologous gene (faeB) by searching the draft genome sequence of T. cellulolyticus. 17) Here, we report the cloning, expression, and purification of the recombinant FaeB from T. cellulolyticus (TcFaeB) and its characterization.…”

“…19) Construction of the recombinant enzyme. A draft genome sequence of T. cellulolyticus 17) was searched for the feruloyl esterase gene (faeB) using Molecular Cloning gene analysis software (In Silico Biology, Inc., Yokohama, Japan) based on the internal amino acid sequence. Construction of the expression vector for T. cellulolyticus basically followed a procedure described previously.…”