Drug loaded microbeads entrapped electrospun mat for wound dressing application

“…The hemolysis rate and platelet adhesion rate indicated that MbAPPCL had blood compatibility. A susceptibility test of MbAPPCL to Staphylococcus aureus also showed its excellent antibacterial properties 128.…”

mentioning

confidence: 98%

Electrospun fibers and their application in drug controlled release, biological dressings, tissue repair, and enzyme immobilization

et al. 2019

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“…Better strategy is to coat a candidate barrier material with Lys‐PDA so that the surface gets functionalized . Previously standardized procedures were utilized to synthesize nanofibrous PCL membrane resembling the structural component of ECM . PCL is an FDA approved, biodegradable linear polyester, which can be tuned to improve surface properties to suit the biological needs .…”

Section: Discussionmentioning

confidence: 99%

“…28 Previously standardized procedures were utilized to synthesize nanofibrous PCL membrane resembling the structural component of ECM. 28,40 PCL is an FDA approved, biodegradable linear polyester, which can be tuned to improve surface properties to suit the biological needs. 41 Peptide functionalization can be utilized to impart cell adhesive properties to the PCL mats.…”

Section: Discussionmentioning

confidence: 99%

Self‐assembling polymeric dendritic peptide as functional osteogenic matrix for periodontal regeneration scaffolds—an in vitro study

Das

Dhawan

Babu

et al. 2019

J of Periodontal Research

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Objective Regeneration of periodontal defects is challenging as it necessitates the formation of complex tissue structure with cementum, periodontal ligament, and alveolar bone. Rather than the conventional barrier membranes, scaffolds mimicking extracellular matrix (ECM) can achieve faster healing as they promote migration, adhesion, and differentiation of native progenitor cells. This work explores the possibility of a functional osteogenic matrix based on self‐assembling peptide appended dendritic polydiacetylene in regenerating diseased periodontia. Method The amino acid lysine was appended onto a diacetylene core, which was converted to a polymeric dendritic lysine matrix (Lys‐PDA) through photopolymerization. This bioactive matrix was evaluated in vitro for the viability, adhesion, spreading, and differentiation of cultured human periodontal ligament (hPDL) progenitor cells. Its osteogenic differentiation was analysed by histologic staining and expression of osteogenic markers (alkaline phosphatase and Osteonectin). Electrospun polycaprolactone (PCL) mat, a candidate barrier material, was fabricated and functionalized with Lys‐PDA matrix, and the cell viability, adhesion, and spreading of hPDL cells were evaluated. Results The dendritic Lys‐PDA matrix well supported the hPDL cell growth and differentiation. The cells were viable and showed good cytoskeletal organization. Early expression of osteogenic markers and mineralization was noted in vitro in the presence of Lys‐PDA matrix. The electrospun PCL mat functionalized with Lys‐PDA maintained the viability, morphology, and spreading of the hPDL cells. Significance The ECM mimetic dendritic peptide matrices are capable of hosting and differentiating cells which can lead to the regeneration of periodontal tissue architecture. They could be used in conjunction with barrier membranes for better results.

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“…9 A 10% PCL (average Mn 80000, Sigma) solution was prepared using a binary solvent system of Terahydrofuran and Dimethyl sulphoxide. The polymer was taken in a syringe with a blunt end needle and connected to a customized electrospinning setup (Holmarc Opto-Mechatronics Pvt Ltd, India).…”

Section: Fabrication Of Electrospun Matsmentioning

confidence: 99%

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

Kumar¹

2017

ATROA

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Tissue engineered skin substitutes are used in chronic, partial or full thickness wounds, and serve to augment the growth and repair of the skin at the wound site, by acting as a temporary or permanent skin replacement. In this study Polycaprolactone a biodegradable polymer was electrospun in a binary solvent system to form nonwoven mats with porous fibers (PPCL). These mats were hydrolysed with alkali to make them more hydrophilic and promote cell adhesion. The electrospun mat served as a scaffold for culture of human keratinocyte (HaCaT) and rabbit hair follicle dermal fibroblast cells (HFDF) cells. F-actin staining and SEM analysis showed that the cells exhibited their characteristic morphology, with good adhesion and spreading. Live dead staining confirmed the viability of the cells on the PPCL mat. A bioengineered construct of HaCaT and HFDF cells cultured on either side of the PPCL mat was generated in vitro. Light microscopy of Toluidine blue stained cryo sections of the construct reveal that the HaCaT cells formed intact multilayers, while the fibroblasts showed a more distributed growth pattern with migration into the mat, hence emulating the epidermis and dermis of the skin. The results were substantiated in the SEM micrographs and fluorescent images of DiI and DiO labeled cocultures. The PPCL mat cocultured with HaCaT and HFDF cells thus finds potential as a self organized tissue engineered skin substitute..

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Drug loaded microbeads entrapped electrospun mat for wound dressing application

Cited by 8 publications

References 35 publications

Electrospun fibers and their application in drug controlled release, biological dressings, tissue repair, and enzyme immobilization

Electrospun fibers and their application in drug controlled release, biological dressings, tissue repair, and enzyme immobilization

Self‐assembling polymeric dendritic peptide as functional osteogenic matrix for periodontal regeneration scaffolds—an in vitro study

Self Organized Skin Equivalent by Epithelial and Fibroblast Cells on Polycaprolactone Electrospun Mat with Porous Fibers

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