Efek Larvasida Bakteri Kitinolitik dari Limbah Kulit Udang terhadap Larva Aedes aegypti

Widiastuti, Dyah; Marbawati, Dewi

doi:10.22435/aspirator.v8i1.4456.47-54

Cited by 5 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The water in larvae container become cloudy but not smell. This showed a better result compared to study by Widastuti, 9 which stated that the application of life chitinoly tic bacteria as biolarvacide causes the water in containers become muddy and stinky. Changes in water containers in the application of larvicides should be considered as the application of larvicides in community, especially for containers of water consumption should be sought not leading to a change in the water.…”

Section: Resultsmentioning

confidence: 63%

“…However, the LC 50 value of natural larvacide formula used in this study was lower if compared to Bucker et al, 24 study which reported that endophytic fungi extract and basidiomiset extract indicate LC 50 values at101.8 ppm and 156.8 ppm against Aedes aegypti larvae. In addition, the mixture of LC 50 Beauveria bassiana crude metabolites and enzyme chitinase is also lower than in study by Widastuti, 9 stating that LC 50 value of isolate BKUd01 against Aedes aegypti was achieved at concentration 2000 ppm. These differences indicate that application of Beauveria bassiana crude metabolites and enzyme chitinase can produce higher larvicidal effects than live isolated applications.…”

Section: Resultsmentioning

confidence: 68%

“…Thereby the community were preferred to drain their bath tubs instead of using larvacides. 9 Some weaknesses were found in the application of bi-…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae

Widiastuti¹,

Ikawati²,

Hadi³

2018

Kesmas: National Public Health Journal

Self Cite

View full text Add to dashboard Cite

Aedes aegypti is a major vector of dengue, a deadly disease causing deaths of millions of people in developing countries. Aedes aegypti control with chemical insecticide is widely used, and affects on a widespread insecticide resistance. Mosquito biological control was needed to replace the use of chemical insecticide. This study aimed to evaluate larvicidal effect of mixture of Beauveria bassiana crude metabolite and chitinase enzyme against Aedes aegypti larvae. An experimental study using completely randomized design was conducted in March-April 2016 at Banjarnegara Research and Development Unit for Zoonosis Control. Biolarvacide formula was made of mixture with 2 : 1 ratio between Beauveria bassiana crude metabolite and chitinase isolated from chitinolytic bacteria, which was propagated by the Agency's Bacteriology Laboratory. In experimental method, 120 Aedes aegypti larvae (the third instar) were exposed with four concentrations of biolarvacide formula (4%, 8%, 16%, and 32%) in 3 replicates. Results showed that exposure of biolarvacide formula caused the third instar larval mortality which started on the first day of exposure. Probit analysis showed LC 50 value was obtained at concentration of 53.2 ppm. This shows that larvacide formula of Beauveria bassiana and chitinase enzyme compounds are effective to be used as larvacides against Aedes aegypti larvae. Keywords: Aedes aegypti, Beauveria bassiana, larvacide AbstrakAedes aegypti merupakan vektor utama demam berdarah dengue (DBD) penyakit mematikan yang menyebabkan kematian jutaan orang di negara berkembang. Pengendalian Aedes aegypti dengan insektisida kimia banyak digunakan dan menimbulkan dampak resistensi insektisida yang meluas. Pengendalian nyamuk secara hayati diperlukan untuk menggantikan penggunaan insektisida kimia. Penelitian ini bertujuan untuk mengetahui efek larvasida campuran metabolit sekunder Beauveria bassiana dan enzim kitinase terhadap larva Aedes aegypti. Sebuah penelitian eksperimental dengan desain rancangan acak lengkap dilaksanakan pada bulan Maret-April 2016 di Balai Litbang P2B2 Banjarnegara. Formula larvasida hayati dibuat dari campuran dengan perbandingan 2:1 antara metabolit sekunder jamur Beauveria bassiana dan enzim kitinase yang diisolasi dari bakteri kitinolitik biakan Instalasi Bakteriologi Balai Litbang P2B2 Banjarnegara. Dalam metode eksperimen, sebanyak 120 ekor larva Aedes aegyppti (instar III) dipaparkan dengan empat konsentrasi formula larvasida hayati (4%, 8%, 16% dan 32%) dengan tiga buah ulangan. Hasil penelitian menunjukkan bahwa formula larvasida hayati menyebabkan kematian larva instar III Aedes aegypti yang dimulai pada hari pertama paparan. Hasil analisis probit menunjukkan nilai LC 50 campuran Beauveria bassiana dan enzim kitinase sebesar 53,2 ppm. Hal ini menunjukkan bahwa formula larvasida dari campuran Beauveria bassiana dan enzim kitinase efektif untuk digunakan sebagai larvasida terhadap jentik Aedes aegypti.

show abstract

Section: Resultsmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 68%

See 1 more Smart Citation

Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae

Widiastuti¹,

Ikawati²,

Hadi³

2018

Kesmas: National Public Health Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Pemanfaatan bakteri kitinolitik dari rendaman kulit udang dengan konsentrasi 4% dapat membunuh jentik Ae. aegypti sebesar 99% pada hari kedua (Widyastuti & Marbawati, 2016).…”

Section: -Ujiunclassified

Daya Tetas Telur Aedes aegypty Strain Japan Yang Disimpan Selama Seminggu Pada Suhu Ekstrem

2019

View full text Add to dashboard Cite

Dengue Hemorrhagic Fever (DHF) is one of the environmental health problems that several case increasing the number of patients and the wider area of distribution. The spread of dengue is influenced by several factors such as the vector disease, the behavior of people and the environment. In some sub-tropical countries is like in the winter season , a number of Aedes albopictus is found the eggs can still hatch at temperatures 0,5◦C. Eggs that will be tested for each treatment amounted to 100 eggs and had been through the process selected. The research carried in Parasitology and Entomology Labolatories, South Sumatra. The research was conducted from March to December 2014. In a multivariate test showed that the interaction of temperature and storage time affect the hatchability of eggs of Aedes aegypti strain Japan. Humidity and temperature can influence one of the insects are mosquitoes. At a certain temperature and humidity mosquitoes can not do the lifecycle and inhibite the morfology. The conclusion of this research is the cold storage and extreme temperature influence to eggs hacthingof Strain Japan the Aedes aegypti. Suggestions in this research is the public should continue to implement programs 3M plus, due to the Aedes eggs can survive in cold weather.

show abstract

“…aegypti. 2,3 Tahun 2014, penderita DBD pada 34 provinsi di Indonesia tercatat sebanyak 100.347 penderita dan 907 diantaranya meninggal dunia. Jumlah tersebut mengalami peningkatan menjadi yakni 126.675 penderita dan 1.229 orang diantaranya meninggal dunia pada Tahun 2015.…”

Section: Pendahuluanunclassified

Potensi Daya Tolak Ekstrak Daun Marigold (Tagetes erecta L.) terhadap Nyamuk Aedes aegypti

Marini¹,

Ni'mah

Mahdalena

et al. 2018

blb

View full text Add to dashboard Cite

Aedes aegypti is vector of Dengue Hemorrhagic fever (DHF) which still considered as important health problem in Indonesia. The marigold (T. erecta L.) is a well known plant in the community and widely used as traditional medicines and repellent mosquitoes plant. This study aims to assess the repellency effect of ethanol extract of marigold leaves againts Ae. aegypti. The marigold were obtained from the farmers’ garden in Kerinjing Village, Pagaralam, Sumatera Selatan Province. The extraction process was carried out in Pharmaceutical Laboratory and Genetics Laboratory Faculty of Mathematics and Natural Sciences, Sriwijaya University in June 2016. Maceration process used solvent ethanol 70%. The repellency test was done in Entomology Laboratory of Loka Litbang P2B2 Baturaja in August to October 2016. The repellent was made with mixture of cleansing milk as diluent with concentration of 25%, 30%, 35%, 40%, and 45%. The method of repellent test was referred to WHOPES 2009 with modification. The phytochemical test showed that marigold leaves extract had compound of alkaloid, flavonoid, saponin, and tannin. The repellency test showed that the lotion of marigold leaves extract was ineffective as repellent againts Ae. aegypti with complete protection up to more than 90% after two hours aplication at concentration 30%.

show abstract

Efek Larvasida Bakteri Kitinolitik dari Limbah Kulit Udang terhadap Larva Aedes aegypti

Cited by 5 publications

References 0 publications

Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae

Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae

Daya Tetas Telur Aedes aegypty Strain Japan Yang Disimpan Selama Seminggu Pada Suhu Ekstrem

Potensi Daya Tolak Ekstrak Daun Marigold (Tagetes erecta L.) terhadap Nyamuk Aedes aegypti

Contact Info

Product

Resources

About