Ecotoxicological Assessment of Aquatic Genotoxicity Using the Comet Assay

Yaqin, Khusnul

doi:10.1016/s1978-3019(16)30305-9

Cited by 4 publications

(4 citation statements)

References 62 publications

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“…Despite all mentioned above, there are few studies assessing genotoxicity on microalgae. In the literature, the most common organisms which were used as sentinel organisms in aquatic eco-genotoxicology, were fish, followed by mussel and amphibian (Yaqin 2006). There are few works in which genotoxicity is evaluated on unicellular algae (Erbes et al 1997;Sastre et al 2001;Aoyama et al 2003;Desai et al 2006;Akcha et al 2008;Prado et al 2009), being single-celled, they can be used as a model for assessment of DNA damage and monitoring the environmental impact of xenobiotics compounds (Cotelle and Fe ´rard 1999;Dhawan et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Genotoxic effects of commercial formulations of Chlorpyrifos and Tebuconazole on green algae

2014

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The alkaline single-cell gel electrophoresis assay (comet assay) was used for the study of the genotoxic effects of insecticide Chlorpyrifos and fungicide Tebuconazole (commercial formulations) on two freshwater green algae species, Pseudokirchneriella subcapitata and Nannocloris oculata, after 24 h of exposure. The percentage of DNA in tail of migrating nucleoids was taken as an endpoint of DNA impairment. Cell viability was measured by fluorometric detection of chlorophyll "a" in vivo and the determination of cell auto-fluorescence. Only the higher concentration of Chlorpyrifos tested resulted to affect significantly the cell viability of P. subcapitata, whereas cells of N. oculata were not affected. Tebuconazole assayed concentrations (3 and 6 mg/l) did not affect cell viability of both species. The results of comet assay on P. subcapitata showed that Chlorpyrifos concentration evaluated (0.8 mg/l) exerted a genotoxic effects; while for the other specie a concentration of 10 mg/l was needed. Tebuconazole was genotoxic at 3 and 6 mg/l for both species. The comet assay evidenced damage at the level of DNA simple strains molecule at pesticide concentrations were cytotoxicity was not evident, demonstrating that algae are models to take into account in ecological risk assessments for aquatic environments.

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Section: Introductionmentioning

confidence: 99%

Genotoxic effects of commercial formulations of Chlorpyrifos and Tebuconazole on green algae

2014

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“…Further, the fish blood with excellent cellular homogeneity (97% being erythrocytes) is considered ideal for comet assay experiments [7]. The use of comet assay has also proven in other studies, examining such topics as the acute and chronic genotoxicity of oxytetracycline in rainbow trout [22][23][24]. Consistent with a previous report, our results suggest that the pathologic and genotoxic effects of M. officinalis extract are linked to the dose and duration of exposure (Tables 1-3) [25].…”

Section: Comet Assaymentioning

confidence: 99%

Genotoxicity and Histopathology Effects of Melissa officinalis Aqueous Extract on the Blood and Vital Tissues of Oncorhynchus mykiss Fish

Jafarpour

Amniattalab

Nekuiefard

2018

IJT

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Background: This study was conducted to investigate both the genotoxicity effects of M. officinalis aqueous extract on blood cells and the pathologic changes in the renal, cardiac and splenic tissues of Oncorhynchus mykiss. Methods: 300 fish (Oncorhynchus mykiss) were divided randomly into three groups (N=100 each), consisting of group, 1 (control), and groups 2 and 3 (experimental), which received 450 mg/kg and 1350 mg/kg of body weight the aqueous extract of M. officinalis, respectively. The fish were fed for 30 days, with the experimental groups given three treatments. Micronuclei test and comet assay were used to identify the histopathological damages, simultaneously. Results: We found significantly more micronuclei (33%) in erythrocytes of group 3 than those in group 2 (5%; p<0.05). Similarly, the results of comet assay were consistent with those obtained for the micronuclei test. The recorded DNA damages to erythrocytes was significantly higher in group 3 (35.75%) compared to that for group 2 (7.15%; p<0.05). The pathologic findings in the spleen, kidneys and heart tissues together with those obtained from the micronuclei test and comet assay confirmed the tissue and DNA damages after exposure to the extract. Abundant and severe cystic and atrophic glomeruli, renal tubular degeneration, hemorrhage and focal lymphocytic inflammation in heart, and increased melanomacrophage centers in the kidneys and spleen were observed at significantly higher frequency in group 3 than those in group 2 (p<0.05). Conclusion: The findings of this study demonstrated that the extract of M. officinalis at doses higher than 450 mg/kg per body weight caused toxic effects with severe tissue and DNA damages in O. mykiss fish.

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“…(Bolognesi et al, 1996;Lee et al, 2020;Qin et al, 2020;Baines et al, 2021). They have significant influences in cell damage creating necrosis and neoplasia (Bebianno & Garcia da Fonseca, 2020;Medda et al, 2021), DNA damage (Yaqin, 2006;Asllani et al, 2019;Bolognesi et al, 2019;Pellegri et al, 2020;Guimarães et al, 2021;Camargo Filho et al, 2022), embryonic mortality and mutation (McLaughlin et al, 2020;Ganaie, 2021) and Darwinian fitness such as decrease of growth rates, reproductive output, and viability of offspring (Depledge, 2020).…”

Section: Introductionmentioning

confidence: 99%

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

Yaqin

2022

Akuatikisle: J. Aqua. Coast. & Isle

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Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour. The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters. DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro. The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site. Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.

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Ecotoxicological Assessment of Aquatic Genotoxicity Using the Comet Assay

Cited by 4 publications

References 62 publications

Genotoxic effects of commercial formulations of Chlorpyrifos and Tebuconazole on green algae

Genotoxic effects of commercial formulations of Chlorpyrifos and Tebuconazole on green algae

Genotoxicity and Histopathology Effects of Melissa officinalis Aqueous Extract on the Blood and Vital Tissues of Oncorhynchus mykiss Fish

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

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