Draft Genome Sequences for Five Strains of Trabulsiella odontotermitis, Isolated from
            <i>Heterotermes</i>
            sp. Termite Gut

Olvera-García, Myrna; Fontes-Perez, Héctor; Chávez‐Martínez, América; Ruiz-Barrera, Oscar; Rodríguez-Almeida, Felipe Alonso; Sánchez-Flores, Alejandro; Corral-Luna, Agustin

doi:10.1128/genomea.01289-15

Cited by 4 publications

(5 citation statements)

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“…isolated from the wood-feeding termite Heterotermes sp. showed the presence of an array of cellulolytic enzymes, suggesting their involvement in cellulose degradation within the digestive tract (Olvera-García et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

In Vivo Isotopic Labeling of Symbiotic Bacteria Involved in Cellulose Degradation and Nitrogen Recycling within the Gut of the Forest Cockchafer (Melolontha hippocastani)

et al. 2017

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The guts of insects harbor symbiotic bacterial communities. However, due to their complexity, it is challenging to relate a specific symbiotic phylotype to its corresponding function. In the present study, we focused on the forest cockchafer (Melolontha hippocastani), a phytophagous insect with a dual life cycle, consisting of a root-feeding larval stage and a leaf-feeding adult stage. By combining in vivo stable isotope probing (SIP) with 13C cellulose and 15N urea as trophic links, with Illumina MiSeq (Illumina-SIP), we unraveled bacterial networks processing recalcitrant dietary components and recycling nitrogenous waste. The bacterial communities behind these processes change between larval and adult stages. In 13C cellulose-fed insects, the bacterial families Lachnospiraceae and Enterobacteriaceae were isotopically labeled in larvae and adults, respectively. In 15N urea-fed insects, the genera Burkholderia and Parabacteroides were isotopically labeled in larvae and adults, respectively. Additionally, the PICRUSt-predicted metagenome suggested a possible ability to degrade hemicellulose and to produce amino acids of, respectively, 13C cellulose- and 15N urea labeled bacteria. The incorporation of 15N from ingested urea back into the insect body was confirmed, in larvae and adults, by isotope ratio mass spectrometry (IRMS). Besides highlighting key bacterial symbionts of the gut of M. hippocastani, this study provides example on how Illumina-SIP with multiple trophic links can be used to target microorganisms embracing different roles within an environment.

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Section: Discussionmentioning

confidence: 99%

In Vivo Isotopic Labeling of Symbiotic Bacteria Involved in Cellulose Degradation and Nitrogen Recycling within the Gut of the Forest Cockchafer (Melolontha hippocastani)

et al. 2017

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“…Suman et al [ 104 ] found the strain of IIPTG13 Trabulsiella also might play a role in the lignin degradation. Recently, Olvera-García et al [ 105 ] described the genome of five strains of Trabulsiella and found that this species has a pool of genes related to cellulose degradation. Maybe at the feed-out an aerobic microbial community capable of degrading cellulose settles down after the levels of soluble carbohydrates decrease.…”

Section: Discussionmentioning

confidence: 99%

Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

Gallo

Ghilardelli

Atzori

et al. 2021

Toxins

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Sixty-four corn silages were characterized for chemicals, bacterial community, and concentrations of several fungal metabolites. Silages were grouped in five clusters, based on detected mycotoxins, and they were characterized for being contaminated by (1) low levels of Aspergillus- and Penicillium-mycotoxins; (2) low levels of fumonisins and other Fusarium-mycotoxins; (3) high levels of Aspergillus-mycotoxins; (4) high levels of non-regulated Fusarium-mycotoxins; (5) high levels of fumonisins and their metabolites. Altersetin was detected in clusters 1, 3, and 5. Rugulusovin or brevianamide F were detected in several samples, with the highest concentration in cluster 3. Emodin was detected in more than 50.0% of samples of clusters 1, 3 and 5, respectively. Kojic acid occurred mainly in clusters 1 and 2 at very low concentrations. Regarding Fusarium mycotoxins, high occurrences were observed for FB3, FB4, FA1, whereas the average concentrations of FB6 and FA2 were lower than 12.4 µg/kg dry matter. Emerging Fusarium-produced mycotoxins, such as siccanol, moniliformin, equisetin, epiequisetin and bikaverin were detected in the majority of analyzed corn silages. Pestalotin, oxaline, phenopirrozin and questiomycin A were detected at high incidences. Concluding, this work highlighted that corn silages could be contaminated by a high number of regulated and emerging mycotoxins.

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“…In the present study, two genes were cloned from T. odontotermitis , and their expressed products (egl-FZYE and cel-FZYE), which exhibited endoglucanase and a β-glucosidase activity, respectively, were selected based on previous annotation of the T. odontotermitis genome [ 21 ]. The estimated masses of egl-FZYE and cel-FZYE were 42 and 55 kDa, respectively, which were similar to the values predicted by the ExPASy Bioinformatics Resources Portal [ 22 ] i.e., 41.5 and 55.4 kDa, respectively), whereas the estimated masses of the egl-FZYE and cel-FZYE fusion proteins were 70 and 83 kDa, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The sequences of the endoglucanase and ß-glucosidase genes were obtained from the genome annotation analyses performed for five T. odontotermitis strains isolated from the digestive tract of the termite Heterotermes sp. [ 21 ]. The coding sequences of both genes (omitting stop codons) were synthesized carrying the restriction sites for the NdeI and SapI enzymes at the 5′ and 3′ ends, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…However, relatively little is known about the specific identities and functions of species and genera found in the microflora of termite guts [ 19 , 20 ]. Indeed, Trabulsiella odontotermitis , which possesses an interesting set of genes related to cellulose degradation, with potential biotechnological applications, was only recently isolated and sequenced [ 21 ]. Accordingly, in order to harness the potential of this resource, the objective of the present study was to overexpress, purify, and characterize two recombinant cellulases from T. odontotermitis isolated from termite gut to evaluate their effects on the utilization of several types of agro-industrial waste.…”

Section: Introductionmentioning

confidence: 99%

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In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization

et al. 2021

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Background The production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly composed by cellulose. Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In the present experiment, two genes encoding an endoglucanase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) were overexpressed in Escherichia coli and their recombinant enzymes (egl-FZYE and cel-FZYE, respectively) characterized. Those genes were found in Trabulsiella odontermitis which was isolated from the gut of termite Heterotermes sp. Additionally, the capability to release sugars from agricultural wastes was evaluated in both enzymes, alone and in combination. Results The results have shown that optimal pH was 6.0 and 6.5, reaching an activity of 1051.65 ± 47.78 and 607.80 ± 10.19 U/mg at 39 °C, for egl-FZYE and cel-FZYE, respectively. The Km and Vmax for egl-FZYE using CMC as substrate were 11.25 mg/mL and 3921.57 U/mg, respectively, whereas using Avicel were 15.39 mg/mL and 2314.81 U/mg, respectively. The Km and Vmax for cel-FZYE using Avicel as substrate were 11.49 mg/mL and 2105.26 U/mg, respectively, whereas using CMC the enzyme did not had activity. Both enzymes had effect on agricultural wastes, and their effect was improved when they were combined reaching an activity of 955.1 ± 116.1, 4016.8 ± 332 and 1124.2 ± 241 U/mg on corn stover, sorghum stover and pine sawdust, respectively. Conclusions Both enzymes were capable of degrading agricultural wastes, and their effectiveness was improved up to 60% of glucose released when combined. In summary, the results of the study demonstrate that the recombinant enzymes exhibit characteristics that indicate their value as potential feed additives and that the enzymes could be used to enhance the degradation of cellulose in the poor-quality forage generally used in ruminant feedstuffs.

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Draft Genome Sequences for Five Strains of Trabulsiella odontotermitis, Isolated from Heterotermes sp. Termite Gut

Cited by 4 publications

References 10 publications

In Vivo Isotopic Labeling of Symbiotic Bacteria Involved in Cellulose Degradation and Nitrogen Recycling within the Gut of the Forest Cockchafer (Melolontha hippocastani)

In Vivo Isotopic Labeling of Symbiotic Bacteria Involved in Cellulose Degradation and Nitrogen Recycling within the Gut of the Forest Cockchafer (Melolontha hippocastani)

Co-Occurrence of Regulated and Emerging Mycotoxins in Corn Silage: Relationships with Fermentation Quality and Bacterial Communities

In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization

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