Before starting any physical activity, it is common to warm-up. However, the effects of including stretching in the warm-up remain controversial, and so does the most appropriate type of stretching depending on the activity to be performed afterwards. Thus, the aim of this article is to establish a systematic review protocol to analyze the effects of including different types of stretching in the warm-up on motor performance. The different basic physical abilities (strength, endurance, speed, flexibility) and coordinative abilities (coordination, balance, agility) will be analyzed. To this end, a systematic review will be carried out by examining eight electronic databases (Web of Science™ (all databases), Scopus, SPORTDiscus with Full Text, Pub-Med, CINAHL, Cochrane Library Plus, ProQuest Dissertations & Theses Global™ and WorldCat), along with carrying out a complementary search phase (snowballing). This will be followed by a meta-analysis, where each selected study will be coded (study characteristics, participant characteristics, intervention characteristics, intervention outcomes) using an ad hoc code established by the research group and previously tested on a sample of studies at the beginning of the review. To ensure the methodological quality of the systematic review, as well as its accuracy and transparency, the PRISMA-P and Cochrane Handbook protocol for systematic reviews will be employed.