Ecological Balance of Oral Microbiota Is Required to Maintain Oral Mesenchymal Stem Cell Homeostasis

Su, Yingying; Chen, Chider; Guo, Lijia; Du, Juan; Li, Xiaoyan; Liu, Yi

doi:10.1002/stem.2762

Cited by 40 publications

(33 citation statements)

References 55 publications

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“…An antibiotic cocktail of 1300 mg/l of metronidazole and 660 mg/l of levofloxacin in the drinking water was used to remove oral bacteria in mice for 2 weeks. After that, the bacteria of oral mucosa of mice were scraped with cotton swab and analyzed as previously [22]. UCLUST was used to divide the unique sequence set into operational classifiers (OTUs) under 97% recognition threshold.…”

Section: S Rrna Sequencing and Analysismentioning

confidence: 99%

“…Mesenchymal stem cells (MSCs) are considered as a promising method that has the potential to promote tissue regeneration and wound healing due to the multilineage differentiation and self-renewal properties [18][19][20][21]. Our previous study found that in an environment of oral microecological imbalance, the function of oral gingival and palatal MSCs was impaired, which played an important role in the repair of oral soft tissue damage, and the healing speed of oral soft tissue was significantly slowed down [22]. However, the effects of the balance between oral pathogenic bacteria and probiotics on the physiological function of MSCs and wound healing are still unclear.…”

Section: Introductionmentioning

confidence: 99%

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Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis

et al. 2020

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Objectives: The homeostasis of oral pathogenic bacteria and probiotics plays a crucial role in maintaining the well-being and healthy status of human host. Our previous study confirmed that imbalanced oral microbiota could impair mesenchymal stem cell (MSC) proliferation capacity and delay wound healing. However, the effects of balanced oral pathogenic bacteria and probiotics on MSCs and wound healing are far from clear. Here, the balance of pathogenic bacteria Porphyromonas gingivalis and probiotics Lactobacillus reuteri extracts was used to investigate whether balanced oral microbiota modulate the physiological functions of MSCs and promote wound healing. Methods: The effects of balanced pathogenic bacteria P. gingivalis and probiotics L. reuteri extracts on gingival MSCs (GMSCs) were tested using the migration, alkaline phosphatase activity, alizarin red staining, cell counting kit-8, realtime PCR, and western blot assays. To investigate the role of balanced pathogenic bacteria P. gingivalis and probiotics L. reuteri extracts in the wound of mice, the wounds were established in the mucosa of palate and were inoculated with bacteria every 2 days. Results: We found that the balance between pathogenic bacteria and probiotics enhanced the migration, osteogenic differentiation, and cell proliferation of MSCs. Additionally, local inoculation of the mixture of L. reuteri and P. gingivalis promoted the process of wound healing in mice. Mechanistically, we found that LPS in P. gingivalis could activate NLRP3 inflammasome and inhibit function of MSCs, thereby accelerating MSC dysfunction and delaying wound healing. Furthermore, we also found that reuterin was the effective ingredient in L. reuteri which maintained the balance of pathogenic bacteria and probiotics by neutralizing LPS in P. gingivalis, thus inhibiting inflammation and promoting wound healing. Conclusions: This study revealed that the homeostasis of oral microbiomes played an indispensable role in maintaining oral heath, provided hopeful methods for the prevention and treatment of oral diseases, and had some referential value for other systemic diseases caused by dysfunction of microbiota and MSCs.

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Section: S Rrna Sequencing and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis

et al. 2020

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“…Many factors can disturb the functions of GMSCs. Su et al reported disturbed oral microbiome weakened the wound healing of GMSCs through miR-21/Sp1/telomerase reverse transcriptase pathway [66]. The physical condition of donors is a key factor to properties of GMSCs.…”

Section: Analysis Of Factors Influencing the Function Of Gmscmentioning

confidence: 99%

GMSC: Updates of Advances on Its Therapy in Immunological Diseases

Hou¹,

Zheng²

2020

Update on Mesenchymal and Induced Pluripotent Stem Cells

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Mesenchymal stem cells (MSCs) derived from various tissues are multi-potency of self-renewal and differentiation into multi-lineages, including chondrocytes, adipocytes and osteoblasts in vitro and in vivo. In addition, these cells also display potent immune regulatory roles that benefit the treatment of inflammatory and autoimmune diseases. We and others have previously identified that human gingival-derived mesenchymal stem cells (GMSCs) not only share similar biological features, but also display some potential advantages compared to other MSC populations. In the chapter, we have discussed the discovery, phenotypic and functional characteristics, as well as updated the advances of these cell therapies in immunological diseases.

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“…Even healthy people can present cariogenic bacteria [ 2 , 3 ]. It is important to maintain the balance of oral microbiome for oral and body health [ 4 , 5 ]. Dental caries is one of the most widespread and costly biofilm-mediated oral infectious diseases, affecting all ages of people worldwide [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Regulating Oral Biofilm from Cariogenic State to Non-Cariogenic State via Novel Combination of Bioactive Therapeutic Composite and Gene-Knockout

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The objectives were to investigate a novel combination of gene-knockout with antimicrobial dimethylaminohexadecyl methacrylate (DMAHDM) composite in regulating oral biofilm from a cariogenic state toward a non-cariogenic state. A tri-species biofilm model included cariogenic Streptococcus mutans (S. mutans), and non-cariogenic Streptococcus sanguinis (S. sanguinis) and Streptococcus gordonii (S. gordonii). Biofilm colony-forming-units (CFUs), lactic acid and polysaccharide production were measured. TaqMan real-time-polymerase-chain reaction was used to determine the percentage of each species in biofilm. The rnc gene-knockout for S. mutans with DMAHDM composite reduced biofilm CFU by five logs, compared to control (p < 0.05). Using parent S. mutans, an overwhelming S. mutans percentage of 68.99% and 69.00% existed in biofilms on commercial composite and 0% DMAHDM composite, respectively. In sharp contrast, with a combination of S. mutans rnc knockout and DMAHDM composite, the cariogenic S. mutans percentage in biofilm was reduced to only 6.33%. Meanwhile, the non-cariogenic S. sanguinis + S. gordonii percentage was increased to 93.67%. Therefore, combining rnc-knockout with bioactive and therapeutic dental composite achieved the greatest reduction in S. mutans, and the greatest increase in non-cariogenic species, thereby yielding the least lactic acid-production. This novel method is promising to obtain wide applications to regulate biofilms and inhibit dental caries.

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Ecological Balance of Oral Microbiota Is Required to Maintain Oral Mesenchymal Stem Cell Homeostasis

Cited by 40 publications

References 55 publications

Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis

Balanced oral pathogenic bacteria and probiotics promoted wound healing via maintaining mesenchymal stem cell homeostasis

GMSC: Updates of Advances on Its Therapy in Immunological Diseases

Regulating Oral Biofilm from Cariogenic State to Non-Cariogenic State via Novel Combination of Bioactive Therapeutic Composite and Gene-Knockout

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