Dysregulation of autophagy-related lncRNAs in peripheral blood of coronary artery disease patients

Ebadi, Nader; Ghafouri‐Fard, Soudeh; Taheri, Mohammad; Arsang‐Jang, Shahram; Parsa, Saeed

doi:10.1016/j.ejphar.2019.172852

“…Cycling reactions were carried out in Step One Plus Real-Time PCR equipment (Applied Biosystems, Foster city, CA, USA). Primers used for expression assays and PCR products sizes were identical to our previous studies 24,25 . Table S1 summarizes the primers features.…”

Section: Methodsmentioning

confidence: 99%

Expression of NF-κB associated lncRNAs in schizophrenia

Safa

¹

,

Badrlou

²

,

Arsang‐Jang

³

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

NF-κB signaling pathway has important roles in the regulation of growth and development of nervous system. This pathway has also been shown to participate in the pathogenesis of schizophrenia. Meanwhile, activity of NF-κB signaling pathway is regulated by several factors including non-coding RNAs (lncRNAs). In the current study, we evaluated expression of nine NF-κB-related lncRNAs namely DILC, ANRIL, PACER, CHAST, ADINR, DICER1-AS1, HNF1A-AS1, H19 and NKILA as well as two mRNA coding genes namely ATG5 and CEBPA in the peripheral blood of patients with schizophrenia compared with matched healthy subjects. Expressions of these genes were assessed by real time PCR technique. Expression of PACER was lower in patients with schizophrenia compared with controls (Posterior beta = − 0.684, P value = 0.049). On the other hand, expressions of CHAST, CEBPA, H19, HNF1A-AS1 and DICER1-AS1 were higher in patients compared with controls (Posterior beta = 0.39, P value = 0.005; Posterior beta = 0.844, P value < 0.0001; Posterior beta = 0.467, P value < 0.0001; Posterior beta = 1.107, P value = 0.005; Posterior beta = 0.176, P value = 0.044, respectively). We also appraised the diagnostic power of transcript quantities of CHAST, CEBPA, DICER1-AS1, H19 and HNF1A-AS1 in distinguishing between patients with schizophrenia and controls through depicting ROC curves. Based on the area under curve (AUC) values, CEBPA had the best diagnostic power (AUC = 0.948, P < 0.0001), followed by H19 (AUC = 0.815, P < 0.0001). Taken together, our study demonstrated dysregulation of NF-κB-related lncRNAs and genes in the peripheral blood of patients with schizophrenia and their potential as peripheral markers for this psychiatric condition.

show abstract

“…On account of their tissue and cell specificity, circulating lncRNAs are promising diagnostic markers for various diseases [15]. A previous study has identified three lncRNAs including Chast, HULC, and DICER1-AS1 that are distinctly related to autophagy in blood circulation of CAD patients [16]. Among them, HULC and DICER1-AS1 may properly differentiate CAD individuals from healthy individuals.…”

Section: Introductionmentioning

confidence: 99%

[Retracted] Dysregulated Circulating Apoptosis‐ and Autophagy‐Related lncRNAs as Diagnostic Markers in Coronary Artery Disease

Zhang

¹

,

Lou

²

,

He

³

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Objective. Increasing evidence emphasizes the implications of dysregulated apoptosis and autophagy cellular processes in coronary artery disease (CAD). Herein, we aimed to explore apoptosis- and autophagy-related long noncoding RNAs (lncRNAs) in peripheral blood of CAD patients. Methods. The mRNA and lncRNA expression profiles were retrieved from the Gene Expression Omnibus (GEO) database. With ∣ fold change ∣ > 1.5 and adjusted p value < 0.05, differentially expressed apoptosis- and autophagy-related mRNAs were screened between CAD and healthy blood samples. Also, differentially expressed lncRNAs were identified for CAD. Using the psych package, apoptosis- and autophagy-related lncRNAs were defined with Spearson’s correlation analysis. Receiver operating characteristic (ROC) curves were conducted for the assessment of the diagnosed efficacy of these apoptosis- and autophagy-related lncRNAs. Results. Our results showed that 24 apoptosis- and autophagy-related mRNAs were abnormally expressed in CAD than normal controls. 12 circulating upregulated and 1 downregulated apoptosis- and autophagy-related lncRNAs were identified for CAD. The ROCs confirmed that AC004485.3 ( AUC = 0.899 ), AC004920.3 ( AUC = 0.93 ), AJ006998.2 ( AUC = 0.776 ), H19 ( AUC = 0.943 ), RP5-902P8.10 ( AUC = 0.956 ), RP5-1114G22.2 ( AUC = 0.883 ), RP11-247A12.1 ( AUC = 0.885 ), RP11-288L9.4 ( AUC = 0.928 ), RP11-344B5.2 ( AUC = 0.858 ), RP11-452C8.1 ( AUC = 0.929 ), RP11-565A3.1 ( AUC = 0.893 ), and XXbac-B33L19.4 ( AUC = 0.932 ) exhibited good performance in differentiating CAD from healthy controls. Conclusion. Collectively, our findings proposed that circulating apoptosis- and autophagy-related lncRNAs could become underlying diagnostic markers for CAD in clinical practice.

show abstract

“…16 Moreover, it is lately reported that HULC expression is lower in peripheral blood in coronary artery disease. 17 With reference to an advanced study, lowly expressed HULC shows up in cartilage tissues in osteoarthritis. 18 Besides that, the inhibitory effects of HULC on PI3K/AKT signaling pathway have been illustrated as evidenced by decreased p-PI3K and p-AKT protein expression in AS mice.…”

Section: Discussionmentioning

confidence: 99%

“…Once suppression on the PI3K/AKT signaling pathway, activated homocysteine‐induced VSMCs proliferation and phenotypic transformation would be attenuated 16 . Moreover, it is lately reported that HULC expression is lower in peripheral blood in coronary artery disease 17 . With reference to an advanced study, lowly expressed HULC shows up in cartilage tissues in osteoarthritis 18 .…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA HULC protects against atherosclerosis via inhibition of PI3K/AKT signaling pathway

Mu

¹

,

Li

²

,

Li

³

et al. 2020

View full text Add to dashboard Cite

Studies on the roles of long non‐coding RNA in atherosclerosis (AS) have been extensively explored. However, the action of lncRNA highly upregulated in liver cancer (HULC) in AS still needs in‐depth investigations. Hence, this study is launched towards the translation of HULC‐oriented mechanism in AS. Mouse AS models were established by high cholesterol and high fat feeding. AS mice were injected with restored HULC or phosphatidylinositide 3‐kinase/protein kinase B (PI3K/AKT) signaling pathway inhibitor to explore their roles in AS. Blood lipid, inflammation and oxidative stress were detected as well as HULC, PI3K, phosphated‐PI3K (p‐PI3K), AKT, p‐AKT, and aortic vascular cell apoptosis were determined. HULC was poorly expressed, p‐PI3K and p‐AKT were highly expressed in AS. HULC inhibited the PI3K/AKT signaling pathway. In AS, by inhibition of PI3K/AKT signaling pathway, restored HULC restrained atherosclerotic plaque formation, alleviated aortic intimal damage and reduced collagen fiber content in aorta, down‐regulated blood lipid levels, and inhibited inflammation, oxidative stress and aortic vascular cell apoptosis. Our study elucidates that HULC alleviates AS via inhibition of the PI3K/AKT signaling pathway, which provides a potential biomarker for treatment of AS.

show abstract

Dysregulation of autophagy-related lncRNAs in peripheral blood of coronary artery disease patients

Cited by 16 publications

References 26 publications

Expression of NF-κB associated lncRNAs in schizophrenia

Expression of NF-κB associated lncRNAs in schizophrenia

[Retracted] Dysregulated Circulating Apoptosis‐ and Autophagy‐Related lncRNAs as Diagnostic Markers in Coronary Artery Disease

Long non‐coding RNA HULC protects against atherosclerosis via inhibition of PI3K/AKT signaling pathway

Contact Info

Product

Resources

About