Dynamic capillary coatings for electroosmotic flow control in capillary electrophoresis

Melanson, Jeremy E.; Baryla, Nicole E.; Lucy, Charles A.

doi:10.1016/s0165-9936(01)00067-x

Cited by 131 publications

(93 citation statements)

References 36 publications

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“…The effect of applied voltage was also investigated as it is well known that increasing the voltage gives rise to shorter migration times (Melanson et al, 2001). However the generation of Joule heat may limit the theoretical gain in the resolution and efficiency when the voltage is increased.…”

Section: Effect Of Applied Voltagementioning

confidence: 99%

Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis

Elbashir

Saad

Ali

et al. 2008

Biomedical Chromatography

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A capillary electrophoretic (CE) method for the baseline separation of the enantiomers of primaquine diphosphate (PQ) and quinocide (QC) (a major contaminant) in pharmaceutical formulations is proposed. Both components were separated under the following conditions: 50 mm tris phosphate buffer (pH 3.0) containing 15 mm hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD) as background electrolyte; applied voltage, 16 kV; capillary temperature, 25 degrees C; detection wavelength, 254 nm; hydrostatic injection, 10 s. The separations were conducted using a 35 cm length and 50 microm i.d. uncoated fused silica capillary column. Under the optimized conditions, the components were successfully separated in about 5 min. Intraday precision of migration time and corrected peak areas when expressed as relative standard deviation ranged from 0.17 to 0.45 and 2.60 to 3.94%, respectively, while the interday precision ranged from 2.59 to 4.20 and 3.15 to 4.21%, respectively. After the validation exercise, the proposed method was applied for the determination of QC impurity in PQ formulations.

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Section: Effect Of Applied Voltagementioning

confidence: 99%

Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis

Elbashir

Saad

Ali

et al. 2008

Biomedical Chromatography

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“…The unpredictable EOF and strong wall adsorption of analyte may ruin the precision and limit the CE application in quantitative analysis. A number of methods have been developed for EOF control in a capillary, such as external electric field [14], physically adsorbed or covalent static coating [15], and dynamic coating [16]. Among them, the surfactant-based dynamic coating is a favorable method due to its versatility, stability, and facility.…”

Section: Introductionmentioning

confidence: 99%

Cationic gemini surfactant as dynamic coating in CE for the control of EOF and wall adsorption

Liu¹,

Li²,

Tang³

et al. 2007

Electrophoresis

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The cationic gemini surfactant ethylene bis(1-dodecyldimethylammonium) dibromide was used as a dynamic coating to control EOF and prevent wall adsorption of basic proteins in CE for the first time. This gemini surfactant shows a more powerful capability in EOF reversal than traditional single-chained surfactant. The gemini surfactant reverses the EOF at a concentration level even less than 0.01 mM, and the EOF magnitude is affected by surfactant concentration, pH, ionic strength, and ions added in buffer. Highly efficient and rapid protein separation (N > 300,000) was obtained with buffer containing 2 mM gemini surfactant under pH ranging from 3 to 6. The effects of surfactant and buffer concentration on protein separation were investigated in detail. Under the optimal conditions, good repeatability (RSD of migration time <0.6% for run-to-run and <2.5% for day-to-day assays) and recovery (>90%) of tested proteins were obtained. This new dynamic coating is also suitable for biosample analysis.

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“…Dynamic capillary coating procedures are easy to perform under standard laboratory conditions and provide an effective way to alter EOF or diminish protein adsorption, since these coating materials are simply added to the BGE as depicted in Scheme 1a [7,10]. Due to their equilibrium based nature, these coatings need to be continuously regenerated by refreshing the adsorbed layer on the capillary wall [1].…”

Section: Dynamic Coating Approachesmentioning

confidence: 99%

Recent advances in column coatings for capillary electrophoresis of proteins

Hajba

Guttman

2017

TrAC Trends in Analytical Chemistry

121

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Capillary coatings effectively improve the separation performance of proteins in capillary electrophoresis, mainly by reducing protein adsorption onto the inner capillary wall and by regulating the electroosmotic flow (EOF) to accommodate the separation problem in hand. In the first part of this review the newest trends in dynamic and permanent capillary coatings are summarized and discussed in detail. In the second part the application of nanomaterials as novel capillary coating materials is conversed. Nanomaterials have great potential in capillary coating preparations based on their advantageous properties such as large surface-to-volume ratios and a wide variety of chemistry options. Finally, some future prospective of capillary coatings in the emerging field of proteomics are given.

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Dynamic capillary coatings for electroosmotic flow control in capillary electrophoresis

Cited by 131 publications

References 36 publications

Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis

Enantioselective analysis of primaquine and its impurity quinocide by capillary electrophoresis

Cationic gemini surfactant as dynamic coating in CE for the control of EOF and wall adsorption

Recent advances in column coatings for capillary electrophoresis of proteins

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