Dynamics of antibodies on planar model membranes

Thompson, Nancy L.; Poglitsch, Claudia L.; Timbs, Melanie M.; Pisarchick, Mary Lee

doi:10.1021/ar00035a001

Cited by 27 publications

(20 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A common approach for studying antibody-antigen interactions has been to use phospholipid membranes containing hapten-conjugated phosphatidylethanolamine (Thompson et al, 1993). Common model haptens include dinitrophenyl (DNP), trinitrophenyl (TNP), nitroxide, dinitrophenyl nitroxide, fluorescein, and biotin.…”

Section: The Influence Of Ligand Density Of Multivalent Protein Bimentioning

confidence: 99%

Multivalent ligand–receptor binding on supported lipid bilayers

Jung

Robison

Cremer

2009

Journal of Structural Biology

View full text Add to dashboard Cite

Fluid supported lipid bilayers provide an excellent platform for studying multivalent protein-ligand interactions because the two-dimensional fluidity of the membrane allows for lateral rearrangement of ligands in order to optimize binding. Our laboratory has combined supported lipid bilayer-coated microfluidic platforms with total internal reflection fluorescence microscopy (TIRFM) to obtain equilibrium dissociation constant (K D ) data for these systems. This high throughput, on-chip approach provides highly accurate thermodynamic information about multivalent binding events while requiring only very small sample volumes. Herein, we review some of the most salient findings from these studies. In particular, increasing ligand density on the membrane surface can provide a modest enhancement or attenuation of ligand-receptor binding depending upon whether the surface ligands interact strongly with each other. Such effects, however, lead to little more than one order of magnitude change in the apparent K D values. On the other hand, the lipophilicity and presentation of lipid bilayer-conjugated ligands can have a much greater impact. Indeed, changing the way a particular ligand is conjugated to the membrane can alter the apparent K D value by at least three orders of magnitude. Such a result speaks strongly to the role of ligand availability for multivalent ligand-receptor binding.

show abstract

Section: The Influence Of Ligand Density Of Multivalent Protein Bimentioning

confidence: 99%

Multivalent ligand–receptor binding on supported lipid bilayers

Jung

Robison

Cremer

2009

Journal of Structural Biology

View full text Add to dashboard Cite

show abstract

“…Langmuir-Blodgett (LB) techniques allow preparation of ultathin films which serve as models for such biological lipid membranes on solid supports (Muller et al, 1993;Stelzle et al, 1993). Although these layers are very well characterized, their potential for the reconstitution of functional peptides and proteins is far from being fully exploited (Thompson et al, 1993;Watts et al, 1986). This paper deals with the formation of two-dimensional micro-scale stuctures on solid surfaces.…”

Section: Intr1ductkomentioning

confidence: 99%

Biologically addressable monolayer structures formed by templates of sulfur-bearing molecules

Duschl

Liley

Corradin

et al. 1994

Biophysical Journal

108

View full text Add to dashboard Cite

We demonstrate that the combined application of Langmuir-Blodgett and self-assembly techniques allows the fabrication of patterns with contrasting surface properties on gold substrates. The process is monitored using fluorescence microscopy and surface plasmon spectroscopy and microscopy. These structures are suitable for the investigation of biochemical processes at surfaces and in ultrathin films. Two examples of such processes are shown. In the first example, the structures are addressed through the binding of a monoclonal antibody to a peptide. This demonstrates the formation of self-assembled monolayers by cysteine-bearing peptides on gold, and the directed binding of proteins to the structured layers. A high contrast between specific and unspecific binding of proteins is observed by the patterned presentation of antigens. Such films possess considerable potential for the design of multichannel sensor devices. In the second example, a structured phospholipid layer is produced by controlled self-assembly from vesicle solution. The structures created--areas of phospholipid bilayer, surrounded by a matrix of phospholipid monolayer--allow formation of a supported bilayer which is robust and strongly bound to the gold support, with small areas of free-standing bilayer which very closely resemble a phospholipid cell membrane.

show abstract

“…However, while lipid conjugates and mem-nonspecific antibody interactions with the layers were invesbrane-associated proteins have a high lateral mobility in the tigated, as was the action of PIPLC. lipid layers (7,9), transmembrane species have been shown to be quasi-immobile (1,8,10), probably because of strong…”

Section: Introductionmentioning

confidence: 99%