Dye penetration in dry and water‐filled gaps along root fillings

Kontakiotis, Evangelos G.; Georgopoulou, Maria; Morfis, A

doi:10.1046/j.1365-2591.2001.00362.x

Cited by 19 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A large number of studies have used methylene blue as a dye (28,34,45) because it is inexpensive, easy to handle, has a high degree of staining (46) and has a molecular weight lower than that of bacterial toxins (47). Some researchers have suggested that methylene blue exhibits leakage similar to butyric acid (48), a microbial metabolic product with greater penetration than Indian ink.…”

Section: Resultsmentioning

confidence: 99%

“…However, it is difficult to eliminate the trapped air by applying low pressure to small empty spaces, including those measuring 2 μm in diameter, which are permeable to bacteria (53). Kontakiotis et al (45) investigated the influence of hydration on voids within the root canal obturation materials using a fluid transport model and dye penetration, in which air was applied to eliminate water from the voids in one group and showed that methylene blue penetrated more easily into dry gaps than into water-filled gaps. Methylene blue penetrated along air-filled gaps by capillary action, whereas it penetrated into water-filled gaps by diffusion (21).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Importance and methodologies of endodontic microleakage studies: A systematic review

Jafari

2017

J Clin Exp Dent

View full text Add to dashboard Cite

IntroductionIt is very important to obtain a tight seal in obturated root canal, making it necessary to conduct clinical or laboratory studies on the sealability of endodontic materials. Different methodologies have been historically used to assess microleakage of different endodontic materials. The aim of the present study was to comprehensively review different material testing methods used in microleakage studies, their interpretation and importance in endodontic literature.Material and MethodsA systematic search was conducted in Medline, Cochrane, and Web of Science databases. In addition, the reference lists of review articles on the topic were searched. No language restriction was applied. Two independent reviewers screened the article.ResultsMicroleakage is considered the single most important risk factor responsible for apical periodontitis. Dye penetration, dye diffusion, bacterial and endototoxin infiltration, fluid filtration, glucose, caffeine and protein infiltration, radioisotope penetration, animal studies, and electrochemical or 3D evaluation are different methodologies used to assess dental leakage. 91 out of 177 articles in the primary search were included in the study. These methods are very divergent in their viewpoints; that is why their results cannot be easily compared. It is necessary to standardize microleakage detection methods in order to more correctly evaluate the phenomena that are found between the root canal wall and the root canal filling materials.ConclusionsAll the methods are useful if studies are performed strictly with large sample sizes and proper control groups and if the technique can be standardized. Furthermore, more evaluations of the reliability of the methods are strongly recommended. Key words:Dental leakage, review, root canal, material testing methods, data interpretation.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Importance and methodologies of endodontic microleakage studies: A systematic review

Jafari

2017

J Clin Exp Dent

View full text Add to dashboard Cite

show abstract

“…A large number of studies used methylene blue as dye (17,19,29) because it is inexpensive, easy to manipulate, has a high degree of staining and a molecular weight even lower than that of bacterial toxins (17). It has been suggested that methylene blue presents the same leakage as butyric acid (30), a microbial metabolic product that has greater penetration than Indian ink.…”

Section: Methodology That Uses Dyesmentioning

confidence: 99%

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

Veríssimo

Vale

2006

Journal of Oral Science

137

110

View full text Add to dashboard Cite

Keywords: dye dilution technique; dyes; filtration. IntroductionMicroorganisms present inside root canals may remain active in the dentinal tubules even after vigorous chemicalmechanical preparation. Thus, perfect apical sealing is desirable to prevent the remaining bacteria and their endotoxins from reaching the root apex (1,2). Apical leakage is considered to be a common cause for endodontic therapy failure, and is influenced by many variables such as different filling techniques, the physical and chemical properties of sealers and the presence or absence of a smear layer (1,3). In coronal leakage, the canal may be recontaminated in various ways such as contact between the oral bacterial flora and root canal tubule inlets. However, it most frequently occurs as a result of loss of temporary filling, or inadequate endodontic filling or crown sealing (4,5).According to Timpawat et al. (6), endodontic sealers are used to eliminate the interface between the gutta-percha and the dentinal walls. Leakage may, however, occur at the interfaces between the sealer and dentin, sealer and gutta-percha and in spaces within the sealer itself. Thus, the quality of the filling depends largely on the sealing capacity offered by sealers (7,8).Methodologies in vitro are used to estimate sealing quality, generally by measuring microleakage that allows the tracer agent to penetrate the filled canal. Commonly used tracers are dyes, radioisotopes, bacteria and their products, such as endotoxins. Bacteria used as tracers most closely approximate what happens clinically in terms of leakage (9-11). Other methodologies, such as fluid filtration and dye extraction methods have also been used, their main advantage being high reproducibility (1,(12)(13)(14).The aim of this review was to critically analyze the various methodologies reported in the literature for assessing root canal sealing capacity. Apparently, they are not standardized, which makes it difficult to compare the results obtained when different methodologies are used, even though the same substrate is being assessed. Literature ReviewAlthough there are many leakage studies, there is no consensus about the endodontic sealer and core material sealing capacities. One of the reasons is that investigations do not use a standardized methodology and this frequently

show abstract

“…In this study, methylene blue dye penetration was used. Dye penetration experiment using various types of dyes (eosin, methylene blue, black India ink, Procion brilliant blue, and others) is widely used 32,33) because it is inexpensive, easy to manipulate, and it has a high degree of staining property. Also it has molecular weight even lower than that of bacterial toxins, so it has similar leakage to butyric acid, which is a microbial metabolic product 34) .…”

Section: Discussionmentioning

confidence: 99%

<i>In vitro</i> microleakage of six different dental materials as intraorifice barriers in endodontically treated teeth

et al. 2015

View full text Add to dashboard Cite

The aim of this study was to compare the coronal sealing ability of six different dental materials: Three MTA-based cements and three established restorative materials by in vitro dye penetration method. For in vitro infiltration experiments, seventy extracted single-rooted human teeth were used. After crowns of teeth were reduced, root canals were prepared, and filled with gutta-percha cone. Teeth were randomly divided into 6 groups with 10 teeth per group. The orifice of each tooth was prepared to 3 mm depth and filled with the following materials: (I) ProRoot WMTA; (II) EndoCem Zr; (III) Angelus White; (IV) LuxaCore; (V) Fuji II LC; and (VI) Elite. After 5,000 cycles of thermocycling between 5°C and 55°C, dye penetration of each specimen was measured. The order of less dye infiltration of coronal filling materials was: ProRoot WMTA

show abstract

Dye penetration in dry and water‐filled gaps along root fillings

Abstract: Methylene blue penetrates along root fillings more easily in dry gaps than in water-filled gaps.

Cited by 19 publications

References 9 publications

Importance and methodologies of endodontic microleakage studies: A systematic review

Importance and methodologies of endodontic microleakage studies: A systematic review

Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review

<i>In vitro</i> microleakage of six different dental materials as intraorifice barriers in endodontically treated teeth

Contact Info

Product

Resources

About