Dual-Drug Delivery via Zein In Situ Forming Implants Augmented with Titanium-Doped Bioactive Glass for Bone Regeneration: Preparation, In Vitro Characterization, and In Vivo Evaluation

Eldeeb, Alaa Emad; Salah, Samia; Mabrouk, Mostafa; Amer, Mona G.; Elkasabgy, Nermeen A.

doi:10.3390/pharmaceutics14020274

Cited by 16 publications

(14 citation statements)

References 101 publications

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“…The STF was prepared by dissolving NaCl (3.4 g), NaHCO 3 (1.1 g), KCl (0.7 g), and CaCl 2 ·2H 2 O (0.04 g) in 500 mL of Milli-Q ® water. A dialysis bag was used as a release barrier [ 41 ]. Around 1 mL of F2 suspension (~821.5 µg of TZP) was put into the dialysis bags, and both ends of the bags were tied with threads.…”

Section: Methodsmentioning

confidence: 99%

Development and Evaluation of Chitosan Nanoparticles for Ocular Delivery of Tedizolid Phosphate

et al. 2022

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This study investigates the development of topically applied non-invasive chitosan-nanoparticles (CSNPs) for ocular delivery of tedizolid phosphate (TZP) for the treatment of MRSA-related ocular and orbital infections. An ionic-gelation method was used to prepare TZP-encapsulated CSNPs using tripolyphosphate-sodium (TPP) as cross-linker. Particle characterization was performed by the DLS technique (Zeta-Sizer), structural morphology was observed by SEM. The drug encapsulation and loading were determined by the indirect method. In-vitro release was conducted through dialysis bags in simulated tear fluid (pH 7) with 0.25% Tween-80. Physicochemical characterizations were performed for ocular suitability of CSNPS. An antimicrobial assay was conducted on different strains of Gram-positive bacteria. Eye-irritation from CSNPs was checked in rabbits. Transcorneal flux and apparent permeability of TZP from CSNPs was estimated through excised rabbit cornea. Ionic interaction between the anionic and cationic functional groups of TPP and CS, respectively, resulted in the formation of CSNPs at varying weight ratios of CS/TPP with magnetic stirring (700 rpm) for 4 h. The CS/TPP weight ratio of 3.11:1 with 10 mg of TZP resulted in optimal-sized CSNPs (129.13 nm) with high encapsulation (82%) and better drug loading (7%). Release profiles indicated 82% of the drug was released from the TZP aqueous suspension (TZP-AqS) within 1 h, while it took 12 h from F2 to release 78% of the drug. Sustained release of TZP from F2 was confirmed by applying different release kinetics models. Linearity in the profile (suggested by Higuchi’s model) indicated the sustained release property CSNPs. F2 has shown significantly increased (p < 0.05) antibacterial activity against some Gram-positive strains including one MRSA strain (SA-6538). F2 exhibited a 2.4-fold increased transcorneal flux and apparent permeation of TZP as compared to TZP-AqS, indicating the better corneal retention. No sign or symptoms of discomfort in the rabbits’ eyes were noted during the irritation test with F2 and blank CSNPs, indicating the non-irritant property of the TZP-CSNPs. Thus, the TZP-loaded CSNPs have strong potential for topical use in the treatment of ocular MRSA infections and related inflammatory conditions.

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Section: Methodsmentioning

confidence: 99%

Development and Evaluation of Chitosan Nanoparticles for Ocular Delivery of Tedizolid Phosphate

et al. 2022

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“…Though it lacks a cell proliferative effect, so blending zein with bioactive substances (e.g. bioceramics, calcium phosphates, and osteoinductive drugs) is preferred to support the proliferation and differentiation of bone cells [ 82 , 89 , 90 ].…”

Section: Biomaterials In Tissue Engineeringmentioning

confidence: 99%

“…The fabricated implant showed a sustained release of both drugs for 28 days. In vivo studies on Sprague Dawley rats demonstrated a significant bone regenerating effect [ 82 ]. This study succeeded to introduce zein as a promising implant matrix; however, more studies and investigations should be conducted to optimize the fabricated implant to be applicable for large-sized bone defects.…”

Section: Biomaterials In Tissue Engineeringmentioning

confidence: 99%

“…Titanium (Ti) is widely used in bone implants due to several reasons; (i) Ti implants are considered the ideal choice for hard tissue replacement as they possess mechanical properties resembling those of natural bone tissues [ 165 ]; (ii) it can create a permanent bond to the bone via osseointegration; (iii) it enhances cell attachment and proliferation; (iv) it improves the mechanical properties of the scaffold by their characteristic load-bearing support ability [ 166 ]; and (v) it has been reported to exert an antibacterial effect to the implant [ 82 , 167 ].…”

Section: Biomaterials In Tissue Engineeringmentioning

confidence: 99%

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Biomaterials for Tissue Engineering Applications and Current Updates in the Field: A Comprehensive Review

2022

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Tissue engineering has emerged as an interesting field nowadays; it focuses on accelerating the auto-healing mechanism of tissues rather than organ transplantation. It involves implanting an In Vitro cultured initiative tissue or a scaffold loaded with tissue regenerating ingredients at the damaged area. Both techniques are based on the use of biodegradable, biocompatible polymers as scaffolding materials which are either derived from natural (e.g. alginates, celluloses, and zein) or synthetic sources (e.g. PLGA, PCL, and PLA). This review discusses in detail the recent applications of different biomaterials in tissue engineering highlighting the targeted tissues besides the in vitro and in vivo key findings. As well, smart biomaterials (e.g. chitosan) are fascinating candidates in the field as they are capable of elucidating a chemical or physical transformation as response to external stimuli (e.g. temperature, pH, magnetic or electric fields). Recent trends in tissue engineering are summarized in this review highlighting the use of stem cells, 3D printing techniques, and the most recent 4D printing approach which relies on the use of smart biomaterials to produce a dynamic scaffold resembling the natural tissue. Furthermore, the application of advanced tissue engineering techniques provides hope for the researchers to recognize COVID-19/host interaction, also, it presents a promising solution to rejuvenate the destroyed lung tissues. Graphical abstract

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“…Pediatrics, geriatrics, and disabled patients also require special treatment to enhance their adherence to their therapy. Implantable drug delivery systems could introduce a good solution (Abdel-Salam et al., 2020 ; Kamel et al., 2020 ; Eldeeb et al., 2022 ) to avoid fluctuating drug levels and sustain its release, hence increasing therapeutic effect and patient compliance (Ainslie & Desai, 2008 ; Elkasabgy & Mahmoud, 2019 ). Transdermal drug delivery systems, such as microneedles are designed to deliver the skin through the bloodstream (Alkilani et al., 2015 ).…”

Section: Innovative Strategies For the Preparation Of Micro/nanofluid...mentioning

confidence: 99%

Merits and advances of microfluidics in the pharmaceutical field: design technologies and future prospects

et al. 2022

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Microfluidics is used to manipulate fluid flow in micro-channels to fabricate drug delivery vesicles in a uniform tunable size. Thanks to their designs, microfluidic technology provides an alternative and versatile platform over traditional formulation methods of nanoparticles. Understanding the factors that affect the formulation of nanoparticles can guide the proper selection of microfluidic design and the operating parameters aiming at producing nanoparticles with reproducible properties. This review introduces the microfluidic systems’ continuous flow (single-phase) and segmented flow (multiphase) and their different mixing parameters and mechanisms. Furthermore, microfluidic approaches for efficient production of nanoparticles as surface modification, anti-fouling, and post-microfluidic treatment are summarized. The review sheds light on the used microfluidic systems and operation parameters applied to prepare and fine-tune nanoparticles like lipid, poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles as well as cross-linked nanoparticles. The approaches for scale-up production using microfluidics for clinical or industrial use are also highlighted. Furthermore, the use of microfluidics in preparing novel micro/nanofluidic drug delivery systems is presented. In conclusion, the characteristic vital features of microfluidics offer the ability to develop precise and efficient drug delivery nanoparticles.

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Dual-Drug Delivery via Zein In Situ Forming Implants Augmented with Titanium-Doped Bioactive Glass for Bone Regeneration: Preparation, In Vitro Characterization, and In Vivo Evaluation

Cited by 16 publications

References 101 publications

Development and Evaluation of Chitosan Nanoparticles for Ocular Delivery of Tedizolid Phosphate

Development and Evaluation of Chitosan Nanoparticles for Ocular Delivery of Tedizolid Phosphate

Biomaterials for Tissue Engineering Applications and Current Updates in the Field: A Comprehensive Review

Merits and advances of microfluidics in the pharmaceutical field: design technologies and future prospects

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