Dual Gold Nanoparticle/Chemiluminescent Immunoassay for Sensitive Detection of Multiple Analytes

Abstract

Multiple antibiotics and mycotoxins usually simultaneously exist in foods, which poses a serious threat to human health. How to detect them in one test with high sensitivity and fidelity is challenging. In this study, we develop a dual readout lateral flow immunodetection platform that can quantitatively detect five kinds of antibiotics and five kinds of mycotoxins within one sample. The platform is composed of a chip and a portable readout instrument where gold nanoparticle (AuNP)-based and chemiluminescence immunoassays could be performed to reach a maximum throughput of 220 analytes in one setting. For a rapid screen, qualitative analysis by detecting the color change of the deposited AuNPs on the chip could be realized. For quantitative results, chemiluminescence imaging and analysis can be completed within 15 min. Apart from the high throughput and high efficiency, this platform has a high detection sensitivity. For instance, the limit of detection (LOD) for thiamphenicol (a representative antibiotic) and fumonisins B1 (a representative mycotoxin) is 8 times and 40 times lower than those of the previously reported methods, respectively. Thus, this dual readout immunodetection platform is promising as a universal device for rapid and quantitative detection of multiple analytes with high throughput, high sensitivity, and high fidelity.

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

“…Cellulose paper-based microfluidics can handle 0.1–100 μL of liquid through millimeter-scale fluidic channels [ 119 ]. As one of the popular applications of paper-based materials, lateral flow assay (LFA) is a user-friendly diagnostic tool [ [120] , [121] , [122] , [123] , [124] ].

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Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

“…In food safety testing, multiple foodborne pathogens may cause the same symptoms (such as diarrhea), and the targets are often unknown strains before detection, so the simultaneous detection of multiple bacteria in a single sample is becoming increasingly important. To further increase the detection throughput, the future multiplex LFSBs will be focused on designing multiple T-lines in a single test strip or using multiple test strips with a single T-line. , The realization of multiple detection is a major issue that needs to be addressed in current research and commercial LFSB development.…”

“…To further increase the detection throughput, the future multiplex LFSBs will be focused on designing multiple T-lines in a single test strip or using multiple test strips with a single T-line. 150,151 The realization of multiple detection is a major issue that needs to be addressed in current research and commercial LFSB development.…”

Anthracycline-Functionalized Dextran as a New Signal Multiplication Tagging Approach for Immunoassay

“…Hence, non-enzymatic labels for detection are being and have been developed, such as beads [ 19 ] and metal nanoparticles [ 16 , 17 , 20 , 21 , 22 , 23 , 24 , 25 ]. Ye et al used gold vesicles encapsulated with Pd-Ir nanoparticles (Pd-Ir NPs@GVs) as peroxidase mimics by raising the temperature.…”