Efectos de reserpina y p-cloranfetamina en el metabolismo y la liberación de 5-HT en el ganglio cerebral de Inachis io (Lepidoptera).

Abstract

There have been few pharmacological studies of serotonergic system dynamics in insects. A more precise knowledge of the response of serotonergic neurons to drugs will contribute to understanding of the role of this neurotransmitter in insect behaviour. The present work was carried out to study several aspects of serotonin (5-HT) metabolism and release in an insect, the butterfly Inachis io. The effects of a single intra-abdominal injection of reserpine (30 pg/insect) or p-chloroamphetamine (50 microg/insect) on cerebral ganglia 5-HT metabolism and release were studied. After reserpine injection a depletion of 5-HT stores concomitant with an increase in N-acetylserotonin levels was observed, but not significant alteration of extraneuronal 5-HT release was observed. Administration of p-chloroamphetamine (PCA) induced extraneuronal 5-HT release, together with inhibition of its reuptake. Finally, a single injection of p-chloroamphetamine in reserpine-treated insects was able to induce new release of 5-HT. Reserpine interferes with the vesicular storage of 5-HT, but does not affect the process of neuronal release, while PCA induces the synaptic release of 5-HT and inhibits its reuptake. These effects are similar to those observed in mammals.

Effects of reserpine on reproduction and serotonin immunoreactivity in the stable fly Stomoxys calcitrans (L.)

“…Reserpine selectively depleted octopamine, dopamine and serotonin in the nervous system of the cockroach, Periplaneta americana (Sloley and Owen, 1982; Sloley and Orikasa, 1988). The amine-depleting effect of reserpine has also been demonstrated in beetles, blow flies, honeybees, crickets, and butterflies (Masner et al, 1970; Brookhart et al, 1987; Menzel et al, 1999; Stevenson et al, 2000; Vieira et al, 2007). Reserpine was reported to act as a chemosterilant when fed to the Mediterranean fruit fly, Ceratitis capitata , where fertility and fecundity were interrupted (Cohen, 1983).…”

Production of reserpine of rauwolfia serpentina [L] kurz ex benth through in vitro culture enriched with plant growth regulators of NAA and kinetin

“…Result of a study showed that reserpine effect on the metabolism of serotonin in the brain ganglia of Inachisio [Lepidoptera] [7]. Demand of reserpine in Indonesia is increasing, but the supply of materials of this compound depending on availability of the plant growing in nature because this plant is rarely cultivated that the plant is now rarely found in nature [8]. Therefore, it is a need to cultivate the plant in order to produce up to commercial scale to meet the market demand.…”

Rapid screening and determination of designer drugs in saliva by a nib‐assisted paper spray‐mass spectrometry and separation technique

“…Substitutions to the amphetamine molecule give rise to a group of derivatives, and, as a result, a number of illegal, amphetamine‐like drugs are produced in underground labs for sale on the street . From the point of view of screening and the confirmation of amphetamine derivatives on the illicit market, more detailed detection and separation information would be highly desirable.…”