Draft Whole-Genome Sequence of Serratia sp. Strain TEL, Associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae) Isolated from a Grassland in South Africa

Abstract

Here, we report on the draft genome sequence of Serratia sp. strain TEL, associated with Oscheius sp. TEL-2014 (Nematoda: Rhabditidae, KM492926) isolated from a grassland in Suikerbosrand Nature Reserve near Johannesburg in South Africa. Serratia sp. strain TEL has a genome size of 5,000,541 bp with 4,647 genes and a G+C content of 59.1%.

Genome Sequences of Serratia Strains Revealed Common Genes in Both Serratomolides Gene Clusters

“…The swrA biosynthetic gene clusters were detected in strains belonging to the species S. marcescens (seven) , S. ureilytica (one), and three belong to unidentified Serratia species. These strains come from different sources, such as waste water [ 43 ], paper machines [ 34 ], infected patients [ 19 , 44 , 49 , 60 ], pond water, human tissue [ 35 ], rapeseed roots [ 29 , 30 , 31 ], nematodes, buffer solutions [ 25 ], different plants [ 39 , 61 ], and soil [ 27 , 28 ].…”

“…Eighty-four Serratia genomes available on NCBI, representing all the publicly available genomes, were included in this study ( Table S1 ), obtained from 49 different hosts and isolated from 29 countries [ 13 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ].…”

Whole genome sequence of Oscheius sp. TEL-2014 entomopathogenic nematodes isolated from South Africa

“…TEL-2014, which was found to be symbiotically associated with Serratia sp. TEL [13] , [14] . The infective juveniles of this entomopathogenic nematode were collected from freshly prepared White traps and surface sterilised with 0.1% sodium hypochlorite for 3 h in sterile 1.5 ml Eppendorf tubes.…”

Genome sequencing and annotation of Serratia sp. strain TEL

“…The whole genome was shotgun sequenced on the Illumina Miseq, following sequence quality control using Fastq-mcf toolkit and de novo assembly using SPADES were performed as described in [7] . A draft annotated genome of this organism was constructed using the best-placed reference protein set; GeneMarkS + annotation method set on the NCBI Prokaryotic Genome Automatic Annotation Pipeline and has been reported in [7] . Furthermore, the functional annotation was carried out by RAST (Rapid Annotation using Subsystem Technology) hosted by Fellowship for Interpretation.…”