Dual-Emissive Persistent Luminescence Nanoparticle-Based Charge-Reversible Intelligent Nanoprobe for Persistent Luminescence-Ratio Bioimaging along with Chemo-Photothermal Synergic Therapy

Abstract

Persistent luminescence nanoparticles (PLNPs) hold great promise for bioimaging owing to no demand for in situ excitation and negligible tissue autofluorescence interference. Nevertheless, huge challenges remain in the further development of single-emissive PLNPs due to the great variation of luminescence with time after excitation ceases. Herein, we report the controllable fabrication of dual-emissive monodispersed PLNPs (ZnGa2O4:Cr) by a surfactant-assisted hydrothermal method in combination with postcalcination for bioimaging. The prepared PLNPs emit luminescence at 508 and 714 nm with a constant luminescence ratio (I 508/I 714) for more than 1 h after UV excitation stops. Moreover, the prepared PLNPs give a constant I 508/I 714 ratio signal after repeated excitation by a LED lamp, allowing luminescence ratio imaging to ensure the long-term accuracy for in vivo imaging. In vivo ratio imaging demonstrates the potential of the prepared PLNPs for precision bioimaging. In addition, the prepared PLNPs have been applied to fabricate a theranostic nanoprobe with intelligent tumor-targeted imaging and chemo-photothermal synergistic therapy to further reveal their unique advantage for imaging guided therapy. We believe that the dual-emissive PLNPs will provide a promising nanoplatform for bioimaging and biomedical applications.

Ratiometric Luminescence Aptasensor Based on Dual-Emissive Persistent Luminescent Nanoparticles for Autofluorescence- and Exogenous Interference-Free Determination of Trace Aflatoxin B1 in Food Samples

“…The dual-emissive PLNP ZnGa 2 O 4 :Cr 0.0001 was prepared according to our previous work with a hydrothermal approach. 36 A total of 30.72 mg of CTAB was dispersed in ultrapure water. A volume of 24 mL of Ga(NO 3 ) 3 (0.4 M), 1.428 g of Zn(NO 3 ) 2 •6H 2 O, and 48 μL of Cr(NO 3 ) 3 (0.01 M) were added to the above solution in turn with vigorous stirring.…”

“…The dual-emissive PLNP ZnGa 2 O 4 :Cr 0.0001 was prepared according to our previous work with a hydrothermal approach . A total of 30.72 mg of CTAB was dispersed in ultrapure water.…”

Bacterial microenvironment-responsive dual-channel smart imaging-guided on-demand self-regulated photodynamic/chemodynamic synergistic sterilization and wound healing

“…Persistent luminescence nanoparticles (PLNPs) are a new generation of magical optical materials used for autofluorescence-free imaging due to their inherent merits of continuous in situ excitation-free and reactivated persistent luminescence. [29][30][31][32][33][34][35][36][37] However, false positive signal interference caused by the nontargeted aggregation of PLNPs still exists. Thus, developing bacteria-specific responsive PLNPs is significant in further improving their signal-to-noise ratio and sensitivity.…”

Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

“…To eliminate interference by autofluorescence, various detection strategies and materials have been developed for the construction of advanced fluorescence sensors. − Since their continuous luminescence lasts for several minutes to several days after the cessation of excitation, persistent luminescence nanoparticles (PLNPs) can realize autofluorescence-free detection without in situ excitation. These particles have many other advantages, such as low toxicity and a long-lasting stable luminescence intensity, and they are relatively simple to modify, making PLNPs attractive for biosensing − and bioimaging. , Despite these advantages, molecularly imprinted luminescence sensors based on PLNPs have not yet been reported.…”