Duck Tembusu virus infection causes testicular atrophy

Huang, Yufei; Chu, Xiaoya; Zhang, Yafei; Yang, Sheng; Shi, Yonghong; Wu, Jingxian; Chen, Qiusheng

doi:10.1016/j.theriogenology.2022.05.012

Cited by 3 publications

(1 citation statement)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tembusu virus (TMUV), a positive-sense single-stranded RNA virus belonging to the Flaviviridae family and Flavivirus genus ( 1 ), was first detected in the Malaysian Culex mosquito in 1955 and became endemic in duck farms in southeastern coastal China in 2010 ( 2 , 3 ). DTMUV has a wide range of hosts, such as chickens, ducks, geese, pigeons, and sparrows, and the diseased ducks show a series of symptoms, including ovarian hemorrhage and necrosis, decreased egg production, ataxia, diarrhea, and enlarged spleen and liver, with an incidence of up to 100% and mortality rates between 5 and 30% ( 4 , 5 ); moreover, younger ducks exhibit more serious pathogenicity ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

et al. 2023

View full text Add to dashboard Cite

Duck Tembusu virus (DTMUV), duck circovirus (DuCV), and new duck reovirus (NDRV) have seriously hindered the development of the poultry industry in China. To detect the three pathogens simultaneously, a multiplex digital PCR (dPCR) was developed and compared with multiplex qPCR in this study. The multiplex dPCR was able to specifically detect DTMUV, DuCV, and NDRV but not amplify Muscovy duck reovirus (MDRV), Muscovy duck parvovirus (MDPV), goose parvovirus (GPV), H4 avian influenza virus (H4 AIV), H6 avian influenza virus (H6 AIV), and Newcastle disease virus (NDV). The standard curves showed excellent linearity in multiplex dPCR and qPCR and were positively correlated. The sensitivity results showed that the lowest detection limit of multiplex dPCR was 1.3 copies/μL, which was 10 times higher than that of multiplex qPCR. The reproducibility results showed that the intra- and interassay coefficients of variation were 0.06–1.94%. A total of 173 clinical samples were tested to assess the usefulness of the method; the positive detection rates for DTMUV, DuCV, and NDRV were 18.5, 29.5, and 14.5%, respectively, which were approximately 4% higher than those of multiplex qPCR, and the kappa values for the clinical detection results of multiplex dPCR and qPCR were 0.85, 0.89, and 0.86, indicating that the two methods were in excellent agreement.

show abstract

Section: Introductionmentioning

confidence: 99%

Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

et al. 2023

View full text Add to dashboard Cite

show abstract

Isolation, identification, and pathogenicity evaluation of a novel Cluster 3 Tembusu virus isolated from geese in China

Wang,

Chen,

Ding

et al. 2025

Poultry Science

View full text Add to dashboard Cite

One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses

Wang,

Liu,

Cheng

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Duck Tembusu virus (DTMUV), duck hepatitis virus (DHV), Muscovy duck reovirus (MDRV), and Muscovy duck parvovirus (MDPV) represent four emergent infectious diseases impacting waterfowl, which can be challenging to differentiate due to overlapping clinical signs. In response to this, we have developed a one-step multiplex real-time fluorescence quantitative reverse transcription PCR (qRT-PCR) assay, capable of simultaneously detecting DTMUV, DHV, MDRV, and MDPV. This method exhibits high specificity, avoiding cross-reactivity with other viruses such as Fowl adenoviruses (FADV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), Haemophilus paragallinarum (Hpg), duck circovirus (DUCV), goose astrovirus (GoAstV), and mycoplasma gallisepticum (MG). The limit of detection (LOD) established for DTMUV, DHV, MDRV, and MDPV was determined to be 27 copies/μL. In the repeatability test, the intra-assay and inter-assay coefficients of variation (CVs) of the recombinant plasmid standard were less than 2%. Utilizing this method, we analyzed 326 clinical specimens sourced from Guangxi over the period spanning October 2021 through December 2023, yielding promising and precise outcomes. The qRT-PCR method established herein exhibits commendable specificity, sensitivity, and repeatability. Furthermore, it boasts a high clinical detection rate, making it a highly effective tool for diagnosing these pathogenic agents in waterfowl.

show abstract

Duck Tembusu virus infection causes testicular atrophy

Cited by 3 publications

References 28 publications

Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

Multiplex digital PCR: a superior technique to qPCR for the simultaneous detection of duck Tembusu virus, duck circovirus, and new duck reovirus

Isolation, identification, and pathogenicity evaluation of a novel Cluster 3 Tembusu virus isolated from geese in China

One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses

Contact Info

Product

Resources

About