Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model

Zheng, Yi; Qin, Jining; Wang, Xin; Peng, Zhiyou; Hou, Peiyong; Lu, Xinwu

doi:10.2147/ijn.s118328

Cited by 9 publications

(8 citation statements)

References 29 publications

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“…Recently, issues have driven much interest in allogeneic stem cells for regeneration therapy, in which cells obtained from health donors can be quality‐controlled and readily available as an “off‐the‐shelf” product for urgent application in clinical 8,9 . Previous studies by us 10‐12 and others 13,14 have confirmed the significance of allogeneic ASCs in ischemic muscle repair, whereas the specific mechanism has seldom been explored. Recently, researchers have demonstrated that the function of mesenchymal stem cells (MSCs) in promoting tissue repair is mainly related to their paracrine effect rather than their direct implantation and differentiation 15,16 .…”

Section: Introductionmentioning

confidence: 99%

Allogeneic adipose-derived stem cells promote ischemic muscle repair by inducing M2 macrophage polarization via the HIF-1α/IL-10 pathway

et al. 2020

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Adipose-derived mesenchymal stem cells (ASCs) are multipotent stromal cells that possess considerable therapeutic potential for tissue remodeling. However, their protective mechanism in critical limb ischemia has not been fully defined. After the occlusion of blood vessels, hypoxia becomes a prominent feature of the ischemic limb. This study investigated the immunomodulatory effect of ASCs on ischemic muscle repair and explored the specific mechanism. We found that the ability of RAW264.7 cells to migrate was impaired in hypoxia, whereas coculturing with ASCs could enhance the migration capacity. In addition, under hypoxic conditions, the paracrine effect of ASCs was enhanced and ASCs could induce RAW264.7 macrophages toward the antiinflammatory M2 phenotype. We further demonstrated that ASCs-derived interleukin 10 (IL-10), mediated by hypoxia inducible factor-1α (HIF-1α), played a crucial role in the induction of M2 macrophages by activating the signal transducer and activator of transcription 3 (STAT3)/Arginase (Arg-1) pathway. Our in vivo experiments revealed that transplanted ASCs exhibited an immunomodulatory effect by recruiting macrophages to ischemic muscle and increasing the density of M2 macrophages. The transplantation of ASCs into ischemic limbs induced increased blood flow reperfusion and limb salvage rate, whereas the depletion of tissue macrophages or transplanting HIF-1α-silenced ASCs inhibited the therapeutic effect. These findings elucidated the critical role of macrophages in ASCs-mediated ischemic muscle repair and proved that allogeneic ASCs could exert the protective effect by enhancing the recruitment of macrophages and inducing macrophages toward M2 phenotype through HIF-1α/IL-10 pathway.

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Section: Introductionmentioning

confidence: 99%

Allogeneic adipose-derived stem cells promote ischemic muscle repair by inducing M2 macrophage polarization via the HIF-1α/IL-10 pathway

et al. 2020

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“…Transplantation of allogeneic adipose-derived regenerative cells (ARCs) is also a promising treatment for ischemic diseases. Using fluorescent ARCs (GFP-ARCs) loaded with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated IONPs, Zheng et al investigated the efficacy of therapeutic angiogenesis in an ApoE −/− mouse model with hind limb ischemia [ 248 ]. Implantation of the labeled ARCs into ischemic muscles was demonstrated in vitro by immunohistochemistry and in vivo by MRI and confirmed the enhancement of neovascularization.…”

Section: Cardiovascular Tissue Regeneration and Engineeringmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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“…ADSCs have been found to improve cardiac function, attenuate anterior wall thinning, reduce fibrosis and increase peri-infarct density of both arterioles and nerve sprouts in a rat myocardial infarction model [357]. In hindlimb ischemic mouse models, ADSCs injected intravenously restored blood flow to the affected limb [138, 358,359]. The implantation of ADSCs induced angiogenesis not through endothelial differentiation of these cells, but by chemokines such as SDF-1.…”

Section: Bone Marrow (Bmscs) and Adipose Derived Stem Cells (Adscs)mentioning

confidence: 99%

Local pharmacological induction of angiogenesis: Drugs for cells and cells as drugs

Gaspar

Peixoto

Pieri

et al. 2019

Advanced Drug Delivery Reviews

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Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model

Cited by 9 publications

References 29 publications

Allogeneic adipose-derived stem cells promote ischemic muscle repair by inducing M2 macrophage polarization via the HIF-1α/IL-10 pathway

Allogeneic adipose-derived stem cells promote ischemic muscle repair by inducing M2 macrophage polarization via the HIF-1α/IL-10 pathway

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Local pharmacological induction of angiogenesis: Drugs for cells and cells as drugs

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