Dual colorimetric and luminescent assay for dipicolinate, a biomarker of bacterial spores

Abstract

A binary mixture of Tb3+ and pyrocatechol violet (PV) forms a 1:1 Tb3+/PV complex that can be used in a dye displacement assay. Addition of dipicolinate (DPA) to the Tb3+/DPA complex simultaneously produces a PV color change from blue to yellow and luminescence emission from the newly formed Tb3+/DPA complex.

BSA–AuNPs@Tb–AMP metal–organic frameworks for ratiometric fluorescence detection of DPA and Hg2+

“…Based on the signal to noise ratio of 3, the detection limits for Hg 2+ and DPA were calculated to be 20.9 nM and 17.4 nM, respectively. As shown in Tables S1 and S2, the designed probe displayed excellent properties as compared with other DPA and Hg 2+ sensors . Such good performances might be ascribed to the more efficient use of the surface area of the core‐shell structure of BSA–AuNPs@Tb–AMP MOFs and the self‐calibration of the concentration from ratiometric fluorescence measurement …”

“…sensors. [28,[39][40][41][42][43][44][45][46][47][48][49] Such good performances might be ascribed to the more efficient use of the surface area of the core-shell structure of BSA-AuNPs@Tb-AMP MOFs and the self-calibration of the concentration from ratiometric fluorescence measurement. [29] Furthermore, some possibly coexisted interfering substances were also tested, such as metal ions (Na + , K + , Mg 2+ , Ca 2+ , Ni 2+ , Zn 2+ , NH 4+ , Fe 2+ , Cd 2+ ) ( Figure 6b) and organic molecules (p-phthalic acid, benzoic acid, trimestic acid, L-asparaginic acid, L-glutamic acid, L-cysteine acid) ( Figure 6a).…”

“…It is really worth pursuing high sensitivity methods for Hg 2+ detection because Hg 2+ is non‐biodegradable and bioaccumulables through the food chain and therefore could damage the brain, kidneys, and the nervous system . Conversely, DPA detection is also of great importance because it is the major components in protective layers of Bacillus anthracis spores . Bacillus anthracis spores, which have resulted in large numbers of population deaths throughout history, can be released into bulk solution by physical hydrolysis, chemical hydrolysis or germination and their mass can be calculated using their DPA concentration .…”

“…[24] Conversely, DPA detection is also of great importance because it is the major components in protective layers of Bacillus anthracis spores. [25][26][27][28][29][30][31] Bacillus anthracis spores, which have resulted in large numbers of population deaths throughout history, can be released into bulk solution by physical hydrolysis, chemical hydrolysis or germination and their mass can be calculated using their DPA concentration. [32,33] This compound provides prerequisites for simultaneously detection of bacterial spores and Hg 2+ for use in water quality monitoring.…”

Eu(III)–DO3A and BODIPY dyad as a chemosensor for anthrax biomarker

“…Assorted chemical and biological techniques including high‐performance liquid chromatography (HPLC), [ 43 ] surface‐enhanced Raman scattering, [ 44 ] luminescence detection, [ 40,45–53 ] electrochemical detection, [ 54 ] immunoassays, [ 34 ] and polymerase chain reaction (PCR), [ 55 ] can be used for the determination of bacterial spores. In particular, lanthanide luminescence has become a powerful tool for sensing DPA due to its selectivity, sensitivity, straightforward and quick detection, and low cost [for europium (Eu) based sensors see references [ 40,48,50,51,56–72 ] ; for other lanthanide based DPA sensors see references [ 41,45,47,54,73–87 ] ; for other DPA sensors see references. [ 88–92 ]…”