Early transcriptional response of seedling roots to Ralstonia solanacearum in tobacco (Nicotiana tabacum L.)

WenXia, Gao; Chen, Renjie; Pan, Mingming; Tang, Weiqi; Lan, Tao; Huang, Likun; Chi, Wenchao; Wu, Weiren

doi:10.1007/s10658-019-01788-x

Cited by 11 publications

(10 citation statements)

References 39 publications

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“…increase in glutathione S-transferase expression was detected also in S. commersonii, however, in both Rs-resistant and Rssusceptible accessions (Zuluaga et al, 2015), while it was induced more than 10-fold in a Rs-resistant tomato cultivar in comparison with a susceptible cultivar (Ishihara et al, 2012). Transcriptome analysis of seedling roots of an Rs-resistant tobacco cultivar suggested that glutathione and flavonoids are probably the main substances conferring early resistance against Rs infection (Gao et al, 2019). This suggestion was supported by Li et al (2021), who demonstrated that glutathione metabolism and phenylpropanoid pathways are the primary resistance pathways to Rs infection in tobacco.…”

Section: Rs Infectionmentioning

confidence: 99%

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

et al. 2023

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Ralstonia solanacearum (Rs), the causal agent of bacterial wilt disease in an unusually wide range of host plants, including potato (Solanum tuberosum), is one of the most destructive phytopathogens that seriously reduces crop yields worldwide. Identification of defence mechanisms underlying bacterial wilt resistance is a prerequisite for biotechnological approaches to resistance breeding. Resistance to Rs has been reported only in a few potato landraces and cultivars. Our in vitro inoculation bioassays confirmed that the cultivars ‘Calalo Gaspar’ (CG) and ‘Cruza 148’ (CR) are resistant to Rs infection. Comparative transcriptome analyses of CG and CR roots, as well as of the roots of an Rs-susceptible cultivar, ‘Désirée’ (DES), were carried out two days after Rs infection, in parallel with their respective noninfected controls. In CR and DES, the upregulation of chitin interactions and cell wall-related genes was detected. The phenylpropanoid biosynthesis and glutathione metabolism pathways were induced only in CR, as confirmed by high levels of lignification over the whole stele in CR roots six days after Rs infection. At the same time, Rs infection greatly increased the concentrations of chlorogenic acid and quercetin derivatives in CG roots as it was detected using ultra-performance liquid chromatography − tandem mass spectrometry. Characteristic increases in the expression of MAP kinase signalling pathway genes and in the concentrations of jasmonic, salicylic, abscisic and indoleacetic acid were measured in DES roots. These results indicate different Rs defence mechanisms in the two resistant potato cultivars and a different response to Rs infection in the susceptible cultivar.

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Section: Rs Infectionmentioning

confidence: 99%

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

et al. 2023

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“…A total of 75 tobacco seedlings were chosen and inoculated with a highly virulent pathogenic strain of Ras. that had been isolated and maintained by our laboratory [41]. Inoculation was performed by mechanically wounding the roots and irrigating with 30 mL of Ras.…”

Section: Expression Analysis Of Ntadh Genes In To Ralstonia Solanacearummentioning

confidence: 99%

Genome-wide Identification and Expression Analysis of the ADH Gene Family in Tobacco (Nicotiana tabacum L.)

Wang,

Du,

et al. 2023

Preprint

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Background Alcohol dehydrogenases (ADHs) are the crucial enzymes involved in plant development, biotic and abiotic stresses. Tobacco is one of the most important model plants, which will be of great application significance to analyze the ADH gene family and expression profile under various stresses in tobacco. Results A total of 53 ADH genes were identified in tobacco (Nicotiana tabacum L.) genome and were grouped into 6 subfamilies based on phylogenetic analysis. Gene structure (exon/intron) and protein motifs were highly conserved among the NtADH genes, especially the members within the same subfamily. A total of 5 gene pairs of tandem duplication, and 3 gene pairs of segmental duplication were identified based on the analysis of gene duplication events. Cis-regulatory elements of the NtADH promoters participated in cell development, plant hormones, environmental stress, and light responsiveness. Expression profile analysis showed that NtADH genes were widely expressed in different maturity tobacco leaves, as well as in leaves after topping, however, the expression patterns of different members appeared to be diversed. The qRT-PCR analysis of 13 NtADH genes confirmed their differential expression in response to the bacterial pathogen Ralstonia solanacearum L. infection. Metabolomics analysis revealed that NtADH genes were primarily associated with carbohydrate metabolism, and moreover, four NtADH genes (NtADH20/24/48/51) were notably involved in the pathway of alpha-linolenic acid metabolism. Conclusion The genome-wide identification, evolutionary and expression analysis of NtADH genes have provided valuable insights into the role of these genes in response to abiotic and biotic stresses. Our results have also laid a solid foundation for further functional study on ADH genes in tobacco, especially for the understanding of NtADHs under more stress conditions.

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“…Transcriptomic analysis using RNA-seq has been used to profile host gene expression during bacterial wilt disease (Chen et al, 2014(Chen et al, , 2018French et al, 2018;Gao et al, 2019;Ghareeb et al, 2011;Jiang et al, 2019;Prasath et al, 2014;Zuluaga et al, 2015). Although these studies have shed light on the disease, they have limitations.…”

Section: Introductionmentioning

confidence: 99%

Tomato deploys defence and growth simultaneously to resist bacterial wilt disease

Méline

Hendrich

Truchon

et al. 2022

Plant Cell & Environment

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Plant disease limits crop production, and host genetic resistance is a major means of control. Plant pathogenic Ralstonia causes bacterial wilt disease and is best controlled with resistant varieties. Tomato wilt resistance is multigenic, yet the mechanisms of resistance remain largely unknown. We combined metaRNAseq analysis and functional experiments to identify core Ralstonia‐responsive genes and the corresponding biological mechanisms in wilt‐resistant and wilt‐susceptible tomatoes. While trade‐offs between growth and defence are common in plants, wilt‐resistant plants activated both defence responses and growth processes. Measurements of innate immunity and growth, including reactive oxygen species production and root system growth, respectively, validated that resistant plants executed defence‐related processes at the same time they increased root growth. In contrast, in wilt‐susceptible plants roots senesced and root surface area declined following Ralstonia inoculation. Wilt‐resistant plants repressed genes predicted to negatively regulate water stress tolerance, while susceptible plants repressed genes predicted to promote water stress tolerance. Our results suggest that wilt‐resistant plants can simultaneously promote growth and defence by investing in resources that act in both processes. Infected susceptible plants activate defences, but fail to grow and so succumb to Ralstonia, likely because they cannot tolerate the water stress induced by vascular wilt.

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Early transcriptional response of seedling roots to Ralstonia solanacearum in tobacco (Nicotiana tabacum L.)

Cited by 11 publications

References 39 publications

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

Genome-wide Identification and Expression Analysis of the ADH Gene Family in Tobacco (Nicotiana tabacum L.)

Tomato deploys defence and growth simultaneously to resist bacterial wilt disease

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