Easy One-Pot Low-Temperature Synthesized Ag-ZnO Nanoparticles and Their Activity Against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Abstract

Antimicrobial resistance (AMR) is widely acknowledged as a global health problem, yet the available solutions to this problem are limited. Nanomaterials can be used as potential nanoweapons to fight against this problem. In this study, we report an easy one-pot low-temperature synthesis of Ag-ZnO nanoparticles (AZO NPs) and their targeted antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) strains. The physical properties of the samples were characterized by X-ray diffractometry (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). Furthermore, minimum inhibitory concentration (MIC), zone of inhibition (ZOI), and scanning electron microscopy (SEM) images for morphological characterization of bacteria were assessed to evaluate the antibacterial activity of AZO NPs against both Gram-negative [Escherichia coli (E. coli) and Acinetobacter baumannii (A. baumannii) standard and AMR strains] and Gram-positive (S. aureus, MRSA3, and MRSA6) bacteria. The AZO NPs showed comparatively better antibacterial activity against S. aureus and MRSA strains than Gram-negative bacterial strains. This costeffective and simple synthesis strategy can be used for the development of other metal oxide nanoparticles, and the synthesized nanomaterials can be potentially used to fight against MRSA.

Recent Advances in Nanomaterial-Based Wound-Healing Therapeutics

“…Typically, the size, shape, surface functionalization, zeta potential, porosity, and hydrolytic stability of metallic nanoparticles affect the efficacy of nanomaterials in biological applications [16][17][18][19]. Among metallic nanoparticles, the most researched ones are silver (Ag) [83], gold (Au) [84], and zinc oxide (ZnO) [85] owing to their favorable physiochemical properties and antibacterial activity.…”

A New Nano-Platform of Erythromycin Combined with Ag Nano-Particle ZnO Nano-Structure against Methicillin-Resistant Staphylococcus aureus

“…Notably, bacteria can reduce their interactions with antibiotics to result in drug resistance [22]. However, the antibacterial mechanism of NPs [6,7] or, in this case, AZO NPs mainly involves membrane disruption of bacteria which occurs independently of direct interactions with bacterial components. Therefore, AZO NP may overcome resistance to antibiotics.…”

“…Because of their antibacterial activities, various metal and metal oxide nano-particles such as ZnO, CuO, TiO 2 , SiO 2 , MgO, Cao, Ag, Au, and Cu offer effective solutions for treating infections by MDR pathogens [5]. ZnO has been widely used by researchers because of its antibacterial activity and distinctive physiochemical properties such as high surface area-to-volume ratio [6,7]. The Food and Drug Administration has already recognized ZnO as a "GRAS" (generally recognized as safe) substance (FDA, 21CFR182.8991, USA) [8].…”

Potential Novel Food-Related and Biomedical Applications of Nanomaterials Combined with Bacteriocins

“…Currently, metallic nanoparticles such as zinc, copper, silver, and gold are being studied not only for their antibacterial activity, but also for their different potential biomedical applications [ 12 , 13 , 57 , 58 , 59 , 60 ]. The extensive use of these nanoparticles can be attributed to their large surface area along with their positive charge, which can interact with negatively charged bacterial cell surfaces [ 11 ].…”