Effect of a dual‐head short‐stroke pump on post‐column peroxyoxalate chemiluminescence detection

Hayakawa, Kazuichi; Imaizumi, Noriko; Miyazaki, Motoichi

doi:10.1002/bmc.1130050403

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Chemiluminescence is a highly sensitive method and, therefore, the intensity significantly depends on the performance of the system. Hayakawa et al (1991) studied the effect of the HPLC pump and proposed that the dual-head short-stroke pump was advantageous for increasing the mixing efficiency of the solutions. We took advantage of their improvement.…”

Section: Discussionmentioning

confidence: 99%

A new sensitive determination method of estradiol in plasma using peroxyoxalate ester chemiluminescence combined with an HPLC system

Kuwahara

Takamatsu

Hayase³

2000

Biomed. Chromatogr.

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A new sensitive determination method of estradiol in a plasma sample using peroxyoxalate ester chemiluminescence was developed. Estradiol, which was extracted by liquid-liquid extraction using ethyl acetate from plasma, was derivatized with dansyl-chloride (DNS-Cl) and separated by reverse-phase HPLC. The performance of four oxalates, bis(trichlorophenyl)oxalate (TCPO), bis(2,4-dinitrophenyl)oxalate (DNPO), bis(pentafluorophenyl)oxalate (PFPO), and bis[4-nitro-2-(3,6, 9-trioxadecyloxycarbonyl)phenyl] oxalate (TDPO), were evaluated using the static system, and DNPO was found to have the most sensitive and stable chemiluminescence at a H(2)O(2) concentration of 30 mM. HPLC-chemiluminescence system using DNPO for the determination of estradiol was established. The detection limit of dansylated-estradiol (DNS-E2) was 15 fmol (4 pg) in the standard solution and 44 fmol (12 pg) in the rat plasma sample at S/N = 3.

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Section: Discussionmentioning

confidence: 99%

A new sensitive determination method of estradiol in plasma using peroxyoxalate ester chemiluminescence combined with an HPLC system

Kuwahara

Takamatsu

Hayase³

2000

Biomed. Chromatogr.

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“…In this system, a rotating flow mixing device having two directional inlets (MDI) (dead volume = 25 pL) (Kyowa Seimitsu, Tokyo, Japan) ( Fig. 2A) was used (Kobayashi and Imai, 1980b) with a DMX-23007' dual-head short-stroke pump (4.9 kL/stroke/head) (Sanuki, Tokyo, Japan) (P2) (Hayakawa et al, 1991;Sugiura et al. 1992) which delivered both the CL reagent solution (Rl, TDPO and hydrogen peroxide) and the imidazole buffer solution (R2).…”

Section: Chemicalsmentioning

confidence: 99%

Development of a chemiluminescence detection system using bis[4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl] oxalate for the sensitive determination of the fluorescent compounds separated with an acidic mobile phase

Sugiura

Kanda

Imai

1993

Biomedical Chromatography

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A rotating flow mixing device having three directional inlets was developed for a high performance liquid chromatographic peroxyoxalate chemiluminescence detection system for the sensitive detection of fluorescent compounds separated with acidic mobile phases, such as those containing trifluoroacetic acid. The diameters of the three inlets were 0.3, 0.3 and 0.5 mm i.d., for an acidic column eluate, chemiluminogenic reagents (bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl) phenyl] oxalate and hydrogen peroxide) and imidazole solution (for neutralization and as a catalyst), respectively. The three mixed solutions flowed out through an outlet (0.5 mm i.d.) at the centre of the top of the vessel. The reaction conditions to give the appropriate signal-to-noise ratio (S/N) were investigated using dipyridamole (an antiplatelet aggregation drug) as a typical fluorescent compound. The system was applied to the detection of dipyridamole and dansylated amino acids with a mobile phase containing trifluoroacetic acid. The detection limits for dipyridamole and each dansylated amino acid were 10 amol and sub-fmol (S/N = 2), respectively.

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Chemiluminescence of Organic Peroxides*The authors dedicate this chapter to the late Prof. Dr. Giuseppe Cilento (Universidade de São Paulo), whose untimely demise in 1994 saddened everyone who knew him as an excellent scientist and outstanding human being. This chapter is also dedicated to Prof. Waldemar Adam (Universität Würzburg, now ‘retired’ in Puerto Rico), who was directly or indirectly responsible for the research interests of the authors.

Baader

Stevani

Bastos

2009

Patai's Chemistry of Functional Groups

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Historical Introduction Basic Principles of Chemiluminescence Classification of Chemiluminescent Reactions Methods in Chemiluminescence Research General Mechanisms in Organic Chemiluminescence High‐Efficiency Organic Chemiluminescent Reactions Involving Peroxide Intermediates Acknowledgments

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Effect of a dual‐head short‐stroke pump on post‐column peroxyoxalate chemiluminescence detection

Cited by 6 publications

References 24 publications

A new sensitive determination method of estradiol in plasma using peroxyoxalate ester chemiluminescence combined with an HPLC system

A new sensitive determination method of estradiol in plasma using peroxyoxalate ester chemiluminescence combined with an HPLC system

Development of a chemiluminescence detection system using bis[4‐nitro‐2‐(3,6,9‐trioxadecyloxycarbonyl)phenyl] oxalate for the sensitive determination of the fluorescent compounds separated with an acidic mobile phase

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