Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane

Xiang, Weiwei; Reglin, Bettina; Nitzsche, Bianca; Maibier, Martin; Rong, Wen Wei; Hoffmann, B.; Ruggeri, Alfredo; Guimarães, Pedro; Secomb, Timothy W.; Pries, Axel R.

doi:10.1111/micc.12351

Cited by 9 publications

(11 citation statements)

References 54 publications

(117 reference statements)

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“…23,24 After treatment with Cx blockers, a fraction of blood vessels could not be visualized anymore due to diameter reduction and impaired blood flow (see Figure 2A). The microscope (Axiotech) was equipped with two objectives (2.5×, NA 0.085; 5×, NA 0.16; Zeiss, transillumination) and CAM vascular networks were visualized for up to 24 hours.…”

Section: Intravital Microscopymentioning

confidence: 99%

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Gap junctions regulate vessel diameter in chick chorioallantoic membrane vasculature by both tone‐dependent and structural mechanisms

et al. 2019

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Objective In this study, we examined the impact of gap junction blockade on chick chorioallantoic membrane microvessels. Methods Expression of Cx37, Cx40/42, and Cx43 in chick chorioallantoic membrane tissue was studied by PCR, Western blot, and confocal immunofluorescence microscopy. Vessel diameter changes occurring under gap junction blockade with carbenoxolone (175 µmol/L), palmitoleic acid (100 µmol/L), 43GAP27 (1 mmol/L) were analyzed by intravital microscopy. To analyze vascular tone, chick chorioallantoic membrane vessels were exposed to a vasodilator cocktail consisting of acetylcholine (10 μmol/L), adenosine (100 μmol/L), papaverine (200 μmol/L), and sodium nitroprusside (10 μmol/L). Results In chick chorioallantoic membrane lysates, Western blot analysis revealed the expression of Cx40 and Cx43. Immunofluorescence in intact chick chorioallantoic membrane vasculature showed only Cx43, limited to arterial vessel walls. Upon gap junction blockade (3 hours) arterial and venous diameters decreased to 0.50 ± 0.03 and 0.36 ± 0.06 (carbenoxolone), 0.72 ± 0.08 and 0.63 ± 0.15 (palmitoleic acid) and 0.77 ± 0.004 and 0.58 ± 0.05 (GAP27), relative to initial values. Initially, diameter decrease was dominated by increasing vascular tone. After 6 hours, however, vessel tone was reduced, suggesting structural network remodeling. Conclusions Our findings suggest a major role for connexins in mediating acute and chronic diameter changes in developing vascular networks.

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Section: Intravital Microscopymentioning

confidence: 99%

“…Vessel diameters were measured using ImageJ Vs 1.51n software. 23,24 After treatment with Cx blockers, a fraction of blood vessels could not be visualized anymore due to diameter reduction and impaired blood flow (see Figure 2A). Their post-treatment diameter was arbitrarily set to 5 µm.…”

Section: Intravital Microscopymentioning

confidence: 99%

Gap junctions regulate vessel diameter in chick chorioallantoic membrane vasculature by both tone‐dependent and structural mechanisms

et al. 2019

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“…Fertilized specific pathogen-free eggs of white leghorn chicken (Gallus gallus, VALO BioMedia GmbH, Cuxhaven, Germany) were incubated at 37.5 °C and 82% humidity. On day 3 of embryonic development (ED), the eggs were opened under aseptic conditions and the content was transferred into a plastic tissue culture dish (TPP, Trasadingen, Switzerland) as described earlier [ 35 ]. The chicken embryos were then again incubated and continued developing in petri dishes until embryos reached Hamburger-Hamilton stage (HH) 36 on ED 11.…”

Section: Methodsmentioning

confidence: 99%

“…The chicken embryos were then again incubated and continued developing in petri dishes until embryos reached Hamburger-Hamilton stage (HH) 36 on ED 11. For intravital microscopy, chicken embryos were placed on a custom‐built temperature controlled (38 °C) and humidified stage [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

et al. 2021

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Angiogenesis describes the formation of new blood vessels from pre-existing vascular structures. While the most studied mode of angiogenesis is vascular sprouting, specific conditions or organs favor intussusception, i.e., the division or splitting of an existing vessel, as preferential mode of new vessel formation. In the present study, sustained (33-h) intravital microscopy of the vasculature in the chick chorioallantoic membrane (CAM) led to the hypothesis of a novel non-sprouting mode for vessel generation, which we termed “coalescent angiogenesis.” In this process, preferential flow pathways evolve from isotropic capillary meshes enclosing tissue islands. These preferential flow pathways progressively enlarge by coalescence of capillaries and elimination of internal tissue pillars, in a process that is the reverse of intussusception. Concomitantly, less perfused segments regress. In this way, an initially mesh-like capillary network is remodeled into a tree structure, while conserving vascular wall components and maintaining blood flow. Coalescent angiogenesis, thus, describes the remodeling of an initial, hemodynamically inefficient mesh structure, into a hierarchical tree structure that provides efficient convective transport, allowing for the rapid expansion of the vasculature with maintained blood supply and function during development.

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“…(In ref. 31 a similar technique might have been used to generate static images, however, these authors do not provide details of the acquisition method nor of its performances).…”

Section: Introductionmentioning

confidence: 99%

Direct imaging of capillaries reveals the mechanism of arteriovenous interlacing in the chick chorioallantoic membrane

et al. 2018

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Understanding vascular development in vertebrates is an important scientific endeavor. Normal vasculatures generally start off as a disorganized capillary lattice which progressively matures into a well-organized vascular loop comprising a hierarchy of arteries and veins. One striking feature of vascular development is the interlacing of arteries and veins. How arteries and veins manage to avoid themselves and interlace with such a perfect architecture is not understood. Here we present a detailed view of the development of the vasculature in the chorioallantoic membrane of the chicken embryo. We find that the origin of arteriovenous interlacing lies in the presence of an increased hemodynamic resistance at the distal part of the arteries due to vascular flattening onto the ectodermal surface. This reduces the vascular conductance distally, thus repelling veins away. In more proximal parts, vessels round off into cylinders and the increased flow attracts veins.

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Dynamic remodeling of arteriolar collaterals after acute occlusion in chick chorioallantoic membrane

Abstract: CAM collaterals undergo fast structural remodeling within 24 hours post-occlusion. This remodeling might be driven by local WSS and by other regulators within the vascular network.

Cited by 9 publications

References 54 publications

Gap junctions regulate vessel diameter in chick chorioallantoic membrane vasculature by both tone‐dependent and structural mechanisms

Gap junctions regulate vessel diameter in chick chorioallantoic membrane vasculature by both tone‐dependent and structural mechanisms

Coalescent angiogenesis—evidence for a novel concept of vascular network maturation

Direct imaging of capillaries reveals the mechanism of arteriovenous interlacing in the chick chorioallantoic membrane

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