Dual guide RNA-mediated concurrent C&amp;G-to-T&amp;A and A&amp;T-to-G&amp;C conversions using CRISPR base editors

Zhao, Yuting; Li, Min; Liu, Jie; Xue, Xiaowen; Zhong, Jingli; Lin, Jianxiang; Ye, Bo; Qiao, Yong

doi:10.1016/j.csbj.2022.12.055

Cited by 4 publications

(1 citation statement)

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“…Such editors have detectable off-target activity, similarly to CBEs and ABEs, and editing efficiency is often lower than efficiency of CBE or ABE. Still, Dual BE is more versatile than CBE or ABE [42,43].…”

Section: Structure Mechanism Of Action and Dna Lesions Caused By Cris...mentioning

confidence: 99%

CRISPR/Cas9 as a Mutagenic Factor

Shumega,

Pavlov,

Chirinskaite

et al. 2024

IJMS

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The discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites. DNA repair systems differing in accuracy participate in establishing desired genetic changes but also introduce unwanted mutations, that may lead to hereditary, oncological, and other diseases. New approaches to alleviate the risks associated with genome editing include attenuating the off-target activity of editing complex through the use of modified forms of Cas9 nuclease and single guide RNA (sgRNA), improving delivery methods for sgRNA/Cas9 complex, and directing DNA lesions caused by the sgRNA/Cas9 to non-mutagenic repair pathways. Here, we have described CRISPR/Cas9 as a new powerful mutagenic factor, discussed its mutagenic properties, and reviewed factors influencing the mutagenic activity of CRISPR/Cas9.

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Section: Structure Mechanism Of Action and Dna Lesions Caused By Cris...mentioning

confidence: 99%