Production of reserpine of rauwolfia serpentina [L] kurz ex benth through in vitro culture enriched with plant growth regulators of NAA and kinetin

Abstract

Rauwolfia serpentine [L] Kurz ex Benth is a valuable plant yielding alkaloid of reserpine. The aim of this study was to find the most effective way of reserpine production. Growth regulators {naphthalene acetic acid [NAA] and Kinetin}were applied to promote and to regulate the growth of explant of R. serpentine cultured in vitro. The study was conducted at the laboratory of Bandung Institute of Technology [ITB]. Nine formulations of NAA and Kinetin were the treatments that were repeated twice in completely randomized design. Weight of callus was measured at one, two, three and four weeks after induction of explant. Reserpine analysis was performed by High Performance Liquid Chromatography [HPLC]. The result showed that the best callus induction was the treatment of 2.5 ppm NAA + 2.5 ppm Kinetin, and the highest content of reserpine was in root organ [0.0021 g / L], and in callus [0.0021 g / L] at the age of 4 weeks after induction. There was revealed that in vitro culture method was more productive in producing reserpine compound than the conventional plantation of R serpentine. Production of reserpine by callus culture was more effective and may be the basic for recommended further effort.

Indirect organogenesis and somatic embryogenesis for regeneration of Rauvolfia serpentina L. from root explants

“…2d). Subandil et al (2018) applied NAA and Kn to promote and to regulate the growth of explant of R. serpentina cultured. Bhadra et al (2013) reported that green and granular callus was produced on MS + 3.0 mg/l BAP + 1.0 mg/l NAA + 1.5 mg/l Kn from nodal explants of R. serpentina while loose and friable callus were produced on 0.1 mg/l picloram and 1.0 mg/l Kn containing medium from the same explants.…”

Growth and Yield of Cauliflower (Brassica Oleracea. L.) as an Effect of Water Supply and the Dosages of Rice Straw Mulch

“…Lack of water will cause nitrogen nutrient uptake to be inefficient and reduce the growth and yield of soybean crops (Agung and Rahayu, 2004). Soil environment lacking water in the first stages of growth may cause a retarded plantlet (Subandi and Dikayani, 2018).…”

Response Kailan Plants (Brassica oleraceae L.) to the Immersion Plant Growth Regulator (GA3) with Various Types Growing Media

“…Giberellins stimulate cell expansion through stimulation of cell wall enzymes, namely Xyloglucan Endotranslycosylase (XET) which can break the bonds in the cell wall-forming molecule, hemicelluluse which causes other wall components, namely cellulose microfibrils to move, causing widening or expansion of cell walls (Dengler, 2008;Subandi et al, 2018a), and this allows an increase in cell size which causes plants to grow taller.…”