Effect of age on cytokine production in humans

Bernstein, Erica D.; Murasko, Donna M.

doi:10.1007/s11357-998-0024-6

Cited by 37 publications

(23 citation statements)

References 52 publications

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“…coli and HSV-1 concentrations that, in preliminary experiments, gave IL-6 levels that were approximately half way up the two dose response curves for maximal sensitivity. This is in keeping with Bernstein & Murasko’s finding that supraoptimal concentrations of stimuli tended to mask age-related differences in cytokine levels [ 30 ]. To monitor inter-assay variation we also included within every assay PBMCs that were frozen from healthy volunteers and tested with and without E .…”

Section: Resultssupporting

confidence: 90%

“…Before embarking on studies to compare the levels of CI (at zero time values) and immune responsiveness to bacterial, viral and T cell challenges in young and old healthy volunteers, we set out to optimize our assays. Although both PBMCs and whole blood samples have been used in the literature [ 30 – 35 ], we chose to use whole blood samples since they have been shown to more closely mimic in vivo conditions [ 34 ], and had the advantages of fewer manipulations and presence of autologous plasma (which likely affects responses to ex vivo challenges), granulocytes and red blood cells [ 36 ]. To avoid the effects of diurnal rhythms [ 28 ] and diet [ 37 ] on cytokine secretion, all blood samples were taken between 8:30 am–10:00 am from overnight-fasted volunteers.…”

Section: Resultsmentioning

confidence: 99%

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Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges

et al. 2017

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To identify reliable biomarkers of age-related changes in chronic inflammation and responsiveness to bacterial and viral challenges, we evaluated endogenous and ex vivo stimulated levels of 18 inflammatory markers, using whole blood collected in EDTA and sodium heparin tubes from 41 healthy volunteers, i.e., 11 men + 10 women aged 20–35 and 10 men + 10 women aged 50–77. These studies revealed significant differences in the levels of inflammatory markers when blood was collected in EDTA versus sodium heparin and age related differences in these biomarkers were confirmed with blood collected in EDTA from 120 healthy volunteers in 3 age categories, ie, 20 men + 20 women, aged 20–35, 36–49 and 50–77. Studies with unstimulated blood samples, to measure levels of chronic inflammation, revealed a significant increase with age in IL-12p70, CRP and PGE2, consistent with the concept of “inflammaging”, and a decrease in G-CSF in both men and women. Interestingly, in response to E. coli stimulation, PGE2 levels were markedly reduced in the 50–77 year old cohort while they were increased following Herpes Simplex virus-1 (HSV-1) stimulation, along with IL-8. In addition, unlike E. coli, HSV-1 potently stimulated IFNα production, but levels were dramatically reduced in the older cohort, consistent with a reduced ability to generate an anti-viral response. We also found platelets and CD8+ T cells were reduced with age while CD4+ T cells were significantly increased, resulting in a substantially higher CD4/CD8 ratio in the older cohort. Surprisingly, however, we found that the older cohort exhibited more T cell proliferation and IFNγ production in response to anti-CD3+anti-CD28 stimulation. Importantly, there was considerable person-to-person variation in these inflammatory markers in all age groups, making possible comparisons between a person’s “inflammage” and chronological age. These assays should help to identify individuals at high risk of autoimmune disorders and cancer.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges

et al. 2017

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“…Aging is accompanied by many changes in immune response, with the most consistent and dramatic alterations occurring within the T cell compartment. Since cytokines are central to immune cell communications, age-associated changes in cytokine production may contribute to these alterations [28]. According to the present study, the level of IL-12 decreased with age, while other study demonstrated that the age was not significantly correlated with the serum IL-12 [29].…”

Section: Discussioncontrasting

confidence: 46%

The Association of Severe Toxoplasmosis and Some Cytokine Levels in Breast Cancer Patients

Assim

Saheb

2018

ijs

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Toxoplasma gondii is an opportunistic pathogen in which the reactivation of a latent infection can cause death in congenitally infected fetuses, newborns, and immunocompromised patients. This study aimed to determine the seropositivity of toxoplasmosis infection and the possible association with Interleukin-12 (IL-12) and Interleukin-23 (IL-23) cytokines in breast cancer patients. In this study, 190 women were enrolled. All serum samples were tested for T. gondii immunoglobulins (IgG and IgM) antibodies and (IL-12, IL-23) levels using ELISA technique. The result of this study showed that breast cancer patients recorded the highest percentage of toxoplasmosis infection. There were no positivity rates for anti-Toxoplasma IgM in breast cancer patients while the positivity percentage for anti-Toxoplasma IgM among the control group was (7.00%). Furthermore, the seroprevalence of anti-Toxoplasma IgG was the highest in the age group (31-40) years in patients with breast cancer while the highest mean titer of the IL-12 is restricted to ages (21-30) years in the control groups who are seropositive to anti-Toxoplasma IgG. Although, in patients with breast cancer who are seropositive to anti-Toxoplasma IgG, the highest mean titer of the IL-23 was in ages (21-30) years. Since most immunosuppressive patients are exposed to various possible risk factors including Toxoplasma primary infection or reactivation, so it is important to diagnose and treat toxoplasmosis in breast cancer patients to reduce the consequences of the infection.

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“…So far, variation of cytokine production capacity in the general population has been investigated only in small studies, and this limitation resulted in conflicting conclusions (Aulock et al, 2006; Bernstein and Murasko, 1998; Grandgirard et al, 2013; Hwang et al, 2015; Nielsen et al, 2013; Scott et al, 2013). The only large scale studies of the immune system published to date are genome-wide studies focussed on the regulatory effect of genetic variation on cytokine gene transcription levels (eQTLs) rather than on protein expression levels (Berry et al, 2010; Fairfax et al, 2014; Lee et al, 2014; Raj et al, 2014; Ye et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Host and Environmental Factors Influencing Individual Human Cytokine Responses

Horst

Jaeger

Smeekens

et al. 2016

Cell

391

386

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Summary Differences in susceptibility to immune-mediated diseases are determined by variability in immune responses. In three studies within the Human Functional Genomics Project we assessed the effect of environmental and non-genetic host factors, of the genetic make-up of the host, and of the intestinal microbiome, on the cytokine responses in humans. We analyzed the association of these factors with circulating mediators and with six cytokines after stimulation with 19 bacterial, fungal, viral and non-microbial metabolic stimuli in 534 healthy subjects. In this first study we show a strong impact of non-genetic host factors (e.g. age and gender) on cytokine production and circulating mediators. Additionally, annual seasonality is found to be an important environmental factor influencing cytokine production. Alpha-1-antitrypsin concentrations partially mediate the seasonality of cytokine responses, whereas the effect of vitamin D levels is limited. The complete dataset has been made publicly available as a comprehensive resource for future studies.

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Effect of age on cytokine production in humans

Cited by 37 publications

References 52 publications

Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges

Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges

The Association of Severe Toxoplasmosis and Some Cytokine Levels in Breast Cancer Patients

Host and Environmental Factors Influencing Individual Human Cytokine Responses

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