Dynamic behaviour of selected PET tracers in embryonated chicken eggs

Gebhardt, Peter; Würbach, Lydia; Heidrich, Alexander; Heinrich, Liesa; Walther, M.; Opfermann, Thomas; Sorensen, B.; Saluz, Hans Peter

doi:10.1016/j.remn.2013.07.002

Cited by 15 publications

(15 citation statements)

References 19 publications

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“…Similar approaches have been reported for chicken embryos successfully applying isoflurane. 14,29 Different studies investigated the use of cooling, 30 muscle relaxing agents 31–34 and ketamine; 32 however, these concepts usually requiring invasive preparation, that is, partially removing the egg shell to get access to the chorion-allanois-membrane and/or vitelline vessels for intravasal agent application. Also, the aforementioned studies consider chicken embryos.…”

Section: Discussionmentioning

confidence: 99%

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Freesmeyer

Hermeyer

Kühnel

et al. 2022

Exp Biol Med (Maywood)

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In-ovo imaging using ostrich eggs has been described as a potential alternative to common animal testing. The main advantage is its independence from small animal imaging devices as ostrich eggs provide good image quality on regular CT, MRI, or PET used in examinations of humans. However, embryonal motion during dynamic imaging studies produce artifacts. The aims of this study were (1) to explore the feasibility of biomagnetism to detect cardiac signals and embryonal motion and to use these findings (2) to investigate the effect of isoflurane anesthesia on ostrich embryos. A standard magnetoencephalography developed for brain studies was used to detect embryonal signals of ostrich eggs on developmental day 34. Signals were instantly shown on a screen and data were also postprocessed. For assessing the effects of anesthesia, nine ostrich eggs were investigated using isoflurane 6% for 90 min. Biomagnetic signals were recorded simultaneously. A control group consisting of eight different ostrich eggs was also investigated. Cardiac signals similar to electrocardiography were observed in all eggs. Postprocessing revealed frequent motion of embryos without anesthesia. The exposure to isoflurane led to a significant decrease in motion signals in 9/9 ostrich embryos after 8 min. Motion was significantly reduced in the isoflurane group versus control group. There were no isoflurane-related deaths. This study shows that biomagnetism is feasible to detect cardiac signals and motion of ostrich embryos in-ovo. Application of isoflurane is safe and leads to a rapid decrease in embryonal motion, which is an important prerequisite for the implementation of in-ovo imaging using ostrich eggs.

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Section: Discussionmentioning

confidence: 99%

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Freesmeyer

Hermeyer

Kühnel

et al. 2022

Exp Biol Med (Maywood)

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“…Although tumors have been already successfully established on the CAM [4,[20][21][22][23][24][25][26][27][28][29], and PET-imaging studies are available [10,24,25,[30][31][32][33][34][35], systematic analyses of their use to evaluate the biodistribution of radiopharmaceuticals and direct comparisons with the in vivo gold standard are still rare [10].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

Löffler

Hamp

Scheidhauer

et al. 2021

Cancers

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Assessment of biodistribution and specific tumor accumulation is essential for the development of new radiopharmaceuticals and requires animal experiments. The HET-CAM (hens-egg test—chorioallantoic membrane) model can be used in combination with the non-invasive imaging modalities PET and MRI for pre-selection during radiopharmaceutical development to reduce the number of animal experiments required. Critical to the acceptance of this model is the demonstration of the quantifiability and reproducibility of these data compared to the standard animal model. Tumor accumulation and biodistribution of the PSMA-specific radiotracer [18F]F-siPSMA-14 was analyzed in the chick embryo and in an immunodeficient mouse model. Evaluation was based on MRI and PET data in both models. γ-counter measurements and histopathological analyses complemented these data. PSMA-specific accumulation of [18F]F-siPSMA-14 was successfully demonstrated in the HET-CAM model, similar to the results obtained by mouse model studies. The combination of MR and PET imaging allowed precise quantification of peptide accumulation, initial assessment of biodistribution, and accurate determination of tumor volume. Thus, the use of the HET-CAM model is suitable for the pre-selection of new radiopharmaceuticals and potentially reduces animal testing in line with the 3Rs principles of animal welfare.

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“…Inoculation and growth of organoids and tissues have successfully been performed contributing to the translational value of this model [85][86][87]. While this model has been used for longer than a century in tumor research and toxicity testing [88], it only gained interest in preclinical imaging in the last decade [89,90]. The distribution of different radiopharmaceuticals in the chicken embryonal organism has been studied and compared to mice, providing the first evidence for the suitability of this model in tracer development [91,92].…”

Section: In Vivo Modelsmentioning

confidence: 99%

Experimental Nuclear Medicine Meets Tumor Biology

et al. 2022

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Personalized treatment of cancer patients demands specific and validated biomarkers for tumor diagnosis and therapy. The development and validation of such require translational preclinical models that recapitulate human diseases as accurately as possible. Moreover, there is a need for convergence of different (pre)clinical disciplines that openly share their knowledge and methodologies. This review sheds light on the differential perception of biomarkers and gives an overview of currently used models in tracer development and approaches for biomarker discovery.

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Dynamic behaviour of selected PET tracers in embryonated chicken eggs

Cited by 15 publications

References 19 publications

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

In-ovo imaging using ostrich eggs: Biomagnetism for detection of cardiac signals and embryonal motion

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

Experimental Nuclear Medicine Meets Tumor Biology

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