Drug Delivery and Imaging with Polydiacetylene Micelles

Gravel, Edmond; Ogier, Julien; Arnauld, Thomas; Mackiewicz, Nicolas; Ducongé, Frédéric; Doris, Eric

doi:10.1002/chem.201102769

Cited by 88 publications

(67 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Polydiacetylenes (PDAs)1819202122232425262728 possess unique structural features that differ from those of other conjugated polymers. As they are prepared by polymerization of self-assembled diacetylene monomers (Fig.…”

mentioning

confidence: 99%

Hydrochromic conjugated polymers for human sweat pore mapping

et al. 2014

View full text Add to dashboard Cite

Hydrochromic materials have been actively investigated in the context of humidity sensing and measuring water contents in organic solvents. Here we report a sensor system that undergoes a brilliant blue-to-red colour transition as well as ‘Turn-On’ fluorescence upon exposure to water. Introduction of a hygroscopic element into a supramolecularly assembled polydiacetylene results in a hydrochromic conjugated polymer that is rapidly responsive (<20 μs), spin-coatable and inkjet-compatible. Importantly, the hydrochromic sensor is found to be suitable for mapping human sweat pores. The exceedingly small quantities (sub-nanolitre) of water secreted from sweat pores are sufficient to promote an instantaneous colorimetric transition of the polymer. As a result, the sensor can be used to construct a precise map of active sweat pores on fingertips. The sensor technology, developed in this study, has the potential of serving as new method for fingerprint analysis and for the clinical diagnosis of malfunctioning sweat pores.

show abstract

mentioning

confidence: 99%

Hydrochromic conjugated polymers for human sweat pore mapping

et al. 2014

View full text Add to dashboard Cite

show abstract

“…To address the toxicity issues that NPs of various chemistries might exert, PDA liposomes were developed and used for a variety of purposes, including for oral delivery of various compounds. The polydiacetylene micelles were shown to have little or no toxicity, although, it was not shown specifically that micelles are biodegradable . The biodegradability of our liposomes depends upon composition of lipids.…”

Section: Resultsmentioning

confidence: 81%

Immunogenicity of antigen‐conjugated biodegradable polydiacetylene liposomes administered mucosally

Abrams

Howe

Becerra

et al. 2016

J Biomedical Materials Res

View full text Add to dashboard Cite

In this work, we report a protocol for synthesizing nanosize ovalbumin-functionalized polydiacetylene (PDA) liposomes (LP-Ova). We show that LP-Ova administered per-orally (p.o.) and subcutaneously (s.c.), without the use of adjuvants, induces high serum IgG1 titers. As reported previously using polystyrene nanoparticles (NPs), p.o.-primed mice developed high titers of IgG2c and intestinal IgA following s.c. boosting immunization with LP-Ova. Mice that received a single s.c. immunization with LP-Ova did not develop serum IgG2c or intestinal IgA antibodies. Additionally, in s.c.-immunized mice serum IgG1 titers decreased significantly by 3 months after immunization. In contrast, in mice primed p.o. and boosted s.c. with LP-Ova, serum IgG1/IgG2c, and intestinal IgA antibody titers remained stable. Administration of LPs exerted no adverse effects on immunized mice as no morbidity or signs of toxicity were observed for the duration of the studies. These results indicate that antigen-conjugated liposomes are immunogenic and confirm a previous report that mucosal priming followed by a s.c. boosting immunization is the most effective strategy for inducing long-lasting mucosal IgA, as well as a polarized Th1/Th2 systemic response. In addition to being biodegradable and easily functionalized by conjugation, liposomes have a hollow core which can also be loaded with cargo, allowing for a targeted delivery of multiple antigens (or drugs) simultaneously. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 557-565, 2017.

show abstract

“…Photopolymerization of DC was merely obtained when extending the irradiation time to several hours, forming PDC in pale yellow. The discriminative photopolymerization between DA and DC is ascribed to that the bilayer arrangement of DA vesicle is much more favorable for photopolymerization than the curvature arrangement of DC micelle 43, 44, 45, 46…”

Section: Resultsmentioning

confidence: 99%

Sequentially Programmable and Cellularly Selective Assembly of Fluorescent Polymerized Vesicles for Monitoring Cell Apoptosis

et al. 2017

View full text Add to dashboard Cite

The introduction of controlled self‐assembly into living organisms opens up desired biomedical applications in wide areas including bioimaging/assays, drug delivery, and tissue engineering. Besides the enzyme‐activated examples reported before, controlled self‐assembly under integrated stimuli, especially in the form of sequential input, is unprecedented and ultimately challenging. This study reports a programmable self‐assembling strategy in living cells under sequentially integrated control of both endogenous and exogenous stimuli. Fluorescent polymerized vesicles are constructed by using cholinesterase conversion followed by photopolymerization and thermochromism. Furthermore, as a proof‐of‐principle application, the cell apoptosis involved in the overexpression of cholinesterase in virtue of the generated fluorescence is monitored, showing potential in screening apoptosis‐inducing drugs. The approach exhibits multiple advantages for bioimaging in living cells, including specificity to cholinesterase, red emission, wash free, high signal‐to‐noise ratio.

show abstract

Drug Delivery and Imaging with Polydiacetylene Micelles

Cited by 88 publications

References 18 publications

Hydrochromic conjugated polymers for human sweat pore mapping

Hydrochromic conjugated polymers for human sweat pore mapping

Immunogenicity of antigen‐conjugated biodegradable polydiacetylene liposomes administered mucosally

Sequentially Programmable and Cellularly Selective Assembly of Fluorescent Polymerized Vesicles for Monitoring Cell Apoptosis

Contact Info

Product

Resources

About