Ectopic expression of bacterial 1-aminocyclopropane 1-carboxylate deaminase in Chlamydomonas reinhardtii enhances algal biomass and lipid content under nitrogen deficit condition

Srinivasan, R.; Subramanian, Parthiban; Tirumani, Srikanth; Gothandam, Kodiveri Muthukaliannan; Ramya, Mohandass

doi:10.1016/j.biortech.2021.125830

Cited by 5 publications

(3 citation statements)

References 50 publications

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“…IAA enhances plant growth and can increase the rate of nitrogen fixation of endophytic bacteria ( Defez, Andreozzi & Bianco, 2017 ). ACC deaminase (ACCD) positive bacterial strains are present in Rhodococcus ( Kukla, Plociniczak & Piotrowska-Seget, 2014 ) and Polaromonas ( Jasim et al, 2015 ), which can significantly reduce reactive oxygen species (ROS) and lipid peroxidation levels ( Srinivasan et al, 2021 ). In addition, ACCD can substantially increase the host’s biomass, chlorophyll content, and photosynthetic activity in low-N conditions ( Srinivasan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions

Xia

et al. 2023

PeerJ

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Background Leymus secalinus is a pioneer plant grown in the Zoige desertified alpine grassland and it is also one of the dominant plant species used for environmental remediation. L. secalinus plays a large role in vegetation reconstruction in sandy land, but the abundance and diversity of its endophytes have not yet been investigated. Objectives This study was performed to investigate the changes in the endophytic bacterial community structure of L. secalinus under different ecological environments and to analyze the effects of environmental changes and different plant tissues on the L. secalinus endophytic bacteria. Methods Leaf, stem, and root tissue samples of L. secalinus were collected from Zoige Glassland (Alpine sandy land) and an open field nursery (Control). DNA was extracted and the 16S ribosomal DNA was amplified. The sequence library was sequenced on an Illumina MiSeq platform and clustered by operational taxonomic units (OTUs). α-diversity and β-diversity analyses, species diversity analyses, functional prediction, and redundancy (RDA) analyses for the soil physicochemical properties were conducted. Results α-diversity and β-diversity analyses showed that the endophytic bacteria in L. secalinus varied in different areas and tissues. The abundance of Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, which is related to nitrogen fixation, increased significantly in the L. secalinus found in the Zoige Grassland. Moreover, the abundance of nutrition metabolism and anti-stress abilities increased in functional prediction in the desert samples. The soil physicochemical properties had an insignificant influence on bacterial diversity. Conclusion The changes in the endophytic bacterial community structure in L. secalinus were significant and were caused by environmental alterations and plant choice. The endophytic bacteria in L. secalinus grown in alpine sandy land may have greater anti-stress properties and the ability to fix nitrogen, which has potential value in environmental remediation and agricultural production.

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Section: Discussionmentioning

confidence: 99%

The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions

Xia

et al. 2023

PeerJ

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“…ACC-deaminase-producing PGPR has been considered in recent years an effective plant probiotic in plant roots. Several studies [ 22 , 23 , 24 , 38 , 39 , 40 , 55 , 56 , 57 , 58 , 59 ] have shown that bacteria that produce ACC deaminase and are introduced to the rhizosphere of plants can decrease the amount of ethylene produced by plants under stress. This reduction helps plants resist stress while also promoting growth by using the produced α-ketobutyrate and ammonia.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, these bacteria can utilize the α-ketobutyrate and ammonia produced by the plant to promote growth. In recent years, more and more researchers have introduced the acdS gene, i.e., the gene encoding ACC deaminase, into plant species to give them the ability to produce ACC deaminase, thereby resisting various abiotic stresses and promoting plant growth and development [ 38 , 39 , 40 ]. Therefore, ACC-deaminase-producing bacteria can be used as a green method to control ethylene produced by stress.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Diversity Analysis and Screening for ACC Deaminase-Producing Strains in Moss-Covered Soil at Different Altitudes in Tianshan Mountains—A Case Study of Glacier No. 1

et al. 2023

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The elevation of the snowline of the No. 1 Glacier in the Tianshan Mountains is increasing due to global warming, which has created favorable conditions for moss invasion and offers an opportunity to investigate the synergistic effects of incipient succession by mosses, plants, and soils. In this study, the concept of altitude distance was used instead of succession time. To investigate the changes of bacterial-community diversity in moss-covered soils during glacial degeneration, the relationship between bacterial community structure and environmental factors was analyzed and valuable microorganisms in moss-covered soils were explored. To do so, the determination of soil physicochemical properties, high-throughput sequencing, the screening of ACC-deaminase-producing bacteria, and the determination of ACC-deaminase activity of strains were performed on five moss-covered soils at different elevations. The results showed that the soil total potassium content, soil available phosphorus content, soil available potassium content, and soil organic-matter content of the AY3550 sample belt were significantly different compared with those of other sample belts (p < 0.05). Secondly, there was a significant difference (p < 0.05) in the ACE index or Chao1 index between the moss-covered-soil AY3550 sample-belt and the AY3750 sample-belt bacterial communities as the succession progressed. The results of PCA analysis, RDA analysis, and cluster analysis at the genus level showed that the community structure of the AY3550 sample belt and the other four sample belts differed greatly and could be divided into two successional stages. The enzyme activities of the 33 ACC-deaminase-producing bacteria isolated and purified from moss-covered soil at different altitudes ranged from 0.067 to 4.7375 U/mg, with strains DY1–3, DY1–4, and EY2–5 having the highest enzyme activities. All three strains were identified as Pseudomonas by morphology, physiology, biochemistry, and molecular biology. This study provides a basis for the changes in moss-covered soil microhabitats during glacial degradation under the synergistic effects of moss, soil, and microbial communities, as well as a theoretical basis for the excavation of valuable microorganisms under glacial moss-covered soils.

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Removal of environmental estrogens from wastewater by microalgae under the influence of bacteria

Sun,

Wang,

Xiong

et al. 2023

Journal of Cleaner Production

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Ectopic expression of bacterial 1-aminocyclopropane 1-carboxylate deaminase in Chlamydomonas reinhardtii enhances algal biomass and lipid content under nitrogen deficit condition

Cited by 5 publications

References 50 publications

The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions

The Zoige pioneer plant Leymus secalinus has different endophytic bacterial community structures to adapt to environmental conditions

Bacterial Diversity Analysis and Screening for ACC Deaminase-Producing Strains in Moss-Covered Soil at Different Altitudes in Tianshan Mountains—A Case Study of Glacier No. 1

Removal of environmental estrogens from wastewater by microalgae under the influence of bacteria

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