Dual-functionalized magnetic bimetallic metal-organic framework composite for highly specific enrichments of phosphopeptides and glycopeptides

Pan, Yini; Zhang, Chenhui; Xiao, Ruili; Zhang, Lingyi; Zhang, Weibing

doi:10.1016/j.aca.2021.338412

Cited by 41 publications

(14 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The detection limits acquired in this work are lower than or equal to those in recently reported literature (Table S2, ESI †). [47][48][49][50] High selectivity is the key to ensure that an adsorbent can specifically capture phosphopeptides/glycopeptides from complicated samples. 51 To investigate the selectivity of phosphopeptide enrichment, b-casein digests mixed with 500-fold or 5000-fold bovine serum albumin (BSA) digests were selected as testing samples.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Tailoring a multifunctional magnetic cationic metal–organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides

et al. 2022

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…The detection limits acquired in this work are lower than or equal to those in recently reported literature (Table S2, ESI†). 47–50…”

Section: Resultsmentioning

confidence: 99%

Tailoring a multifunctional magnetic cationic metal–organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides

et al. 2022

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…Obviously, the commercial magnetic TiO 2 beads tended to monophosphopeptides, while the NiFe 2 O 4 @C@TiO 2 microspheres with multiple metal oxides had higher coverage for global phosphopeptide enrichment. In fact, the NiFe 2 O 4 @C@TiO 2 microspheres revealed better enrichment performance compared with most reported affinity materials. − The above results proved that unique hollow and porous structure, multiple metal-oxide affinity sites, and high specific surface area resulted in excellent performance for phosphopeptide enrichment in real biological samples (Figure ).…”

Section: Results and Discussionmentioning

confidence: 77%

Metal–Organic Framework-Derived Hollow and Hierarchical Porous Multivariate Metal-Oxide Microspheres for Efficient Phosphoproteomics Analysis

Luo

Yan

Zhang

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Pre-enrichment of the biological samples is a crucial step in phosphoproteomics research. At present, metal-oxide affinity chromatography (MOAC) is one of the most recognized enrichment strategy. Therefore, the design and preparation of a MOAC-based affinity material with better enrichment properties will be of great significance for the phosphoproteomics study. In this work, we obtained a novel multivariate metal-oxide microsphere (NiFe 2 O 4 @C@TiO 2 ) with a hollow and hierarchical porous structure through pyrolysis of TiO 2-modified Fe/Nibased metal−organic frameworks (MOFs). After pyrolysis, the carbon matrix derived from the MOFs provided support and porous properties. Meanwhile, multivariate metal oxides endowed the microspheres with an excellent magnetic response property and superior enrichment performance for phosphorylated biomolecules. The unique hollow and hierarchical porous structure greatly enhanced the diffusion of phosphorylated biomolecules. Therefore, the microspheres exhibited excellent enrichment performance for phosphorylated biomolecules: a large adsorption capacity (124 μmol g −1 ), excellent selectivity (αcasein/BSA, 1:5000, m/m), perfect size-exclusion performance (α-casein digests/α-casein/BSA, 1:500:500), and extremely low detection limit (2 fmol). Furthermore, the microspheres showed excellent enrichment performance in a series of real biological samples, such as nonfat milk, serum, saliva, rat brain tissue, and plasma exosomes of patients with esophageal cancer, which further demonstrated its huge application potential in MS-based phosphoproteomics research.

show abstract

“…A similar layer-by-layer assembly method was also adopted by Zhang and co-workers to fabricate Fe 3 O 4 @SiO 2 @(Zr-Ti-MOF) 10 -NH 2 , in which Fe 3 O 4 was coated with SiO 2 and amino groups, followed by alternating growth of Zr-MOF-NH 2 and Ti-MOF-NH 2 ( Pan et al, 2021 ). In a previous report, Zhang and co-workers discovered that the coexistence of Zr and Ti sharply promoted the efficiency of phosphopeptide enrichment.…”

Section: Different Types Of Hydrophilic Mofsmentioning

confidence: 99%

Advances in hydrophilic metal–organic frameworks for N-linked glycopeptide enrichment

Wei

Wang

et al. 2022

Front. Chem.

View full text Add to dashboard Cite

The comprehensive profiling of glycoproteins is of great significance for the timely clinical diagnosis and therapy. However, inherent obstacles hamper their direct analysis from biological samples, and specific enrichment prior to analysis is indispensable. Among the various approaches for glycopeptide enrichment, hydrophilic interaction liquid chromatography (HILIC) has attracted special focus, especially for the development of novel hydrophilic materials, which is the key of HILIC. Metal–organic frameworks (MOFs) are a type of porous materials constructed from the self-assembly of metal and organic linkers. Advantages such as high surface area, flexible pore size, and easy modification render hydrophilic MOFs as ideal candidates for HILIC, which has inspired many studies over the past years. In this review, advances in hydrophilic MOFs for N-linked glycopeptide enrichment are summarized. According to the synthesis strategies, those materials are categorized into three classes, namely pristine MOFs, MOFs with chemical modifications, and MOFs-derived composite. In each categorization, the preparation and the function of different moieties are covered, as well as the enrichment performances of sensitivity, selectivity, and practical application. Finally, a summary and future perspective on the applications of hydrophilic MOFs for N-linked glycopeptide enrichment are briefly discussed. This review is expected to raise awareness of the properties of hydrophilic MOFs and offer some valuable information to further research in glycoproteomics.

show abstract

Dual-functionalized magnetic bimetallic metal-organic framework composite for highly specific enrichments of phosphopeptides and glycopeptides

Cited by 41 publications

References 41 publications

Tailoring a multifunctional magnetic cationic metal–organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides

Tailoring a multifunctional magnetic cationic metal–organic framework composite for synchronous enrichment of phosphopeptides/glycopeptides

Metal–Organic Framework-Derived Hollow and Hierarchical Porous Multivariate Metal-Oxide Microspheres for Efficient Phosphoproteomics Analysis

Advances in hydrophilic metal–organic frameworks for N-linked glycopeptide enrichment

Contact Info

Product

Resources

About