Dysgonomonas oryzarvi sp. nov., isolated from a microbial fuel cell

Kodama, Yoshio; Shimoyama, Takefumi; Watanabe, Kazuya

doi:10.1099/ijs.0.039040-0

Cited by 50 publications

(18 citation statements)

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“…Interestingly, the Porphyromonadaceae were 36.3% of the Bacteroidetes, and 12 isolates were similar (91%) to Parabacteroides distasonis, a human gut species that has been shown to convert aromatic amino acids to phenolic acids (41). Another 28 Porphyromonadaceae isolates were similar to Dysgonomonas mossii, which has been isolated from diseased human intestine (42) and microbial fuel cells (43); however, little is known about its function in the intestine.…”

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Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

Ziemer

2014

Appl Environ Microbiol

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One of the functions of the mammalian large intestinal microbiota is the fermentation of plant cell wall components. In ruminant animals, the majority of their nutrients are obtained via pregastric fermentation; however, up to 20% can be recovered from microbial fermentation in the large intestine. Eight-week continuous culture enrichments of cattle feces with cellulose and xylan-pectin were used to isolate bacteria from this community. A total of 459 bacterial isolates were classified phylogenetically using 16S rRNA gene sequencing. Six phyla were represented: Firmicutes (51.9%), Bacteroidetes (30.9%), Proteobacteria (11.1%), Actinobacteria (3.5%), Synergistetes (1.5%), and Fusobacteria (1.1%). The majority of bacterial isolates had <98.5% identity to cultured bacteria with sequences in the Ribosomal Database Project and thus represent new species and/or genera. Within the Firmicutes isolates, most were classified in the families Lachnospiraceae, Ruminococcaceae, Erysipelotrichaceae, and Clostridiaceae I. The majority of the Bacteroidetes were most closely related to Bacteroides thetaiotaomicron, B. ovatus, and B. xylanisolvens and members of the Porphyromonadaceae family. Many of the Firmicutes and Bacteroidetes isolates were related to species demonstrated to possess enzymes which ferment plant cell wall components; the others were hypothesized to cross-feed these bacteria. The microbial communities that arose in these enrichment cultures had broad bacterial diversity. With over 98% of the isolates not represented as previously cultured, there are new opportunities to study the genomic and metabolic capacities of these members of the complex intestinal microbiota.A function of the gut microbiota in mammalian herbivores and omnivores is the fermentation of plant cell wall components; mammals lack the enzymes required to breakdown ␤-(1,4) bonds and other linkages between the monosaccharides that make up plant cell walls (1, 2). The plant cell wall is made up of cellulose and hemicelluloses, the primary structural components of plants, and contain smaller and variable amounts of pectins, ␤-glucans, oligosaccharides, lignins, and glycoproteins (3).The physiology of ruminant animals, such as cattle, goats, and sheep, includes a foregut, where fermentation occurs and which allows the animal to directly utilize the short-chain fatty acids (SCFAs) produced by the anaerobic microbial fermentation of plant cell walls for energy (2). Thus, ruminal fermentation provides between 60% and 80% of the animal's energy requirements (4, 5). Ruminants obtain energy from the SCFAs produced in the large intestine, which provides from 0 to 20% of their dietary requirements, similar to the levels reported for other mammals (4, 5).A number of intestinal microbial metagenomes, including those of cattle (6, 7), human (8, 9), pig (10), and chicken (11), have been demonstrated to be enriched in carbohydrate transport and metabolism genes. Ley et al. (1) found that the type of diet (herbivorous, omnivorous, or carnivorous) strongly predic...

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Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

Ziemer

2014

Appl Environ Microbiol

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“…Strains of species of the genus Dysgonomonas were mostly isolated from human clinical specimens except a bioreactor of microbial fuel cell inoculated with paddy field soil (Hofstad et al, 2000;Kodama et al, 2012;Lawson et al, 2002Lawson et al, , 2010. In addition to 16S rRNA gene sequence and habitats, other distinguishing features include bile resistance, substrate utilization patterns, fatty acid composition and susceptibility to antimicrobial agents.…”

Section: Butyricimonas Synergistica Jcm 15148 T (Ab443948)mentioning

confidence: 99%

“…At the time of writing, the genus Dysgonomonas includes five species with validly published names: D. gadei, D. capnocytophagoides, D. mossii, D. hofstadii and D. oryzarvi. The first four species were isolated from human clinical specimens (Hofstad et al, 2000;Lawson et al, 2002Lawson et al, , 2010 and last one, D. oryzarvi, was from a microbial fuel cell (Kodama et al, 2012). To investigate the symbiotic roles of the gut microbiota in fungus-growing termites, we isolated bacterial strains from the hindgut of the fungus-growing termite Macrotermes barneyi.…”

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“…Reference strain D. capnocytophagoides JCM 16697 T was also characterized using the API systems in triplicate. To investigate the assimilation of single carbon sources, fermentative growth was examined in the basal peptone (BP) medium that contained the inorganic ingredients of the modified 1/10 PYGV medium, 0.5 g trypticase peptone l 21 and 10 mmol HEPES l 21 (pH 7.3) (Kodama et al, 2012). The concentration of each added carbon source was 0.5 % (w/v).…”

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“…Growth was monitored by measuring OD 600 . Utilization of different electron acceptors was tested in the BP medium supplemented with 1.8 g glucose l 21 as the sole carbon source and sodium sulfite, sodium sulfate, sodium thiosulfate, sodium nitrate, or O 2 (the head-space N 2 gas was replaced with air) as the sole electron acceptor according to the protocol of Kodama et al (2012). Organic acids were analysed by HPLC.…”

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Dysgonomonas macrotermitis sp. nov., isolated from the hindgut of a fungus-growing termite

Yang

Zhang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, facultatively anaerobic, non-motile and coccoid-to short-rod-shaped bacterium, designated strain Dys-CH1 T , was isolated from the hindgut of a fungus-growing termite Macrotermes barneyi. The optimal pH and cultivation temperature of strain Dys-CH1 T were pH 7.2-7.6 and 35-37 6C, respectively. Sequence analysis of 16S rRNA gene showed that Dys-CH1 T shared 94.6 % and 90.9 % similarity with Dysgonomonas capnocytophagoides JCM 16697 T and Dysgonomonas gadei CCUG 42882 T , respectively. Strain Dys-CH1 T was found to be different from other species of the genus Dysgonomonas with validly published names with respect to taxonomically important traits, including habitat, biochemical tests, DNA G+C content, bile resistance, fatty-acid composition and susceptibility to antimicrobial agents. On the basis of these characteristics, strain Dys-CH1 T represents a novel species of the genus Dysgonomonas for which the name Dysgonomonas macrotermitis sp. nov. is proposed. The type strain is Dys-CH1 T (5JCM 19375 T 5DSM 27370 T ).The genus Dysgonomonas, a member of the family Porphyromonadaceae in the phylum Bacteroidetes, was established by Hofstad et al., (2000). The type species of the genus is Dysgonomonas gadei (Hofstad et al., 2000). At the time of writing, the genus Dysgonomonas includes five species with validly published names: D. gadei, D. capnocytophagoides, D. mossii, D. hofstadii and D. oryzarvi. The first four species were isolated from human clinical specimens (Hofstad et al., 2000;Lawson et al., 2002Lawson et al., , 2010 and last one, D. oryzarvi, was from a microbial fuel cell (Kodama et al., 2012). To investigate the symbiotic roles of the gut microbiota in fungus-growing termites, we isolated bacterial strains from the hindgut of the fungus-growing termite Macrotermes barneyi. One strain, Dys-CH1 T , (closely related to D. capnocytophagoides JCM 16697 T , 94.6 % 16S rRNA gene sequence similarity), whose 16S rRNA gene sequence was nearly identical to uncultured bacterial clone BOf7-08 from another fungus-growing termite Odontotermes formosanus, was characterized.Strain Dys-CH1 T was isolated from the hindgut homogenate samples under strictly anaerobic conditions. The sample was serially diluted with pre-reduced 0.1 mol PBS l 21 (0.145 mol NaCl l 21 , 0.15 mol sodium phosphate l 21 ; pH 7) and spread onto modified Gifu Anaerobic Medium (GAM) agar supplemented with antibiotics (norfloxacin 40 mg ml 21 , Sigma) (Nagai et al., 2010). The plates were incubated for three days at 37 u C in anaerobic jars sealed in an anaerobic glove box (Coy Laboratory Products) which contained 88 % nitrogen, 7 % hydrogen and 5 % CO 2 . Resulting colonies were picked and further purified by restreaking on new plates, then identified preliminarily by 16S rRNA gene sequencing. Among ten investigated colonies, seven colonies were closely related to species of the genus Dysgonomonas. One colony (later named as strain Dys-CH1 T , closely related to D. capnocytophagoides JCM 16697 T ; 94.6 % 16S rRNA gene sequence simi...

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Bioelectrochemical hydrogen production with cheese whey as sole substrate

Rago

Baeza

Guisasola

2016

J of Chemical Tech & Biotech

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BACKGROUND: Microbial electrochemical systems (MXCs) are an emerging technology aiming at recovering energy contained in wastewaters either as electrical energy in microbial fuel cells (MFCs) or as hydrogen in microbial electrolysis cells (MECs).Successful results have been reported with readily biodegradable substrates, but the performance with real complex substrates needs to be evaluated to bridge the gap between lab-and full-scale. This work aims at studying bioelectrochemical hydrogen production using real cheese whey as sole substrate. RESULTS: Amicrobial consortium able to consume cheese whey to produce electricity or H 2 was developed. Cheese whey was fermented mainly by lactic acid bacteria (Enterococcus genus) and exoelectrogenic activity was performed by Geobacter sp. The coulombic efficiency was 49 ± 8% in the MFC fed only with cheese whey, which is higher than most previous values reported for MFCs fed with dairy products. Good results for H 2 production in MEC (0.8 L H2 L −1 REACTOR d −1 ) were also obtained. CONCLUSION: The high potentiality of cheese whey as carbon source for bioelectrochemical systems is demonstrated in this study. The populations involved were determined by advanced microbial tools. The efficient selection of a syntrophic consortium to produce H 2 directly from cheese whey in a single-chamber MEC was demonstrated.

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Dysgonomonas oryzarvi sp. nov., isolated from a microbial fuel cell

Cited by 50 publications

References 26 publications

Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides

Dysgonomonas macrotermitis sp. nov., isolated from the hindgut of a fungus-growing termite

Bioelectrochemical hydrogen production with cheese whey as sole substrate

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