Ecotoxicological effects of an aged TiO2 nanocomposite measured as apoptosis in the anecic earthworm Lumbricus terrestris after exposure through water, food and soil

Lapied, Emmanuel; Nahmani, Johanne; Moudilou, Elara; Chaurand, Perrine; Labille, Jérôme; Rose, Jérôme; Exbrayat, Jean-Marie; Oughton, Deborah; Joner, Erik J.

doi:10.1016/j.envint.2011.01.009

Cited by 94 publications

(47 citation statements)

References 36 publications

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“…Bioaccumulation of TiO 2 NPs was not observed in the earthworm Lumbricus terrestris when exposed via food, soil, and water [94]. Although apoptosis could be observed in the most exposed tissues (e.g., cuticle and intestine), the NPs did not pass the tissue barrier, which prevented bioaccumulation [94].…”

Section: Titanium Dioxidementioning

confidence: 95%

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Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

Tourinho

Gestel²,

Lofts

et al. 2012

Enviro Toxic and Chemistry

401

229

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Abstract-Metal-based nanoparticles (NPs) (e.g., silver, zinc oxide, titanium dioxide, iron oxide) are being widely used in the nanotechnology industry. Because of the release of particles from NP-containing products, it is likely that NPs will enter the soil compartment, especially through land application of sewage sludge derived from wastewater treatment. This review presents an overview of the literature dealing with the fate and effects of metal-based NPs in soil. In the environment, the characteristics of NPs (e.g., size, shape, surface charge) and soil (e.g., pH, ionic strength, organic matter, and clay content) will affect physical and chemical processes, resulting in NP dissolution, agglomeration, and aggregation. The behavior of NPs in soil will control their mobility and their bioavailability to soil organisms. Consequently, exposure characterization in ecotoxicological studies should obtain as much information as possible about dissolution, agglomeration, and aggregation processes. Comparing existing studies is a challenging task, because no standards exist for toxicity tests with NPs. In many cases, the reporting of associated characterization data is sparse, or missing, making it impossible to interpret and explain observed differences in results among studies. Environ. Toxicol. Chem.

show abstract

Section: Titanium Dioxidementioning

confidence: 95%

“…Although apoptosis could be observed in the most exposed tissues (e.g., cuticle and intestine), the NPs did not pass the tissue barrier, which prevented bioaccumulation [94].…”

Section: Titanium Dioxidementioning

confidence: 97%

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

Tourinho

Gestel²,

Lofts

et al. 2012

Enviro Toxic and Chemistry

401

229

View full text Add to dashboard Cite

show abstract

“…They can be exposed to pollutants entering the soil through both dermal contact and ingestion [9]. Currently, in both types of coelomocyte populations, amoebocytes are more responsive and persuasive for all mitotic figures observed in terms of oxidative stress, cyto-toxicity and geno-toxicity tests of soil pollutants [10].…”

Section: Introductionmentioning

confidence: 99%

“…Earthworms have been widely used in the investigation of adverse effects of persistent organic pollutants (POPs), and recently of effects of PAEs in natural soil to Eisenia fetida [10, 15]. Enhanced frequency of apoptosis has been employed in previous studies on organisms including earthworms with satisfactory results [9, 16]. Other popular biomarkers including neutral red retention time (NRRT), metallothionein (MT) and heat shock proteins (HSP 70) are usually chosen to describe the mechanisms by which pollutants damage target earthworms in tests using spiked soil [17, 18].…”

Section: Introductionmentioning

confidence: 99%

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Zhou

Chen

et al. 2017

PLoS ONE

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Di-(2-ethylhexyl) phthalate (DEHP) is a dominant phthalic acid ester (PAE) that has aroused public concern due to its resistance to degradation and its toxicity as an endocrine-disrupting compound. Effects of different concentrations of DEHP on Eisenia fetida in spiked natural soil have been studied in the body of the earthworm by means of soil cultivation tests 7, 14, 21 and 28 days after exposure. The results indicated that, in general, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, metallothionein (MT) content, the expression of heat shock protein 70 (HSP 70) and all the tested geno-toxicity parameters are promoted as time elapses and with increasing concentration of DEHP. However, peroxidase (POD) activity, neutral red retention time (NRRT) and mitochondrial membrane potential difference values were found to decrease even at a low concentration of DEHP of 1 mg kg-1 soil (p<0.05). Clear toxic effects of DEHP on E. fetida have been generally recognized by means of the disturbance of antioxidant enzyme activity/content and critical proteins, cell membrane and organelle disorder and DNA damage estimated by length of tail, tail DNA ratio, and tail moment parameters. A concentration of DEHP of 3 mg kg-1 may be recommended as a precaution against the potential risk of PAEs in soils and for indicating suitable threshold values for other soil animals and soil micro-organisms.

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“…In a study dealing with aged TiO 2 composites, the apoptotic frequency appears to be more sensitive to TiO 2 nanoparticles than a conventional endpoint (mortality). Aged TiO 2 composites did not induce mortality in the earthworm Lumbricus terrestris up to the highest test concentration (100 mg/kg), but the apoptotic frequency increased [24]. Fresh (non-aged) material was not studied at the same time, so the influence of aging cannot be determined.…”

Section: Ecotoxicity Of Tio 2 and Influence Of Substance Propertiesmentioning

confidence: 99%

Effect of TiO2 nanoparticles in the earthworm reproduction test

Schlich

Terytze²,

Hund‐Rinke

2012

Environ Sci Eur

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Background: The increasing use of nanotechnology means that nanomaterials will enter the environment. Ecotoxicological data are therefore required so that adequate risk assessments can be carried out. In this study, we used a standardized earthworm reproduction test with Eisenia andrei to evaluate three types of TiO 2 nanoparticles (NM-101, NM-102, NM-103). The test was performed in natural sandy soil (RefeSol 01A) following Organisation for Economic Co-operation and Development Test Guideline No. 222. The nanoparticles differed in several aspects, such as crystalline structure, size, and the presence or absence of a coating. Results: Uncoated nanoparticles stimulated earthworm reproduction in a concentration-dependent manner during winter testing, increasing the number of offspring by up to 50% compared to the control. However, there was no stimulation when the same test was performed in the summer. This reflected an underlying circannual rhythm observed in the control soil, characterized by the production of a significantly larger number of juveniles in summer compared with that in winter. The effect of the uncoated TiO 2 nanoparticles was to reduce or eliminate the circannual differences by increasing the reproductive rate in winter. Coated TiO 2 nanoparticles did not influence earthworm reproduction. Conclusion: TiO 2 appears to affect earthworm reproductive activity by abolishing the circannual rhythm that depresses reproduction in the winter. Further experiments will be necessary to determine (1) the mode of action of the nanoparticles, (2) the important parameters causing the effect (e.g., relevant soil parameters), and (3) the environmental relevance of continuous earthworm reproduction we observed under laboratory conditions.

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Ecotoxicological effects of an aged TiO2 nanocomposite measured as apoptosis in the anecic earthworm Lumbricus terrestris after exposure through water, food and soil

Cited by 94 publications

References 36 publications

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

Metal‐based nanoparticles in soil: Fate, behavior, and effects on soil invertebrates

Toxicity effects of di-(2-ethylhexyl) phthalate to Eisenia fetida at enzyme, cellular and genetic levels

Effect of TiO2 nanoparticles in the earthworm reproduction test

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