Effect of Aging on Gastrin Receptor Gene Expression in Rat Stomach

Waki, Shinya; Kinoshita, Yoshikazu; Wang, He Yao; Asahara, Masahiro; Matsushima, Yumi; Hassan, Sazzad; Okada, Akihiko; Maekawa, Toru; Fukui, Hirokazu; Kawanami, Chiharu; Kishi, Kiyohiko; Chiba, Tsutomu

doi:10.1016/s0196-9781(97)00373-2

Cited by 14 publications

(14 citation statements)

References 19 publications

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“…Many investigators have shown the presence of gastrin receptors in the gastric mucosa of dogs, rats, guinea pigs and humans using receptor binding or molecular biological studies [3,13–27]. The strong expression of gastrin receptors, particularly in parietal and ECL cells, has been clearly shown by our previous study as well as those by other investigators [22–25,27]. However, no data are currently available that directly show the presence of gastrin receptors in proliferating cells in the neck zone, thus, a direct link between gastrin and gastric mucosal proliferation remains unelucidated.…”

Section: Introductionmentioning

confidence: 51%

“…Gastrin is reported to be a growth‐promoting factor for cells that express gastrin receptors [1]. We and others have shown that parietal, ECL and D cells are specific cell types that have gastrin receptors on their cell membranes [3,13,14,17,18,21–24,27]. We have also shown that ECL cells with gastrin receptors proliferate when stimulated with gastrin [9,23].…”

Section: Discussionmentioning

confidence: 68%

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Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection

et al. 2001

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Gastrin stimulates proliferation of progenitor cells in the neck zone of gastric fundic mucosa. However, whether it directly enhances this proliferation through its receptors remains unclear. We investigated the expression of gastrin receptors in neck zone proliferating cells in rat gastric fundic glands using a reverse transcription polymerase chain reaction (RT-PCR) coupled with laser capture microdissection and in situ RT-PCR. Gastrin receptor expression was identified in c-fos-expressing cells located in the neck zone, and results of the RT-PCR analysis argued against contamination by other cells, such as enterochromaffin-like, parietal or D cells. Supporting this finding, gastrin receptor gene expression was identified in the neck zone as well as base glands by in situ RT-PCR. Therefore, it is suggested that proliferating cells in the neck zone are stimulated directly by gastrin via their gastrin receptors. ß

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Section: Introductionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 68%

Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection

et al. 2001

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“…LPO as oxidative products have been shown to participate in tissue injury and chronic inflammation 26. Naturally, the relation between LPO level and aging has been suggested 27,28.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Cochinchina momordica Seed Extract on Gastric Acid Secretion and Morphologic Change in Aged Rat Stomach

Jo¹,

Kim²,

Nam³

et al. 2013

Gut Liver

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Background/AimsCochinchina momordica seed extract (SK-MS10) has a gastric protective effect. We aimed to assess the effect of SK-MS10 on gastric acid secretion with morphologic changes in the aged rat.MethodsAcid secretions were evaluated in the male F344 rats of four different ages (6-, 31-, 74-week, and 2-year). The 31-week-old rats were divided to three groups and continuously administered chow containing vehicle, SK-MS10 and lansoprazole, respectively. At the age of 74 weeks and 2 years, basal and stimulated acid was measured and the expression of mRNA and protein of H+-K+-ATPase were determined. The area of connective tissue of lamina propria was measured.ResultsBasal and stimulated gastric acid significantly decreased and connective tissue of lamina propria increased with age. The expression of mRNA and protein of H+-K+-ATPase significantly decreased with age. However, 74-week-old rats in the SK-MS10 group had higher stimulated gastric acid secretion than those in the vehicle and lansoprazole groups. In 2-year-old rats of SK-MS10 group, there was no increase of connective tissue.ConclusionsAs SK-MS10 kept the capacity of acid secretion as well as connective tissue area to comparable to young rats, it might valuable to perform further research regarding mechanism of SK-MS10 as an antiaging agent in the stomach.

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“…It has previously been shown that ECL cells have abundant gastrin receptors and that administration of gastrin stimulates the proliferation of ECL cells and the secretion of histamine from these cells. [17][18][19] Therefore, the short-term effect of gastrin on Reg production by rat ECL cells was investigated in vitro and in vivo. Both of these studies, using cultured ECL cells or subcutaneous administration of gastrin to rats, showed that gastrin directly stimulated Reg production by ECL cells.…”

Section: Regulation Of Gastric Reg Protein Productionmentioning

confidence: 99%

Reg protein is a unique growth factor of gastric mucosal cells

et al. 2004

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In 1984, Reg protein was shown to be stimulated during the regeneration of pancreatic islets. Since then, many Reg-related proteins have been identified in humans and other animals. These Reg-related proteins are classified into four subfamilies according to their amino-acid sequences, but they share a similar structure and physiological function. The role of Reg in gastric tissue was investigated, and Reg I was found to be expressed mainly in gastric fundic enterochromaffin-like (ECL) cells. Reg I production in ECL cells is stimulated by gastrin, as well as by the proinflammatory cytokine, cytokine-induced neutrophil chemoattractant (CINC)-2Beta. In patients with chronic hypergastrinemia, Reg production is stimulated, with the increased proliferation of gastric mucosal cells. Patients with Helicobacter pylori infection also showed increased Reg production in the gastric mucosa, partly via increased plasma gastrin concentration and partly via increased proinflammatory cytokine production. Thus, Reg protein induced by H. pylori infection may be partly responsible for the increased proliferation of gastric epithelial cells in H. pylori-infected patients. Reg protein is also produced in many gastric cancer cells, especially in poorly differentiated and advanced cancers. Reg protein stimulates the proliferation of several gastric cancer cell types, and gastric cancers with Reg protein expression tend to show a poorer clinical outcome. In summary, Reg protein may be a growth factor that regulates the proliferation and differentiation of normal and neoplastic gastric epithelial cells.

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Effect of Aging on Gastrin Receptor Gene Expression in Rat Stomach

Cited by 14 publications

References 19 publications

Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection

Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection

The Effect of Cochinchina momordica Seed Extract on Gastric Acid Secretion and Morphologic Change in Aged Rat Stomach

Reg protein is a unique growth factor of gastric mucosal cells

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