Dye screening and signal-to-noise ratio for retrogradely transported voltage-sensitive dyes

Tsau, Yang; Wenner, Peter; O’Donovan, Michael J.; Cohen, Lawrence B.; Loew, Leslie M.; Wuskell, Joseph P.

doi:10.1016/s0165-0270(96)00109-4

Cited by 54 publications

(41 citation statements)

References 15 publications

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“…All dyes prepared for this study were synthesized using variations of general procedures already described (Hassner et al, 1984;Tsau et al, 1996). The final dye products were purified by chromatographic techniques (column or chromatatron chromatography) and obtained as viscous hygroscopic oils that were best handled in ethanol solution.…”

Section: Dye Synthesismentioning

confidence: 99%

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Obaid

Loew

Wuskell

et al. 2004

Journal of Neuroscience Methods

121

108

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Section: Dye Synthesismentioning

confidence: 99%

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Obaid

Loew

Wuskell

et al. 2004

Journal of Neuroscience Methods

121

108

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“…Another favorable feature of these dyes is their high fluorescence quantum efficiency when bound to membranes but negligible fluorescence in aqueous solution; thus, only stained cells contribute to the fluorescence signal even if the experimental protocol does not permit washing away the staining solution. The existing repertoire of styryl potentiometric dyes have varying solubilities, lipid avidities, tissue penetrability, and ionic charges that allows them to be customized for specific types of experimental demands (Antic et al, 1992Obaid et al, 2004;Tsau et al, 1996;Zecevic, 1996). We have also developed dyes for covalent labeling so as to map electric profiles at specific sites in proteins (Asamoah et al, 2003).…”

mentioning

confidence: 99%

Synthesis, spectra, delivery and potentiometric responses of new styryl dyes with extended spectral ranges

Wuskell

Boudreau

Wei

et al. 2006

Journal of Neuroscience Methods

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“…On the other hand, the maximum fractional change has not increased in recent years, and most improvements (e.g. Grinvald et al 1982;Antic & Zecevic 1995;Momose-Sato et al 1995;Tsau et al 1996) have involved synthesizing analogues that work well on new preparations.…”

Section: Future Directions (A) Organic Voltage-sensitive Dyesmentioning

confidence: 99%

Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes

Homma

Baker

Liu

et al. 2009

Phil. Trans. R. Soc. B

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This review presents three examples of using voltage-or calcium-sensitive dyes to image the activity of the brain. Our aim is to discuss the advantages and disadvantages of each method with particular reference to its application to the study of the brainstem. Two of the examples use wide-field (onephoton) imaging; the third uses two-photon scanning microscopy. Because the measurements have limited signal-to-noise ratio, the paper also discusses the methodological aspects that are critical for optimizing the signal.The three examples are the following. (i) An intracellularly injected voltage-sensitive dye was used to monitor membrane potential in the dendrites of neurons in in vitro preparations. These experiments were directed at understanding how individual neurons convert complex synaptic inputs into the output spike train. (ii) An extracellular, bath application of a voltage-sensitive dye was used to monitor population signals from different parts of the dorsal brainstem. We describe recordings made during respiratory activity. The population signals indicated four different regions with distinct activity correlated with inspiration. (iii) Calcium-sensitive dyes can be used to label many individual cells in the mammalian brain. This approach, combined with two-photon microscopy, made it possible to follow the spike activity in an in vitro brainstem preparation during fictive respiratory rhythms.The organic voltage-and ion-sensitive dyes used today indiscriminatively stain all of the cell types in the preparation. A major effort is underway to develop fluorescent protein sensors of activity for selectively staining individual cell types.

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Dye screening and signal-to-noise ratio for retrogradely transported voltage-sensitive dyes

Cited by 54 publications

References 15 publications

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Novel naphthylstyryl-pyridinium potentiometric dyes offer advantages for neural network analysis

Synthesis, spectra, delivery and potentiometric responses of new styryl dyes with extended spectral ranges

Wide-field and two-photon imaging of brain activity with voltage- and calcium-sensitive dyes

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