Drought-mitigating Pseudomonas putida GAP-P45 modulates proline turnover and oxidative status in Arabidopsis thaliana under water stress

Ghosh, Daipayan; Sen, Sunetra; Mohapatra, Sridev

doi:10.1007/s13213-018-1366-7

Cited by 31 publications

(31 citation statements)

References 97 publications

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“…6A). Higher redox state in presence of P. putida under drought has also been reported by Ghosh 63 . Elevated H 2 O 2 in presence of RAR under control and salinity alone conditions supports the stress mitigation attribute of RAR to keep the nutrient availability high.…”

Section: Discussionsupporting

confidence: 73%

Prescience of endogenous regulation in Arabidopsis thaliana by Pseudomonas putida MTCC 5279 under phosphate starved salinity stress condition

Srivastava

2020

Sci Rep

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phosphorus (p) availability and salinity stress are two major constraints for agriculture productivity. A combination of salinity and p starvation is known to be more deleterious to plant health. plant growth promoting rhizobacteria are known to ameliorate abiotic stress in plants by increasing the availability of different nutrients. However, interaction mechanisms of plant grown under salinity and P stress condition and effect of beneficial microbe for stress alleviation is still obscure. Earlier we reported the molecular insight of auxin producing, phosphate solubilising Pseudomonas putida MTCC 5279 (RAR) mediated plant growth promotion in Arabidopsis thaliana. in present study new trait of proline and phosphatase production of RAR and its impact on modulation of physiological phenomenon under phosphate starved-salinity stress condition in A. thaliana has been investigated. Different physiological and molecular determinants under RAR-A. thaliana interaction showed that auxin producing RAR shows tryptophan dependence for growth and proline production in Atp dependant manner under salinity stress. However, under P deprived conditions growth and proline production are independent of tryptophan. RAR mediated lateral root branching and root hair density through modulation of abscisic acid signalling was observed. Acidic phosphatase activity under p starved and salinity stress condition was majorly modulated along with RoS metabolism and expression of stress responsive/phosphate transporter genes. A strong correlation of different morpho-physiological factor with RAR + salt conditions, showed We concluded that enhanced adverse effect of salinity with unavailability of P was dampened in presence of P. putida MTCC 5279 (RAR) in A. thaliana, though more efficiently salinity stress conditions. Therefore, alleviation of combined stress of salinity induced phosphate nutrient deficiency by inoculation of beneficial microbe, P. putida MTCC 5279 offer good opportunities for enhancing the agricultural productivity. Soil salinity, an important global problem occupies more than 7% of the earth's land surface. Approximately 7 million hectare of land in India is covered by saline soil with an yearly increase @ 0.3-1.5 million ha of farmland 1,2. It has been reported as most serious factor for limiting the productivity of agricultural crops by 20% 3,4. Numerous hostile effects such as osmotic stress, ion toxicity, hormonal imbalance and generation of reactive oxygen species are known to be induced by salinity stress in plants 5. These alterations are known to affect the growth and development of plant by impairing the nutritional balance. Among different nutrients, salinity stress are known to limit the phosphorus (P) availability, uptake and transport in plants probably due to its precipitation with other cations and hindrance of the acidic phosphatase activity 6-8. Phosphorus (P) is one of the major essential macronutrient, directly taken up by the plants in form of HPO 4 − ions. However, majority of it gets immobilized in forms...

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Section: Discussionsupporting

confidence: 73%

Prescience of endogenous regulation in Arabidopsis thaliana by Pseudomonas putida MTCC 5279 under phosphate starved salinity stress condition

Srivastava

2020

Sci Rep

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“…XT13 + Bacillus megaterium XT14 treatments with a decrease of 19.6 % and 24.2 % compared to that obtained with the non-immobilized organisms. These results corroborated those reported by Ghosh et al (2018) recording the highest level in all the redox (ROS molecules and antioxidant enzymes) under hydric stress; inoculation with Pseudomona putida GAP-P45 decreased ROS accumulation and all antioxidant enzyme activities including (APX) in seedlings of A. thaliana significantly ( P < 0.05) in the majority of the analysis points in time condition of water deficit. Based on these findings, we inferred that Guinea grass showed better growth under drought when PGPB were immobilized in macrobeads.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, we also report the impact of drought stress and PGPB inoculation on compatible osmolytes and activities of enzymatic antioxidants related to the redox status of Guinea grass. To accomplish this objective, proline accumulation and ascorbate peroxidase activity was assayed (Ghosh et al 2018). In such treatments we evidenced that proline decreased and APX increased significantly.…”

Section: Discussionmentioning

confidence: 99%

“…The relevance of these results is that they were obtained under drought stress using response variables, such as antioxidant enzymatic activity (APX), osmoprotector osmolite accumulation (proline), nutritional quality parameter (NDF, protein digestibility) and biomass production. Proline accumulation was assessed in this study because it plays many roles, such as preventing water exosmosis by decreasing cell water potential (Reddy et al 2015), acting as a source of energy (Szabados and Savouré 2010), maintaning cytosolic pH and intracellular redox potential (Ben Rejeb et al 2014), stabilizing protein structure through chaperon activity (Liang et al 2014) and preventing oxidative damage under abiotic stress conditions (Ghosh et al 2018). Likewise, APX is found in the chloroplasts, mitochondria, peroxisoma and cytosol detoxifying the hydrogen peroxide generated under drought conditions (Miller et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The bacteria of the genus Bacillus have an important role generating physiological and biochemical changes in plants (Yang et al 2009), providing induced systemic tolerance (IST) to these abiotic stress types (Khan et al 2019). The mechanisms related with IST include antioxidant defense (APX) (Ghosh et al 2018; Wang et al 2012), osmotic adjustment by accumulation of proline (Ghosh et al 2018), phytohormone production, such as indole-3-acetic acid (AIA) (Spaepen et al 2014) and defense strategies, such as expressions of genes related to pathogenesis (PR) (Kim et al 2012). At the same time, reports have demonstrated systemic tolerance induced by PGPB in cultivations under stress by drought, such as cucumber, corn, wheat, bean and chickpea in greenhouse conditions (Sandhya et al 2010; Wang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of drought tolerance on guinea grass by dry alginate macrobeads as inoculant of Bacillus strains

Mendoza-Labrador

Romero-Perdomo

Hernández³

et al. 2019

Preprint

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Drought is responsible for almost 70% of total crop damage in tropical and subtropical countries. In the Colombian Caribbean, drought has caused low availability of Guinea grass (Megathyrsus maximus) in forage amount and quality, generating increase in production costs in livestock systems. In this study, we aimed at designing and evaluating dry macro-polymeric inoculants of Bacillus strains and used them successfully to mitigate drought effect on Guinea grass (M. maximus). We chose Bacillus sp XT13 and Bacillus megaterium XT14 strains, previously selected for their capacity to mitigate drought stress on Guinea grass. For the inoculant formulation process, a concentration of 3.2% alginate was used for both microorganisms, selecting the polymer-bacterium ratio of 70:30 for XT13 and 80:20 for XT14. The results of the experiments in greenhouse showed that drought had a negative effect on Guinea grass growth, but the PGPB application with or without macro-polymeric dry matrix improved plant growth significantly. We also observed that co-inoculation of the strains generated a greater beneficial effect than individual inoculation. Interestingly, co-inoculation of XT13 and XT14 under the dry macro-polymeric formulation increased biomass production of the aerial part (7.32%), root biomass, (25.3%), nutritional quality parameters, such as digestibility (3.32%), protein (20.3%); proline accumulation increased (21.06%) and both neutral digestible fiber (2.43%) and APX antioxidant activity (24.2%) decreased with respect to co-inoculation of both bacteria without formulation. These findings have shown that dry macrobeads immobilization has a significantly positive influence on the capacity of XT13 and XT14 bacterial strains to induce drought stress tolerance in Guinea grass.

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GABA and Proline Metabolism in Response to Stress

Signorelli

Tarkowski

O’Leary

et al. 2021

Hormones and Plant Response

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Drought-mitigating Pseudomonas putida GAP-P45 modulates proline turnover and oxidative status in Arabidopsis thaliana under water stress

Cited by 31 publications

References 97 publications

Prescience of endogenous regulation in Arabidopsis thaliana by Pseudomonas putida MTCC 5279 under phosphate starved salinity stress condition

Prescience of endogenous regulation in Arabidopsis thaliana by Pseudomonas putida MTCC 5279 under phosphate starved salinity stress condition

Enhancement of drought tolerance on guinea grass by dry alginate macrobeads as inoculant of Bacillus strains

GABA and Proline Metabolism in Response to Stress

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