In the engineering of noise and vibration, one of the most important things is to determine the dynamic forces caused by engine vibration at the excitation locations on the engine mount. With the determined dynamic forces, the structure dynamic behavior and aircraft cabin noise caused by engine vibration can be predicted and corresponding methods can be used to resolve possible problems. However, it is impossible to measure the dynamic forces at the engine mount caused by engine vibration directly when aircraft is in flight condition. In this paper, an indirect method called the least-squares scheme is implemented to back-calculate the engine mount dynamic forces with the measured frequency response function (FRF) matrices on the ground and measured structural acceleration in the flight condition. The norm condition number is applied to evaluate the inversion process and it shows that these identified dynamic forces on the engine mount meet the requirement of engineering.