Ectopic osteogenic tissue formation by MC3T3-E1 cell-laden chitosan/hydroxyapatite composite scaffold

Abstract

This study evaluates the suitability of a macroporous three-dimensional chitosan/hydroxyapatite (CS/HA) composite as a bone tissue engineering scaffold using MC3T3-E1 cells. The CS/HA scaffold was produced by freeze-drying, and characterized by means of SEM and FTIR. In vitro findings demonstrated that CS/HA supported attachment and proliferation of cells, and stimulated extracellular matrix (ECM) production. Tissue biocompatibility and osteogenic capacity of the cell-laden constructs were evaluated in an ectopic Wistar rat model. In vivo results showed that the MC3T3-E1 cell-laden CS/HA was essentially histocompatible, promoted neovascularization and calcified matrix formation, and secreted osteoblast-specific protein. We conclude that the composite scaffold evaluated has potential for applications in bone regeneration.

Fabrication of dual drug loaded bilayered chitosan based composite scaffolds as osteochondral substitutes and evaluation of in vitro cell response using the MC3T3 pre-osteoblast cell line

“…This increase in fluorescence at day 8 confirmed that cells are metabolically active and started proliferation inside the pores of scaffolds. Similar results were previously reported by other researchers (Zhang et al 2012;Koç et al 2016). This also shows that the presence of both drugs has no effect towards cell viability as the drug release was continuous and its concentration increased with time.…”

Development and characterization of zinc‐incorporated montmorillonite/poly(ε‐caprolactone) composite scaffold for osteogenic tissue‐engineering applications

“…There are conflicting reports on ideal pore size . The optimal pore size is generally considered to be above 100 μm for inducing bone growth and about 300 μm for bone formation and vascularization . In this study, the pore size and size distribution of the composite scaffolds were evaluated by MIP and SEM analyses.…”

Effects of hydroxyapatite-coated nonwoven polyethylene/polypropylene fabric on non-mesodermal lineage-specific differentiation of human adipose-derived stem cells

“…Particulate HAp can have a highly porous structure, and its fibers can be electrospun and compounded with or without polymers to engineer scaffolds [20,32]. The influence of the Hap-based fabric on the differentiation of mesodermal lineage cell types such as osteocytes has been described [31][32][33]. Earlier studies demonstrated enhanced differentiation, supported attachment, and proliferation, and stimulated extracellular matrix (ECM) production [31,33].…”

“…HAp is the main mineral component of mammalian hard tissues (teeth and bones) and human renal calculi [ 30 ]. Particulate HAp can have a highly porous structure, and its fibers can be electrospun and compounded with or without polymers to engineer scaffolds [ 20 , 32 ]. The influence of the Hap-based fabric on the differentiation of mesodermal lineage cell types such as osteocytes has been described [ 31 – 33 ].…”