Drug Repurposing: In vitro and in vivo Antimicrobial and Antibiofilm Effects of Bithionol Against Enterococcus faecalis and Enterococcus faecium

She, Pengfei; Wang, Yangxia; Li, Yingjia; Zhou, Linying; Li, Shijia; Zeng, Xianghai; Liu, Yaqian; Xu, Lei; Wu, Yong

doi:10.3389/fmicb.2021.579806

Cited by 14 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While statements were occasionally included to imply that the experiments were controlled to a degree, greater transparency regarding the control conditions and any findings relative to the treatments would be best. It was also problematic when DMSO concentrations in treatments varied and a single control concentration was used [ 41 , 89 ], or when the DMSO exposure and control concentrations did not match [ 46 ]. Moving forward, DMSO control concentrations should reflect all DMSO concentrations that biofilms are exposed to by means of delivering the extract/compound of interest.…”

Section: Resultsmentioning

confidence: 99%

Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Summer

Browne

Hollanders

et al. 2022

Biofilm

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Summer

Browne

Hollanders

et al. 2022

Biofilm

View full text Add to dashboard Cite

“…FICI was calculated using the formula: FICI = FIC A + FIC B ; FIC A = MIC A in combination/MIC A alone; FIC B = MIC B in combination/MIC B alone. The interactions were explained as follows: FICI ≤0.5, Synergistic effect; 0.5< FICI ≤4, No interaction; Antagonism of FICI >4 ( She et al, 2021 ). All experiments were conducted in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo

et al. 2022

View full text Add to dashboard Cite

The global emergence and spread of multi-drug resistant (MDR) strains is becoming increasingly worrisome due to the overuse of broad-spectrum antibiotics. Colistin, the last resort for treating MDR strains infections, has once again returned to the clinician’s choice. However, with the widespread use of colistin, colistin-resistant gram-negative bacteria (GNB) have subsequently emerged, including colistin-resistant Pseudomonas aeruginosa (COL-R PA). Therefore, available solutions are urgently needed to respond to this situation. Here, we inspiringly found that the combination of plumbagin and colistin had an efficiently inhibitory effect for colistin-resistant P. aeruginosa in vitro through checkerboard assay and time-kill assay. The combinatorial inhibition of biofilm formation was clearly demonstrated by crystal violet staining and scanning electron microscopy (SEM), and this combination can not only inhibited biofilm formation but also eradicated the mature biofilm. Erythrocytes hemolysis test showed that plumbagin has negligible hemolysis ability. In addition, the increased survival rate of Galleria mellonella (G. mellonella) larva confirmed this combination as same as effective in vivo. As for the mechanism of this combination, propidium iodide (PI) staining showed colistin combined with plumbagin could significantly change the membrane permeability, thus exerting synergistic antibacterial activity. In conclusion, the combination of plumbagin and colistin shows a prominently synergistic antibacterial effect in vitro and in vivo, providing a promising option for the therapy of COL-R PA infection.

show abstract

“…The FICI was calculated using the formula: FICI = (MIC of drug A in the combination/MIC of drug A alone) + (MIC of drug B in the combination/MIC of drug B alone). Interactions were interpreted as follows: synergy for FICI ≤ 0.5; no interaction for 0.5 < FICI ≤ 4; antagonism for FICI > 4 (She et al, 2021). Each test was performed in triplicate.…”

Section: Checkerboard Assaysmentioning

confidence: 99%

Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo

et al. 2021

View full text Add to dashboard Cite

Colistin is a polymyxin antibiotic that is widely used for the treatment of multidrug resistant (MDR) Pseudomonas aeruginosa infections, as the last resort. Over the past few years, unreasonable use of antibiotics has resulted in an increase in MDR strains, including colistin-resistant P. aeruginosa. The present study aimed to explore the synergistic effects of mefloquine in combination with colistin for the treatment of colistin-resistant P. aeruginosa in vivo and in vitro. The synergistic effect of the combination of mefloquine and colistin was investigated in vitro using checkerboard method, time-killing assay, biofilm formation inhibition test, and biofilm eradication test. The study also explored the synergistic effects of this combination of drugs in vivo, using a Galleria mellonella infection model. The results for checkerboard method and time killing curve indicated that mefloquine in combination with colistin showed a good antibacterial activity. Furthermore, the combination of these two drugs inhibited biofilm formation and eradicated pre-formed mature biofilms. This synergistic effect was visualized using scanning electron microscopy (SEM), wherein the results showed that the combination of mefloquine and colistin reduced biofilm formation significantly. Further, the application of this combination of drugs to in vivo infection model significantly increased the survival rate of G. mellonella larvae. Altogether, the combination of mefloquine and colistin showed a good synergistic effect in vitro and in vivo, and highlighted its potential to be used as an alternative therapy for the treatment of colistin-resistant P. aeruginosa infection.

show abstract

Drug Repurposing: In vitro and in vivo Antimicrobial and Antibiofilm Effects of Bithionol Against Enterococcus faecalis and Enterococcus faecium

Cited by 14 publications

References 27 publications

Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo

Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo

Contact Info

Product

Resources

About