Effect of a 12-Week Training Program on Levels of Glucose-6-Phosphate Dehydrogenase and Antioxidant Activity

Abstract

ÖzetAmaç: Bu çalışmanın amacı basketbol oyuncularında 12 haftalık aerobik egzersiz programının G6PD aktivitesi, glutation peroksidaz ve glutation S transveraz seviyeleri üzerine etkisi olup olmadığını araştırmak idi. Yöntem ve Gereçler: Başlangıç değerleri için basketbol oyuncuları ve sedanter yaş ve cins benzer sağlıklı kontroller için kan örnekleri alındı. Plazma glutation peroksidaz, glutation S transveraz ve G6PD aktiviteleri kitlerle değerlendirildi daha sonra basketbol oyuncularına 12 haftalık egzersiz programı uygulandı. Antrenman öncesi ve antrenman sonrası değerler tüm gruplarda karşılaştırıldı. Bulgular: Antrenman programından önce glutation peroksidaz, glutation S transveraz ve G6PD aktiviteleri basketbol oyuncuların-da sedanter kontrollerden anlamlı olarak yüksekti (p<0.05, p<0.05, p<0.001). Antrenman programından sonra G6PD aktiviteleri, glutation peroksidaz,glutation S transveraz seviyeleri anlamlı olarak artmıştı (p<0.05, p<0.01, p<0.01). G6PD aktivitesi ve glutation peroksidaz,glutation S transveraz seviyeleri arasında pozitif bir korelasyon bulundu. Sonuç: Sonuç olarak biz uzun süreli fiziksel egzersizlerin G6PD ve antioksidan aktiviteleri artırdığını ve antioksidan defansda adaptasyonlara neden olduğunu söyleyebiliriz. Abst ractObjective: It has been suggested that regular, long-term exercise increases erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and antioxidant enzymatic activities. The purpose of this study was to investigate whether G6PD activity and antioxidant activities consisting of glutathione peroxidase (GPX) and glutathione s transferase (GST) levels changed with a 12 week aerobic exercise program in basketball players. Materials and Methods:For baseline values, blood samples were obtained from basketball players and age and sex matched sedentary healthy volunteers. Plasma GPX, GST levels and G6PD activities were evaluated with commercial kits. Then, a 12 week training program was performed for basketball players. Pre training and post training values were compared within all groups. Results: Before training program, GPX, GST levels and G6PD activities were significantly higher in basketball players than sedentary controls (p<0.05, p<0.05, p<0.001, respectively). Also G6PD activities and GPX, GST levels were significantly increased after training program. (p<0.05, p<0.01, p<0.01, respectively). A positive correlation was found between G6PD activity and GPX, GST levels. Conclusion: Our results suggest that long term-regular physical exercise increases G6PD activity and antioxidant activities and cause adaptations in antioxidant defences. (Turk J Rheumatol 2010; 25: 34-6)

Effects of acute twelve minute run test on oxidative stress and antioxidant enzyme activities

“…In a study evaluating the effect of 12 week aerobic exercise program on G6PD activity, glutathione peroxidase and glutathione S transferase levels in basketball players, glutathione peroxidase levels were found to be increased after the exercise program (Kaldırımcı, 2010). In contrast, in the present study, there has been a slight decrease in GPx activity after the exercise which continued by decreasing after 24 h rest.…”