Drought Sensitivity of Sugarcane Cultivars Shapes Rhizosphere Bacterial Community Patterns in Response to Water Stress

Liu, Qi; Xie, Sasa; Zhao, Xue; Liu, Yue; Xing, Yuanjun; Dao, Jicao; Wei, Bo; Peng, Yunchang; WeiXing, Duan; Wang, Ziting

doi:10.3389/fmicb.2021.732989

Cited by 14 publications

(9 citation statements)

References 81 publications

(89 reference statements)

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“…However, a succession of studies have now suggested that this difference in drought tolerance levels between varieties (genotypes) is partly due to rhizosphere microbial differences . Studies on sugarcane (Liu Q. et al, 2021), tomato (Gaete et al, 2021), cotton (Ullah et al, 2019), soybean (Kuzmicheva et al, 2017), rice (Santos-Medellin et al, 2017), and switchgrass (Liu T. Y. et al, 2021) have all supported this view. Host plants are able to alter rhizosphere microbial composition by influencing root structure and secretions (Wen et al, 2020), which, in turn, enhances plant resistance through rhizosphere interactions, although this effect varies widely among species and genotypes.…”

Section: Introductionmentioning

confidence: 94%

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Wang

Tang²,

Wang³

et al. 2023

Front. Microbiol.

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Drought is a major abiotic stress that threatens crop production. Soil microbiomes are thought to play a role in enhancing plant adaptation to various stresses. However, it remains unclear whether soil microbiomes play a key role when plants are challenged by drought and whether different varieties are enriched with specific bacteria at the rhizosphere. In this study, we measured changes in growth phenotypes, physiological and biochemical characteristics of drought-tolerant alfalfa (AH) and drought-sensitive (QS) under sterilized and unsterilized soil conditions with adequate watering and with drought stress, and analyzed the rhizosphere bacterial community composition and changes using 16S rRNA high-throughput sequencing. We observed that the unsterilized treatment significantly improved the growth, and physiological and biochemical characteristics of alfalfa seedlings under drought stress compared to the sterilized treatment. Under drought stress, the fresh and dry weight of seedlings increased by 35.24, 29.04, and 11.64%, 2.74% for unsterilized AH and QS, respectively, compared to sterilized treatments. The improvement was greater for AH than for QS. AH and QS recruited different rhizosphere bacteria when challenged by drought. Interestingly, under well-watered conditions, the AH rhizosphere was already rich in drought-tolerant bacterial communities, mainly Proteobacteria and Bacteroidetes, whereas these bacteria started to increase only when QS was subjected to drought. When drought stress was applied, AH was enriched with more drought-tolerant bacteria, mainly Acidobacteria, while the enrichment was weaker in QS rhizosphere. Therefore, the increase in drought tolerance of the drought-tolerant variety AH was greater than that of the drought-sensitive variety QS. Overall, this study confirmed the key role of drought-induced rhizosphere bacteria in improving the adaptation of alfalfa to drought stress, and clarified that this process is significantly related to the variety (genotype). The results of this study provide a basis for improving drought tolerance in alfalfa by regulating the rhizosphere microbiome.

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Section: Introductionmentioning

confidence: 94%

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Wang

Tang²,

Wang³

et al. 2023

Front. Microbiol.

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“…It has previously been reported that different components of soil microbial communities respond differently to drought. A previous study on sugarcane-root-associated bacteria reported that drought stress significantly reduced the overall bacterial diversity while only increasing the abundance of drought-resistant bacteria in the sugarcane rhizosphere (Liu et al, 2021). Soil bacterial networks are known to be less stable than fungal networks under drought stress.…”

Section: Fungal Communities Were Less Impacted By Peg6000 Treatment T...mentioning

confidence: 97%

“…The phylum Actinobacteria is often specifically enriched in relatively dry environments or under drought conditions (Xu et al, 2018;Kumar et al, 2022). Actinobacteria was reported to be crucial for a drought-sensitive sugarcane cultivar GT39 to cope with drought stress, while a relatively drought-resistant sugarcane cultivar ZZ9 relied mainly on Bacilli to adapt to drought (Liu et al, 2021). A study looking at rice root-associated microbiota found that changes in bacterial communities in response to drought were taxonomically consistent across soils and different rice cultivars and were primarily driven by the enrichment of the genera Actinobacteria and Chloroflexi and, depletion of several Deltaproteobacteria species (Santos-Medellıń et al, 2017).…”

Section: Drought Stress Can Impact a Lancea Growth And Medicinal Comp...mentioning

confidence: 99%

Drought stress modifies the community structure of root-associated microbes that improve Atractylodes lancea growth and medicinal compound accumulation

Wang

Kang

et al. 2022

Front. Plant Sci.

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Atractylodes lancea is an important medicinal plant in traditional Chinese medicine, its rhizome is rich of volatile secondary metabolites with medicinal values and is largely demanded in modern markets. Currently, supply of high-yield, high-quality A. lancea is mainly achieved via cultivation. Certain soil microbes can benefit plant growth, secondary metabolism and induce resistance to environmental stresses. Hence, studies on the effects of soil microbe communities and isolates microorganisms on A. lancea is extremely meaningful for future application of microbes on cultivation. Here we investigated the effects of the inoculation with an entire soil microbial community on the growth, resistance to drought, and accumulation of major medicinal compounds (hinesol, β-eudesmol, atractylon and atractylodin) of A. lancea. We analyzed the interaction between A. lancea and the soil microbes at the phylum and genus levels under drought stress of different severities (inflicted by 0%, 10% and 25% PEG6000 treatments). Our results showed that inoculation with soil microbes promoted the growth, root biomass yield, medicinal compound accumulation, and rendered drought-resistant traits of A. lancea, including relatively high root:shoot ratio and high root water content under drought. Moreover, our results suggested drought stress was more powerful than the selectivity of A. lancea in shaping the root-associated microbial communities; also, the fungal communities had a stronger role than the bacterial communities in protecting A. lancea from drought. Specific microbial clades that might have a role in protecting A. lancea from drought stress were identified: at the genus level, the rhizospheric bacteria Bacillus, Dylla and Actinomadura, and rhizospheric fungi Chaetomium, Acrophialophora, Trichoderma and Thielava, the root endophytic bacteria Burkholderia-Caballeronia-Paraburkholderia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Dylla and Actinomadura, and the root endophytic fungus Fusarium were closely associated with A. lancea under drought stress. Additionally, we acquired several endophytic Paenibacillus, Paraburkholderia and Fusarium strains and verified they had differential promoting effects on the medicinal compound accumulation in A. lancea root. This study reports the interaction between A. lancea and soil microbe communities under drought stress, and provides insights for improving the outcomes in A. lancea farming via applying microbe inoculation.

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“…Sugarcane ( Saccharum spp.) is an important sugar and energy crop that has strict water requirements for cultivation [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Co-Expression Analysis Reveals Transcriptome Divergence between Wild and Cultivated Sugarcane under Drought Stress

Lin

Zhong

et al. 2022

IJMS

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Drought is the main abiotic stress that constrains sugarcane growth and production. To understand the molecular mechanisms that govern drought stress, we performed a comprehensive comparative analysis of physiological changes and transcriptome dynamics related to drought stress of highly drought-resistant (ROC22, cultivated genotype) and weakly drought-resistant (Badila, wild genotype) sugarcane, in a time-course experiment (0 h, 4 h, 8 h, 16 h and 32 h). Physiological examination reviewed that ROC22, which shows superior drought tolerance relative to Badila, has high performance photosynthesis and better anti-oxidation defenses under drought conditions. The time series dataset enabled the identification of important hubs and connections of gene expression networks. We identified 36,956 differentially expressed genes (DEGs) in response to drought stress. Of these, 15,871 DEGs were shared by the two genotypes, and 16,662 and 4423 DEGs were unique to ROC22 and Badila, respectively. Abscisic acid (ABA)-activated signaling pathway, response to water deprivation, response to salt stress and photosynthesis-related processes showed significant enrichment in the two genotypes under drought stress. At 4 h of drought stress, ROC22 had earlier stress signal transduction and specific up-regulation of the processes response to ABA, L-proline biosynthesis and MAPK signaling pathway–plant than Badila. WGCNA analysis used to compile a gene regulatory network for ROC22 and Badila leaves exposed to drought stress revealed important candidate genes, including several classical transcription factors: NAC87, JAMYB, bHLH84, NAC21/22, HOX24 and MYB102, which are related to some antioxidants and trehalose, and other genes. These results provide new insights and resources for future research and cultivation of drought-tolerant sugarcane varieties.

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Drought Sensitivity of Sugarcane Cultivars Shapes Rhizosphere Bacterial Community Patterns in Response to Water Stress

Cited by 14 publications

References 81 publications

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Drought-induced recruitment of specific root-associated bacteria enhances adaptation of alfalfa to drought stress

Drought stress modifies the community structure of root-associated microbes that improve Atractylodes lancea growth and medicinal compound accumulation

Gene Co-Expression Analysis Reveals Transcriptome Divergence between Wild and Cultivated Sugarcane under Drought Stress

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