Effect of a seashell protein Haishengsu on cell growth and expression of apoptosis genes in leukemia K562 cells

Li, Guangyao; Liu, Jizhu; Yu, Xinhua; Chen, Shuangfeng; Zhang, Bin; Zhang, Weifeng; Xiao, Taiwu; Wang, Chun Bo; Wang, Lexin

doi:10.25011/cim.v31i4.4783

Cited by 12 publications

(18 citation statements)

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“…It has a molecular weight of approximately 15 kDa but an unknown chemical structure. HSS was found to have a potent suppressive effect on several types of tumor cells in vivo and in vitro [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Liu

Chen²,

Zhang

et al. 2009

J Nat Med

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The aim of the study was to investigate the in vivo effect of the seashell protein Haishengsu (HSS) on Ehrlich ascites tumor. Mice were inoculated with Ehrlich ascites tumor cells and randomly divided into three HSS groups and a control group. The survival times in the three HSS-treated groups was longer than in the control (P < 0.01) and the increased life span in the high-dose HSS group was greater than in the lower-dose groups (P < 0.05). In comparison with control group, the mice receiving pretreatment of HSS had longer survival times and greater life spans following inoculation of the ascites tumor (P < 0.05). HSS therefore prolongs survival times and increases the life spans of mice bearing Ehrlich ascites tumor. Pretreatment with HSS also diminishes the detrimental effect of Ehrlich ascites tumor on the prognosis of these animals.

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Section: Introductionmentioning

confidence: 99%

Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Liu

Chen²,

Zhang

et al. 2009

J Nat Med

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“…In vitro studies found that HSS causes apoptosis and suppress cell growth at the G0/G1 and S phases of the K562 cell cycle. 4 Our recent study also demonstrated that with adriamycin-resistant K562/ADM cells, HSS also increased the rate of apoptosis. 12 In addition, HSS reduced the expressions of pglycoprotein, a drug-resistance inducing protein in leukemia and other cells.…”

Section: Discussionmentioning

confidence: 93%

“…[1][2][3] Recently, we found that HSS suppressed the growth of leukemia K562 cell by inhibiting the G0/G1 and S phases of the cell cycle. 4 HSS also induced apoptosis in these leukemia cells by reducing the expression of apoptosis suppressor bcl-2, and increasing the expression of apoptosis promoting bax. 4 The in vivo anticancer effects of HSS have been investigated in two small clinical trials.…”

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confidence: 99%

“…4 HSS also induced apoptosis in these leukemia cells by reducing the expression of apoptosis suppressor bcl-2, and increasing the expression of apoptosis promoting bax. 4 The in vivo anticancer effects of HSS have been investigated in two small clinical trials. 5,6 Daily intravenous administration of HSS for 4 weeks resulted in remission of non-small-cell lung cancer or renal cancer in approximately half of the patients.…”

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confidence: 99%

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Effect of Haishengsu on transplanted K562 and drug-resistant K562/ADM tumors: An experimental study

Liu

Yang²,

Li³

et al. 2009

CIM

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Clin Invest Med 2009; 32 (2): E117-E123. AbstractPurpose: To evaluate the effect of Haishengsu (HSS) on transplanted K562 and drug-resistant K562/ADM tumors. Methods: Mice were inoculated subcutaneously with K562 and K562/ADM cells, respectively. Tumour-bearing animals were divided into HSS, adriamycin, combination therapy (adriamycin plus HSS) and placebo groups. The anti-tumour effect was assessed by tumour growth curve and tumour inhibitory rate (IR). Results: In animals inoculated with K562 cells, the inhibitory rates of high (1800mg/kg) and medium (900mg/kg ) dose HSS groups were 100% and 96.4%, respectively, which was higher than that in the adriamycin (88.9%) or the combination therapy groups (85.8%, P<0.05). The inhibitory rate in the low-dose HSS group (53.4%) was lower than in all other groups (P<0.01). In mice inoculated with K562/ADM cells, the inhibitory rates in the high, medium and low dose HSS groups were 100%, 95.9%, and 44.1%, respectively. In the adriamycin group, the inhibitory rate was 23.07%, which was lower than in the HSS group (P < 0.01). Pathological examination of tumour tissues from HSS-treated animals showed extensive necrosis and bleeding. Conclusions: Haishengsu inhibits the growth of transplanted K562 tumours in mice. It is also effective in suppressing the growth of drug-resistant K562/ADM tumors in this animal model. Tegillarca granosa L. (Chinese name "xue han") is a species of seashell. It is widely distributed and used as a traditional Chinese medicine for cancer treatment in mainland China. Haishengsu (HSS) is a protein extract from Tegillarca granosa with a molecular weight of approximately 15 KDal. HSS has a potent suppressive effect on several types of tumor cells in vivo and

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“…HSS was also tested against MDR K562/ADR cells, and it induced apoptosis at 20 mg/l [178]. HSS also inhibited K562 cells at G0/G1 and S phase and lowered Bcl-2 and enhanced Bax levels ( Figure 2) (Table 6) [179].…”

Section: Saponinsmentioning

confidence: 99%

Natural Products for Treatment of Chronic Myeloid Leukemia

Khajapeer¹,

Baskaran²

2016

Anti-Cancer Drugs - Nature, Synthesis and Cell

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Chronic myeloid leukemia (CML) is a hematological malignancy that arises due to reciprocal translocation of 3′ sequences from c-Abelson (abl) protooncogene on chromosome 9 with 5′ sequence of truncated break point cluster region (bcr) to chromosome 22. The fusion gene product BCR-ABL, a functional oncoprotein p210, is a constitutively activated tyrosine kinase that activates several cell proliferative signaling pathways. BCR-ABL-specific tyrosine kinase inhibitors (TKIs) such as imatinib, nilotinib and ponatinib potently inhibit CML progression. However, drug resistance owing to BCR-ABL mutations and overexpression is still an issue. Natural products are chemical compounds or substances produced by living organisms. They are becoming an important research area for cancer drug discovery due to their low toxicity and cost-effectiveness. Several lines of evidence show that many NPs such as alkaloids, flavonoids, terpenoids, polyketides, lignans and saponins inhibit CML cell proliferation and induce apoptosis. NPs not only differentiate CML cells into monocyte/erythroid lineage but also can reverse the multi-drug resistance (MDR) in CML cells. In this chapter, we review the anti-CML activity of various NPs.

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Effect of a seashell protein Haishengsu on cell growth and expression of apoptosis genes in leukemia K562 cells

Cited by 12 publications

References 4 publications

Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Effect of Haishengsu on transplanted K562 and drug-resistant K562/ADM tumors: An experimental study

Natural Products for Treatment of Chronic Myeloid Leukemia

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