Effect of a Multi-Species Probiotic on the Colonisation of Salmonella in Broilers

Neveling, Deon P.; Emmenes, Liesel van; Ahire, Jayesh J.; Pieterse, E.; Smith, Carine; Dicks, Leon M. T.

doi:10.1007/s12602-019-09593-y

Cited by 32 publications

(30 citation statements)

References 76 publications

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“…Considering the abundance at the genus level, Lactobacillus was found as the most abundant genus, constituting 22.8% of the microbiota in the fecal samples in the L. salivarius 3D8 scFv treated chickens. A combination of L. salivarius and Pediococcus parvulus also improved the weight gain, intestinal morphology, and immune response [ 94 ]. Neveling and co-authors have shown that a combination of Lactobacillus crispatus , L. salivarius , L. gallinarum , L. johnsonii , Enterococcus faecalis , and Bacillus amyloliquefaciens inhibited the colonization of Salmonella in the GIT of broilers.…”

Section: Gut Microbiotamentioning

confidence: 99%

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

Jha

Das

Oak

et al. 2020

Animals

200

140

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Probiotics are live microorganisms which, when administered in adequate amounts, confer health benefits to the host. The use of probiotics in poultry has increased steadily over the years due to higher demand for antibiotic-free poultry. The objective of this systematic review is to present and evaluate the effects of probiotics on the nutrient utilization, growth and laying performance, gut histomorphology, immunity, and gut microbiota of poultry. An electronic search was conducted using relevant keywords to include papers pertinent to the topic. Seventeen commonly used probiotic species were critically assessed for their roles in the performance and gut health of poultry under existing commercial production conditions. The results showed that probiotic supplementation could have the following effects: (1) modification of the intestinal microbiota, (2) stimulation of the immune system, (3) reduction in inflammatory reactions, (4) prevention of pathogen colonization, (5) enhancement of growth performance, (6) alteration of the ileal digestibility and total tract apparent digestibility coefficient, and (7) decrease in ammonia and urea excretion. Thus, probiotics can serve as a potential alternative to antibiotic growth promoters in poultry production. However, factors such as the intestinal health condition of birds, the probiotic inclusion level; and the incubation conditions, feedstuff, and water quality offered to birds may affect the outcome. This systematic review provides a summary of the use of probiotics in poultry production, as well as the potential role of probiotics in the nutrient utilization, growth and laying performance, and gut health of poultry.

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Section: Gut Microbiotamentioning

confidence: 99%

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

Jha

Das

Oak

et al. 2020

Animals

200

140

View full text Add to dashboard Cite

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“…In addition, previous studies have shown the ability of probiotics to modulate SCFA production, with regard to host health [11,12]. Dietary supplementation with a combination of microbial species, herein called multispecies probiotics (MSPs), was beneficial due to improved growth performance, reduction of incidence of diarrhea, and protection against pathogens in animals [13][14][15][16]. Although the modulatory effects of MSPs on host gut microbiota has been also examined in previous studies, there is still limited information on its effect on the modulation of gut microbiota and production of SCFAs in pigs, which is economically important in animal industry.…”

Section: Introductionmentioning

confidence: 99%

Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota

Vasquez

Kim

et al. 2021

J Anim Sci Technol

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Short-chain fatty acids (SCFAs) are metabolic products produced during the microbial fermentation of non-digestible fibers and play an important role in metabolic homeostasis and overall gut health. In this study, we investigated the effects of supplementation with multispecies probiotics (MSPs) containing Bacillus amyloliquefaciens, Limosilactobacillus reuteri, and Levilactobacillus brevis on the gut microbiota, and fecal SCFAs and lactate levels of weaned pigs. A total of 38 pigs weaned at 4 weeks of age were fed either a basal diet or a diet supplemented with MSPs for 6 weeks. MSP administration significantly increased the fecal concentrations of lactate (2.3-fold; p < 0.01), acetate (1.8-fold; p < 0.05), and formate (1.4-fold; p < 0.05). Moreover, MSP supplementation altered the gut microbiota of the pigs by significantly increasing the population of potentially beneficial bacteria such as Olsenella, Catonella, Catenibacterium, Acidaminococcus, and Ruminococcaceae. MSP supplementation also decreased the abundance of pathogenic bacteria such as Escherichia and Chlamydia. The modulation of the gut microbiota was observed to be strongly correlated with the changes in fecal SCFAs and lactate levels. Furthermore, we found changes in the functional pathways present within the gut, which supports our findings that MSP modulates the gut microbiota and SCFAs levels in pigs. The results support the potential use of MSPs to improve the gut health of animals by modulating SCFAs production.

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“…Carter et al [45] reported similar results when they used a mixture of Enterococcus faecium and Lactobacillus salivarius to inhibit the growth and colonization of S. Enteritidis. Neveling et al [46] revealed that multispecies probiotics composed of Lactobacillus crispatus, L. salivarius, Lactobacillus gallinarum, Lactobacillus johnsonii, Enterococcus faecalis, and Bacillus amyloliquefaciens could inhibit the colonization of S. Enteritidis in the intestine when administered to broiler chickens. The current results showed that the B. subtilis 2.0 g/L suspension was able to produce significant in vitro antimicrobial action against C. albicans (2.5×10 6 CFU/mL) and T. mentagrophytes (2.5×10 6 CFU/mL) after 2 h. The current results correlate well with those of Zhang et al [47], who reported that B. subtilis ANSB060 could neutralize the aflatoxins produced by Aspergillus pseudotamarii, Aspergillus flavus, Aspergillus niger, Aspergillus parasiticus, Aspergillus ochraceoroseus, and Aspergillus nomius and, when administered to broilers, could improve growth and increase weight gains.…”

Section: Discussionmentioning

confidence: 99%

Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers

2021

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Background and Aim: Probiotics improve intestinal balance through bacterial antagonism and competitive exclusion. This study aimed to investigate the in vitro antimicrobial activity, as well as the in vivo preventive, immunological, productive, and histopathological modifications produced by probiotic Bacillus subtilis. Materials and Methods: The in vitro antimicrobial activities of B. subtilis (5×106 CFU/g; 0.5, 1.0*, 1.5, and 2.0 g/L) were tested against Escherichia coli O157: H7, Salmonella Typhimurium, Candida albicans, and Trichophyton mentagrophytes after exposure times of 0.25, 0.5, 1, and 2 h using minimal inhibitory concentration procedures. A total of 320 1-day-old female Ross broiler chickens were divided into five groups. Four out of the five groups were supplemented with 0.5, 1.0*, 1.5, and 2.0 g/L probiotic B. subtilis from the age of 1 day old. Supplemented 14-day-old broiler chickens were challenged with only E. coli O157: H7 (4.5×1012 CFU/mL) and S. Typhimurium (1.2×107 CFU/mL). A total of 2461 samples (256 microbial-probiotic mixtures, 315 sera, 315 duodenal swabs, and 1575 organs) were collected. Results: The in vitro results revealed highly significant (p<0.001) killing rates at all-time points in 2.0 g/L B. subtilis: 99.9%, 90.0%, 95.6%, and 98.8% against E. coli, S. Typhimurium, C. albicans, and T. mentagrophytes, respectively. Broilers supplemented with 1.5 and 2.0 g/L B. subtilis revealed highly significant increases (p<0.01) in body weights, weight gains, carcass weights, edible organs' weights, immune organs' weights, biochemical profile, and immunoglobulin concentrations, as well as highly significant declines (p<0.01) in total bacterial, Enterobacteriaceae, and Salmonella counts. Histopathological photomicrographs revealed pronounced improvements and near-normal pictures of the livers and hearts of broilers with lymphoid hyperplasia in the bursa of Fabricius, thymus, and spleen after supplementation with 2.0 g/L B. subtilis. Conclusion: The studies revealed that 1.5-2.0 g of probiotic B. subtilis at a concentration of 5×106 CFU/g/L water was able to improve performance, enhance immunity, and tissue architecture, and produce direct antimicrobial actions.

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Effect of a Multi-Species Probiotic on the Colonisation of Salmonella in Broilers

Cited by 32 publications

References 76 publications

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

Multispecies probiotics alter fecal short-chain fatty acids and lactate levels in weaned pigs by modulating gut microbiota

Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers

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