Dysbiosis of bifidobacteria and Clostridium cluster XIVa in the cystic fibrosis fecal microbiota

Duytschaever, Gwen; Huys, Geert; Bekaert, Maarten; Boulanger, Linda; Boeck, K. De; Vandamme, Peter

doi:10.1016/j.jcf.2012.10.003

Cited by 114 publications

(92 citation statements)

References 51 publications

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“…The observed reduction of butyrate-producing bacteria is consistent with previous findings (Duytschaever et al. 2013; Schippa et al. 2013), while no prior study has investigated acetogens presence in CF disease.…”

Section: Resultsmentioning

confidence: 99%

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Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Miragoli

Federici

Ferrari

et al. 2016

FEMS Microbiology Ecology

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Cystic fibrosis is often associated with intestinal inflammation due to several factors, including altered gut microbiota composition. In this study, we analyzed the fecal microbiota among patients with cystic fibrosis of 10–22 years of age, and compared the findings with age-matched healthy subjects. The participating patients included 14 homozygotes and 14 heterozygotes with the delF508 mutation, and 2 heterozygotes presenting non-delF508 mutations. We used PCR-DGGE and qPCR to analyze the presence of bacteria, archaea and sulfate-reducing bacteria. Overall, our findings confirmed disruption of the cystic fibrosis gut microbiota. Principal component analysis of the qPCR data revealed no differences between homozygotes and heterozygotes, while both groups were distinct from healthy subjects who showed higher biodiversity. Archaea were under the detection limit in all homozygotes subjects, whereas methanogens were detected in 62% of both cystic fibrosis heterozygotes and healthy subjects. Our qPCR results revealed a low frequency of sulfate-reducing bacteria in the homozygote (13%) and heterozygote (13%) patients with cystic fibrosis compared with healthy subjects (87.5%). This is a pioneer study showing that patients with cystic fibrosis exhibit significant reduction of H2-consuming microorganisms, which could increase hydrogen accumulation in the colon and the expulsion of this gas through non-microbial routes.

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Section: Resultsmentioning

confidence: 99%

“…Concerning CF, recent studies have examined the intestinal microbiota composition using both culture-dependent and culture-independent techniques (Duytschaever et al. 2011, 2013; Scanlan et al. 2012; Schippa et al.…”

Section: Introductionmentioning

confidence: 99%

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Miragoli

Federici

Ferrari

et al. 2016

FEMS Microbiology Ecology

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“…Indeed, the development of the mucosal immune system is dependent on the type of bacteria that are present in the lumen and that can be captured by immune cells such as dendritic cells. Previous studies have reported that fecal Bifidobacterium is reduced in patients with cystic fibrosis or with hepatitis B virus-induced chronic liver disease [38,39]. …”

Section: Discussionmentioning

confidence: 99%

Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCl<sub>4</sub>-Induced Cirrhosis

et al. 2013

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Background: The cross talk between the gut microbiota and the immune system, which is essential to maintain homeostasis, takes place at the intestinal lymphoid tissue such as the mesenteric lymph nodes (MLNs). Here, we investigated the presence of bacterial DNA in MLNs of control and cirrhotic rats and its relationship with inflammatory responses. Methods: The MLN microbiome of cirrhotic rats with ascites, which was induced by carbon tetrachloride (CCl₄), was compared to that of control rats using quantitative real-time PCR and pyrosequencing of the 16S rRNA gene. Cytokines in blood samples were assessed by ELISA. Results: Unexpectedly, sequence analysis revealed a high microbial diversity in the MLNs of both control and cirrhotic rats with Proteobacteria as one of the most dominant phylum. CCl₄-induced liver injury was not associated with a change in bacterial load, but it was linked to a decrease in microbial diversity (p < 0.05) and alterations in the microbial community in MLNs. A high proportion of Bifidobacterium animalis was also positively correlated with elevated interleukin-10 expression (p = 0.002, false discovery rate = 0.03, r = 0.94). Conclusions: For the first time, the high microbial diversity observed in MLNs of both controls and CCl₄-induced cirrhotic rats provides evidence that bacterial translocation is more than a mere dichotomic phenomenon.

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“…7,8 A cross-sectional and longitudinal comparison of the microbiota of children with CF with their healthy siblings identified instability and decreased microbiome diversity with a decrease in Clostridia, Bifidobacterium , and Veillonella in patients with CF. 38 CFTR allelic variants correlate with patterns of GI dysbiosis, and patients with homozygous ΔF508 deletion have more significant alteration in gut microbiome patterns. 39 Schippa et al 39 identified specific bacterial species related to CF disease phenotype, and that more severe disease, in addition to ΔF508 homozygosity, correlated with a predominant pattern of Escherichia coli and Escherichia biform .…”

Section: Evidence For An Altered Gut Microbiome In Cfmentioning

confidence: 96%

Neonatal Gastrointestinal and Respiratory Microbiome in Cystic Fibrosis: Potential Interactions and Implications for Systemic Health

Madan

2016

Clinical Therapeutics

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Purpose The gastrointestinal microbiome plays a critical role in nutrition and metabolic and immune functions in infants and young children and has implications for lifelong health. Cystic fibrosis (CF) transmembrane conductance regulator (CFTR) mutations in CF result in viscous mucous production, frequent exposure to antibiotics, and atypical colonization patterns, resulting in an evolving dysbiosis of the gastrointestinal and respiratory microsystems; dysbiosis in CF results in systemic inflammation, chronic infection, and dysregulation of immune function. Dysbiosis in both the respiratory system and gut contributes to undernutrition, growth failure, and long-term respiratory and systemic morbidity in infants and children with CF. Understanding the role that the gut and respiratory microbiome plays in health or disease progression in CF will afford opportunities to better identify interventions to affect clinical changes. Methods Summary was done of the pertinent literature in CF and the study of the microbiome and probiotics. Findings New studies have identified bacteria in the respiratory tract in CF that are typically members of the intestinal microbiota, and enteral exposures to breast milk and probiotics are associated with prolonged periods of respiratory stability in CF. Implications Understanding the complex interactions between the CFTR mutations, microbial colonization, and mucosal and systemic immunity is of major importance to inform new treatment strategies (such as restoring a healthier microbiome with probiotics or dietary interventions) to improve nutritional status and immune competence and to decrease morbidity and mortality in CF.

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Dysbiosis of bifidobacteria and Clostridium cluster XIVa in the cystic fibrosis fecal microbiota

Abstract: This study is the first to report specific microbial determinants of dysbiosis in patients with CF.

Cited by 114 publications

References 51 publications

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Microbiome Composition by Pyrosequencing in Mesenteric Lymph Nodes of Rats with CCl<sub>4</sub>-Induced Cirrhosis

Neonatal Gastrointestinal and Respiratory Microbiome in Cystic Fibrosis: Potential Interactions and Implications for Systemic Health

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