Durable protein lattices of clathrin that can be functionalized with nanoparticles and active biomolecules

Dannhauser, Philip N.; Platen, Mitja; Böning, Heike; Schaap, Iwan A. T.

doi:10.1038/nnano.2015.206

Cited by 13 publications

(13 citation statements)

References 33 publications

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“…However, as the neural network ages (either with CCL11 treatment or at week 2), the efficiency of macro‐pinocytosis was more greatly reduced. This finding has implications for the efficacy of MNP designed to target specific endocytic pathways …”

Section: Resultsmentioning

confidence: 97%

The Age of Cortical Neural Networks Affects Their Interactions with Magnetic Nanoparticles

Tay

Kunze

Jun

et al. 2016

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Despite increasing use of nanotechnology in neuroscience, the characterization of interactions between magnetic nanoparticles (MNPs) and primary cortical neural networks remains underdeveloped. In particular, how the age of primary neural networks affects MNP uptake and endocytosis is critical when considering MNP-based therapies for age-related diseases. In this study, primary cortical neural networks are cultured up to 4 weeks and with CCL11/eotaxin, an age-inducing chemokine, to create aged neural networks. As the neural networks aged, their association with membrane-bound starch-coated ferromagnetic nanoparticles (fMNPs) increased while their endocytic mechanisms are impaired, resulting in reduced internalization of chitosan-coated fMNPs. The age of the neurons also negates the neuro-protective effects of chitosan coatings on fMNPs, attributing to decreased intracellular trafficking and increased co-localization of MNPs with lysosomes. These findings demonstrates the importance of age and developmental stage of primary neural cells when developing in vitro models for fMNP therapeutics targeting age-related diseases.

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Section: Resultsmentioning

confidence: 97%

The Age of Cortical Neural Networks Affects Their Interactions with Magnetic Nanoparticles

Tay

Kunze

Jun

et al. 2016

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“…As such, new materials with dynamic topological edge modes can be already engineered at micron and nanoscale using the recent advances in self or directed assembled materials. Indeed, methods to design and self-assemble nano-structures with programmable lattices are widely available and, for example, DNA and proteins, such as clathrin, have been used extensively to create 3D lattices or other solid state-like structures, 3D origami and even origami lattices 37 38 .…”

Section: Discussionmentioning

confidence: 99%

Dynamical Majorana edge modes in a broad class of topological mechanical systems

et al. 2017

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Mechanical systems can display topological characteristics similar to that of topological insulators. Here we report a large class of topological mechanical systems related to the BDI symmetry class. These are self-assembled chains of rigid bodies with an inversion centre and no reflection planes. The particle-hole symmetry characteristic to the BDI symmetry class stems from the distinct behaviour of the translational and rotational degrees of freedom under inversion. This and other generic properties led us to the remarkable conclusion that, by adjusting the gyration radius of the bodies, one can always simultaneously open a gap in the phonon spectrum, lock-in all the characteristic symmetries and generate a non-trivial topological invariant. The particle-hole symmetry occurs around a finite frequency, and hence we can witness a dynamical topological Majorana edge mode. Contrasting a floppy mode occurring at zero frequency, a dynamical edge mode can absorb and store mechanical energy, potentially opening new applications of topological mechanics.

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“…Clathrin has been applied to various biotech materials as substrates. The materials that have been successfully added to clathrin are graphene, polymer, glass, and metal [15]. Figure 2 shows the application of clathrin on various materials.…”

Section: Introductionmentioning

confidence: 99%

Performance Improvement of Dye-Sensitized Solar Cell- (DSSC-) Based Natural Dyes by Clathrin Protein

Trihutomo

Soeparman

Widhiyanuriyawan

et al. 2019

International Journal of Photoenergy

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Dye-Sensitized Solar Cell (DSSC) is a solar cell device that works using electrochemical principles in which sensitive dyes are absorbed in the TiO2 photoelectrode layer. The problem of DSSC-based natural dyes is the lower efficiency than silicon solar cells. This low efficiency is due to the barrier of electron transfer in the TiO2 semiconductor layer. In this study, the addition of clathrin protein to the TiO2 layer was used to increase electron transfer in the semiconductor layer resulting in improved DSSC performance. Clathrin is a protein that plays a role in the formation of transport vesicle membrane in eukaryotic cells. The method used in this study is clathrin protein with a concentration of 0%, 25%, 50%, and 75% added to TiO2 in DSSC structure. Photovoltaic characteristics of DSSC were measured using a data logger to determine the performance of DSSC, layer morphology was analyzed using Scanning Electron Microscopy (SEM), the element content in DSSC was analyzed using Energy-Dispersive X-ray Spectroscopy (EDS), and functional groups in DSSC layers were analyzed using Fourier-Transform Infrared Spectroscopy (FTIR). The result of this study is the addition of clathrin protein can improve DSSC performance, which resulted in the highest performance of DSSC on 75% clathrin protein addition with efficiency=1.465%, Isc=5.247 mA, and Voc=657 mV. From the results of SEM analysis, it appears that clathrin protein molecules fill the cavities in TiO2 molecules. EDS analysis shows an increase in carbon, oxygen, and phosphorus content in TiO2 layers with increasing clathrin protein concentration. FTIR analysis shows an increasingly sharp absorption in the FTIR spectrum of protein-forming functional groups by increasing clathrin protein concentration in DSSC.

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Durable protein lattices of clathrin that can be functionalized with nanoparticles and active biomolecules

Cited by 13 publications

References 33 publications

The Age of Cortical Neural Networks Affects Their Interactions with Magnetic Nanoparticles

The Age of Cortical Neural Networks Affects Their Interactions with Magnetic Nanoparticles

Dynamical Majorana edge modes in a broad class of topological mechanical systems

Performance Improvement of Dye-Sensitized Solar Cell- (DSSC-) Based Natural Dyes by Clathrin Protein

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