Dynamics of vitellogenin mRNA expression and changes in hemolymph vitellogenin levels during ovarian maturation in the giant freshwater prawn <i>Macrobrachium rosenbergii</i>

Jayasankar, Vidya; Tsutsui, Naoaki; Jasmani, Safiah; Saido-Sakanaka, Hisako; Yang, Wei‐Jun; Okuno, Akira; Hien, Tran Thi; Aida, Katsumi; Wilder, Marcy N.

doi:10.1002/jez.10167

Cited by 50 publications

(27 citation statements)

References 34 publications

(9 reference statements)

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“…Dynamic levels of vitellogenin in the haemolymph were observed during reproductive activities. Determination of mRNA expression and vitellogenin levels during ovarian maturation in M. rosenbergii demonstrated a close correlation 24 . A determination of the changes of haemolymph vitellogenin levels could therefore be used to assess the action of the hormone directly 10,11 .…”

Section: Introductionmentioning

confidence: 96%

“…The sinus gland extract could reduce the vitellin mRNA expression by over 90% in the Marsupenaeus japonicus ovarian fragments in vitro 20 . Hence the ovarian vitellogenesis stage would be a suitable stage for the determination of the inhibitory effect of VIH because the change in vitellogenin mRNA expression would correlate the change in haemolymph vitellogenin levels as well 24 . In our in vivo assays, the crude eyestalk extract in M-saline at sufficient amounts (equivalent to 1/2 eyestalk) and the acetic methanol eyestalk extract (equivalent to 3 eyestalks) could effectively reduce the haemolymph vitellogenin levels in eye-ablated M. rosenbergii undergoing active ovarian development.…”

Section: Dose Responsementioning

confidence: 99%

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Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Sripiromrak¹,

Chaivisuthangkura²,

Longyant³

et al. 2014

ScienceAsia

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Vitellogenesis in crustaceans is under the inhibitory regulation of the endocrine system in the X-organ/sinus gland complex in the eyestalks. In this study, two monoclonal antibodies specific to Macrobrachium rosenbergii vitellin and vitellogenin were generated to assess vitellogenesis-inhibiting hormone (VIH) activity. A combination of two monoclonal antibodies specific to the 89 and 100 kDa subunits of vitellin were used to develop a competitive enzyme-linked immunosorbent assay protocol for the quantitative determination of vitellogenin levels in haemolymph. Injection of eyestalk extracts from M. rosenbergii dissolved in either saline or in methanol-acetic acid caused a reduction in vitellogenin levels in the haemolymph at 12 and 24 h post-injection in bilateral eye-ablated prawns with developing ovaries. In contrast, the application of the heterologous eyestalk extract from Penaeus vannamei did not reduce vitellogenin levels in haemolymph. This assay is therefore specific and could be used to assess VIH activity during hormone purification.

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Section: Introductionmentioning

confidence: 96%

Section: Dose Responsementioning

confidence: 99%

Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Sripiromrak¹,

Chaivisuthangkura²,

Longyant³

et al. 2014

ScienceAsia

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“…In recent studies, we examined the effects of eyestalk ablation on patterns of Vg synthesis during the reproductive molt cycle in adult M. rosenbergii (Okumura and Aida, 2001;Jayasankar et al, 2002). The results of these studies showed that eyestalk ablation accelerated Vg synthesis as well as ovarian maturation, but did not alter the overall pattern of Vg expression.…”

mentioning

confidence: 95%

“…The mechanisms by which these hormones regulate molting and gonadal development are complex and have been studied in a number of crustaceans (Fingerman,'87;Chang,'89;Laufer and Landau,'91;Keller,'92;De Kleijn and Van Herp,'98). Removal of the eyestalk deprives the animal of these hormones and affects various physiological processes, including ovarian development and molting (Choy, '87;Zhang et al, '97;Okumura and Aida, 2001;Jayasankar et al, 2002). Eyestalk ablation is commonly employed by aquaculturists to induce rapid ovarian maturation and subsequent spawning in prawns and shrimp in order to overcome the constraint of the unavailability of gravid females.…”

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confidence: 98%

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Dynamics of vitellogenin synthesis in juvenile giant freshwater prawn Macrobrachium rosenbergii

et al. 2006

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The dynamics of vitellogenin (Vg) mRNA expression and patterns of Vg and vitellin distribution in the hepatopancreas and ovary of juvenile Macrobrachium rosenbergii were examined using real-time RT-PCR and immunohistochemical methods. Eyestalk ablation was seen to induce rapid development of the gonads and Vg synthesis in females. In the female hepatopancreas, Vg mRNA expression was observed several days following ablation, after which levels increased gradually with increasing gonadosomatic index (GSI). Vitellin accumulation in the oocytes also increased with increasing Vg mRNA synthesis; expression was however negligible in the ovary. Hemolymph Vg levels in females ranged from 0.04 to 2.2 mg/ml. SDS PAGE/Western blotting analysis of hemolymph samples revealed that juvenile Vg was composed of 199 and 90 kDa subunits; the 102 kDa subunit present in adult female Vg (Okuno et al., 2002. J Exp Zool 292:417-429) could not be detected at any stage of vitellogenesis in juveniles. Vg was not detectable in non-ablated juveniles. The results of this study confirmed that the mode of involvement of eyestalk factors in regulating vitellogenesis is intrinsic to both juveniles and adults, and that a basic pattern of Vg synthesis and processing is conserved. However, the fact that juveniles are not able to produce the same Vg levels observed in adult females, and do not reach high GSI levels culminating in spawning suggests that other factors and physiological conditions specific to adult females are necessary to demonstrate full reproductive ability.

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Cross‐species sensitivity to a novel androgen receptor agonist of potential environmental concern, spironolactone

LaLone

Villeneuve

Cavallin

et al. 2013

Enviro Toxic and Chemistry

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Spironolactone is a pharmaceutical that in humans is used to treat conditions like hirsutism, various dermatologic afflictions, and female-pattern hair loss through antagonism of the androgen receptor. Although not routinely monitored in the environment, spironolactone has been detected downstream of a pharmaceutical manufacturer, indicating a potential for exposure of aquatic species. Furthermore, spironolactone has been reported to cause masculinization of female western mosquitofish, a response indicative of androgen receptor activation. Predictive methods to identify homologous proteins to the human and western mosquitofish androgen receptor suggest that vertebrates would be more susceptible to adverse effects mediated by chemicals like spironolactone that target the androgen receptor compared with invertebrate species that lack a relevant homolog. In addition, an adverse outcome pathway previously developed for activation of the androgen receptor suggests that androgen mimics can lead to reproductive toxicity in fish. To assess this, 21-d reproduction studies were conducted with 2 fish species, fathead minnow and Japanese medaka, and the invertebrate Daphnia magna. Spironolactone significantly reduced the fecundity of medaka and fathead minnows at 50 μg/L, whereas daphnia reproduction was not affected by concentrations as large as 500 μg/L. Phenotypic masculinization of females of both fish species was observed at 5 μg/L as evidenced by formation of tubercles in fathead minnows and papillary processes in Japanese medaka. Effects in fish occurred at concentrations below those reported in the environment. These results demonstrate how a priori knowledge of an adverse outcome pathway and the conservation of a key molecular target across vertebrates can be utilized to identify potential chemicals of concern in terms of monitoring and highlight potentially sensitive species and endpoints for testing.

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Dynamics of vitellogenin mRNA expression and changes in hemolymph vitellogenin levels during ovarian maturation in the giant freshwater prawn Macrobrachium rosenbergii

Cited by 50 publications

References 34 publications

Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii

Dynamics of vitellogenin synthesis in juvenile giant freshwater prawn Macrobrachium rosenbergii

Cross‐species sensitivity to a novel androgen receptor agonist of potential environmental concern, spironolactone

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